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Principles of Cryopreservation by Vitrification
2014Vitrification is an alternative approach to cryopreservation that enables hydrated living cells to be cooled to cryogenic temperatures in the absence of ice. Vitrification simplifies and frequently improves cryopreservation because it eliminates mechanical injury from ice, eliminates the need to find optimal cooling and warming rates, eliminates the ...
Gregory M, Fahy, Brian, Wowk
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Vitrification as an approach to cryopreservation
Cryobiology, 1984Recent developments have opened the possibilty that the problems of freezing and thawing organs might eventually be overcome by an alternative approach to organ cryopreservation, namely, vitrification. Here we will review some of the principles of vitrification, describe the current state of the art, consider how a practical vitrification scheme might ...
G M, Fahy +3 more
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Vitrification of Bovine Ovarian Tissue by the Solid-Surface Vitrification Method
Biopreservation and Biobanking, 2010Exposure time and addition of sucrose to the vitrification medium as well as the solid-surface vitrification (SSV) on the morphology of bovine preantral follicles were evaluated. Ovarian tissue was exposed for 1, 5, or 10 min to 4.0 M ethylene glycol with or without the addition of 0.5 M sucrose.
Juliana J H, Celestino +4 more
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Improved vitrification solutions based on the predictability of vitrification solution toxicity
Cryobiology, 2004Long-term preservation of complex engineered tissues and organs at cryogenic temperatures in the absence of ice has been prevented to date by the difficulty of discovering combinations of cryoprotectants that are both sufficiently non-toxic and sufficiently stable to allow viability to be maintained and ice formation to be avoided during slow cooling ...
Gregory M, Fahy +3 more
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Thermodynamic aspects of vitrification
Cryobiology, 2010Vitrification is a process in which a liquid begins to behave as a solid during cooling without any substantial change in molecular arrangement or thermodynamic state variables. The physical phenomenon of vitrification is relevant to both cryopreservation by freezing, in which cells survive in glass between ice crystals, and cryopreservation by ...
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Vitrification of a monatomic metallic liquid
Nature, 2007AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
M H, Bhat +6 more
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Parasite cryopreservation by vitrification
Cryobiology, 2004Parasitic protozoa and helminths and parasitic/vector insects each have distinct requirements for cryopreservation. Most parasitic protozoa respond to cryopreservation stresses similarly to other single cell suspensions, but few species are currently routinely cryopreserved by protocols specifically designed for vitrification.
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Vitrification of Equine Embryos
Veterinary Clinics of North America: Equine Practice, 2006Vitrification can be used successfully to cryopreserve equine embryos. Embryos for vitrification should be collected from donor mares' uteri when they are 300 mm or less in diameter, however,and at the morula or early blastocyst stage of development. No special equipment is required for vitrification; the straw containing the embryo is exposed to vapor
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Human Blastocyst Biopsy and Vitrification
Journal of Visualized Experiments, 2019Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the diagnostic techniques and to transfer the selected embryo(s) on a physiological endometrium in a following natural ...
Maggiulli R. +4 more
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