Segment 2 based characterization of a novel Indian Bluetongue virus isolate
Aim: The study was conducted to characterize and serotype the novel isolate of bluetongue virus (BTV) isolated from India. Materials and Methods: The BTV isolate was propagated in BHK-21 cell line.
Gaya Prasad +3 more
doaj +1 more source
Genetic Analysis of VP2 Gene of Canine Parvovirus Isolates in Korea
The purpose of this study was to genetically characterize CPV isolates from Korea. The VP2 gene of 31 isolates was characterized by DNA sequencing and their phylogeny. Among the 31 field CPV isolates, 28 isolates were classified as type 2a and other 3 isolates as type 2b. The isolates in 2a-I, II and III subclusters have unique mutations.
JEOUNG, Seok-Young +2 more
openaire +3 more sources
Construction of cell line stably expressing IBDV VP 2 protein mutants
A couple of primers was designed on the base of VP2 gene sequence of infectious bursal disease virus (IBDV) HZ2 strain, and the VP2 gene was successfully cloned into eukaryotic expression vector pDsRed2-N1.
SUN Jing +3 more
doaj +1 more source
Isolation of Canine parvovirus with a view to identify the prevalent serotype on the basis of partial sequence analysis [PDF]
Aim: The aim of this study was to isolate Canine parvovirus (CPV) from suspected dogs on madin darby canine kidney (MDCK) cell line and its confirmation by polymerase chain reaction (PCR) and nested PCR (NPCR).
Gurpreet Kaur +3 more
doaj +1 more source
Effect of a silencing suppressor gene towards the expression of VP2 protein of highly virulent infectious bursal disease virus in tobacco [PDF]
Gene silencing has been recognized as an important factor, which influence the expression of heterologous protein delivered via plant-viral based system.
Siti-Hasmah Mohtar +3 more
doaj
Molecular diagnosis and adaptation of highly virulent infectious bursal disease virus on chicken embryo fibroblast cell [PDF]
Aim: To collect Infectious bursal disease virus (IBDV) infected bursae based on postmortem findings of clinical samples followed by molecular confirmation and adaptation on chicken embryo fibroblast cell.
Yogender Singh +3 more
doaj +1 more source
Infectious bursal disease virus: identification of the novel genetic group and reassortant viruses
The results of the phylogenic analysis of the nucleotide sequence of the IBDV A and B genome segments have been presented. Traditionally the IBDV isolates are classified based on the phylogenic analysis of the hypervariable region of the VP2 gene.
L. O. Scherbakova +7 more
doaj +1 more source
Background Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies ...
Dao Chang +7 more
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Update on canine parvovirus: molecular and genomic aspects, with emphasis on genetic variants affecting the canine host [PDF]
Canine parvovirus (CPV), the etiology of hemorrhagic enteritis in dogs, was first isolated as CPV type 2 (CPV-2) almost 40 years ago, and was soon replaced by the emergence of new variant types. The major viral capsid proteins encoded by the VP2 gene are
Soulasack VANNAMAHAXAY +1 more
doaj +1 more source
Canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) cause severe disease in young animals, pups, and kittens. CPV-2 evolved from FPV by altering the species-specific binding of the viral capsid to the host receptor, i.e., the ...
Shaohan Li +7 more
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