Results 151 to 160 of about 1,145 (184)

Protective immune responses in rabbits induced by a suicidal DNA vaccine of the VP60 gene of rabbit hemorrhagic disease virus

Antiviral Research, 2013
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Chun Meng, Run Wu, Cheng Yingjie, Chuanfeng Li, Zongyan Chen, Guangqing Liu   +5 more
openaire   +3 more sources

[Tandem expression of the major epitope domains of the Moschus chrysogaster hemorrhagic disease virus VP60 and its protective efficacy to rabbits].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2020
Moschus chrysogaster (sifanicus) viral hemorrhagic disease (McVHD) is an acute and highly lethal infectious disease caused by Moschus chrysogaster hemorrhagic disease virus (McHDV) whose genome sequence is highly homologous with rabbit hemorrhagic disease virus. To screen the protective antigen of McHDV and set the basis for study of McVHD vaccine, the
Xiaoping Fu, Xiaochun Wu, Yangyang Zhang, Xiaoyong Xing, Fengqin Wen, Jian He, Jinyan Zhang, Shijun Bao   +7 more
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Obtaining recombinant capsid protein VP60 of rabbit haemorrhagic disease virus and its antigenic and immunogenic activity study

Veterinaria Kubani, 2020
Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus ...
Oleg Yu. Chernykh, Anna S. Moskvina, Oleg A. Verkhovskiy, Aleksey N. Mukhin, K P Alekseev, Ekaterina V Selezneva, Vetbiohim Llc   +6 more
openaire   +1 more source

Construction and immunogenicity of novel bivalent virus-like particles bearing VP60 genes of classic RHDV(GI.1) and RHDV2(GI.2)

Veterinary Microbiology, 2020
Rabbit hemorrhagic disease (RHD) is an acute, inflammatory, septic, and devastating infectious disease caused by Rabbit hemorrhagic disease virus (RHDV), which poses a serious threat to the rabbit industry. RHDV2 (GI.2/RHDVb), a recently reported new variant could cause RHD in wild populations, but also RHDV-vaccinated rabbits.
Qi, Ruibin, Miao, Qiuhong, Zhu, Jie, Tang, Jingyu, Tang, Aoxing, Wang, Xiaoxue, Dong, Dandan, Guo, Hongyuan, Liu, Guangqing   +8 more
openaire   +3 more sources

The amino terminal sequence of VP60 from rabbit hemorrhagic disease virus supports its putative subgenomic origin

Virus Research, 1993
Direct determination of the amino acid sequence of VP60 from rabbit hemorrhagic disease virus is impeded by the presence of a blocked N-terminus. Chemical cleavage of VP60 using cyanogen bromide allowed the identification and purification of two oligopeptides showing identical amino acid composition, one of which had its amino terminus blocked ...
Rosa Casais, José Antonio Boga, Francisco Parra, M. S. Marin   +3 more
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Immunogenic properties of rabbit haemorrhagic disease virus structural protein VP60 expressed by a recombinant baculovirus: an efficient vaccine

Virus Research, 1995
We have constructed a recombinant baculovirus containing the gene encoding the structural protein VP60 from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). Infection of cultured Spodoptera frugiperda Sf9 cells with this recombinant virus resulted in the production of high yields of VP60 protein which did not seem to ...
JoséL. Argüello-Villares, Ernest A. Gould, M. Soledad Marín, Luís I. Pérez Ordoyo García, JoséM. Martín Alonso, Rosa Casais, Wenrong Jiang, Francisco Parra, Karunakarannair Venugopal, J. Antonio Boga   +9 more
openaire   +3 more sources

Isolation and identification of a non-haemagglutinating strain of rabbit hemorrhagic disease virus from China and sequence analysis for the VP60 Gene

Virus Genes, 2007
A variant strain of rabbit hemorrhagic disease virus, designated "whn-1", was isolated and identified in China. The virus lacked haemagglutinating activity at 25, 37 and 4 degrees C, respectively, and gave negative results in the HAT after two passages in experimentally infected rabbits, but gave positive results in Agar Diffusion Reaction (ADR) and ...
Jian-wen Li, Wan-rong Zhou, Juan Liao, Lang Tian, Xia-lan Zhang, Hong-ning Wang, Hong-ning Wang   +6 more
openaire   +3 more sources

High-level expression and immunogenic properties of the recombinant rabbit hemorrhagic disease virus VP60 capsid protein obtained in Pichia pastoris

Journal of Biotechnology, 2005
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
Omar Farnós, Oscar Boué, Francisco Parra, José M. Martı́n-Alonso, Odaysa Valdés, Marisdania Joglar, Leonor Navea, Paula Naranjo, Ricardo Lleonart   +8 more
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Analysis of two VP60 capsid protein genes of rabbit hemorrhagic disease virus from viruses obtained from the same farm

Archives of Virology, 2010
Two distinct clones of the VP60 capsid protein gene of rabbit hemorrhagic disease virus were amplified from mixed liver tissue of rabbits collected from the same farm in the Xinjiang Uygur Autonomous Region of China in 2002. The results of DNA sequence analysis showed that the length of the VP60 gene in the first clone was 1,740 bp, similar to other ...
Li Yu, Wenge Ma
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Development of a peptide-generated antibody to rabbit hemorrhagic disease virus 2 VP60 and its immunohistochemical application in natural cases

Journal of Veterinary Diagnostic Investigation
Rabbit hemorrhagic disease virus 2 (RHDV2) has spread across the United States infecting and causing death in domestic and wild rabbits. Immunohistochemistry (IHC) would be a useful tool for the detection of RHDV2 antigen in tissues as it is inexpensive and readily achievable in most diagnostic laboratories.
Alisia A. W. Weyna, Natalie K. Stilwell, Rachel Anders, Brittany McHale, Rita McManamon, Elizabeth W. Howerth   +5 more
openalex   +3 more sources