Results 141 to 150 of about 1,200 (174)
Some of the next articles are maybe not open access.
Expression of truncated CTB::VP60 in tobacco exhibited no immunogenicity in rabbits
Plant Science, 2011Despite several optimizations, the production of CTB::VP60 antigen fusion proteins in tobacco is still very low. This might be due to the size of the fusion partner VP60 (579 aa). Hence, two different N-terminal truncations of VP60 were fused to CTB, either with or without an ER retention signal.
Inge Broer
exaly +3 more sources
Veterinary Microbiology, 2013
Rabbit hemorrhagic disease (RHD) is a contagious disease in adult rabbits, with high mortality, that occurs throughout the world. The VP60 protein has been implicated as main protein antigen in virus diagnosis and vaccine design. In this report, we describe the construction of a novel DNA vaccine (pcDNA-VP60) expressing the RHDV capsid protein (VP60 ...
Qian Jiang
exaly +3 more sources
Rabbit hemorrhagic disease (RHD) is a contagious disease in adult rabbits, with high mortality, that occurs throughout the world. The VP60 protein has been implicated as main protein antigen in virus diagnosis and vaccine design. In this report, we describe the construction of a novel DNA vaccine (pcDNA-VP60) expressing the RHDV capsid protein (VP60 ...
Qian Jiang
exaly +3 more sources
Detection of positive selection in the major capsid protein VP60 of the rabbit haemorrhagic disease virus (RHDV) [PDF]
Virus Research, 2008 Mutations were analysed in the major capsid protein VP60 of the rabbit haemorrhagic disease virus (RHDV), a calicivirus responsible for high mortality rates in both wild and domestic European rabbits (Oryctolagus cuniculus). Likelihood of positive selection was estimated using the PAML software applied to 43 non-identical complete sequences of the ...
Joana Abrantes +2 more
exaly +4 more sources
Virus Research, 1993
Direct determination of the amino acid sequence of VP60 from rabbit hemorrhagic disease virus is impeded by the presence of a blocked N-terminus. Chemical cleavage of VP60 using cyanogen bromide allowed the identification and purification of two oligopeptides showing identical amino acid composition, one of which had its amino terminus blocked ...
Francisco Parra +2 more
exaly +3 more sources
Direct determination of the amino acid sequence of VP60 from rabbit hemorrhagic disease virus is impeded by the presence of a blocked N-terminus. Chemical cleavage of VP60 using cyanogen bromide allowed the identification and purification of two oligopeptides showing identical amino acid composition, one of which had its amino terminus blocked ...
Francisco Parra +2 more
exaly +3 more sources
Antigenicity of VP60 structural proteinof rabbit haemorrhagic disease virus
Archives of Virology, 1997Five overlapping segments of the VP60 capsid protein gene of rabbit haemorrhagic disease virus have been expressed in E. coli under the control of the T7 RNA polymerase. After purification, the antigenicity of these denatured protein segments has been studied by reactivity with sera from both naturally infected and vaccinated animals in Western blot ...
E, Viaplana, J, Plana, A, Villaverde
openaire +2 more sources
Virus Research, 1995
We have constructed a recombinant baculovirus containing the gene encoding the structural protein VP60 from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). Infection of cultured Spodoptera frugiperda Sf9 cells with this recombinant virus resulted in the production of high yields of VP60 protein which did not seem to ...
Rosa Casais, Francisco Parra
exaly +3 more sources
We have constructed a recombinant baculovirus containing the gene encoding the structural protein VP60 from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). Infection of cultured Spodoptera frugiperda Sf9 cells with this recombinant virus resulted in the production of high yields of VP60 protein which did not seem to ...
Rosa Casais, Francisco Parra
exaly +3 more sources
VP60 The Importance Of Patient Organizations Involvement In Health Technology Assessment
International Journal of Technology Assessment in Health Care, 2017INTRODUCTION:In Europe, the work done by the European Commission and the European Network for Health Technology Assessment (EUnetHTA) has consolidated the role of Health Technology Assessment (HTA), and promises to better harmonize its impact across European Union (EU) countries.
Kathi Apostolidis +3 more
openaire +1 more source
Archives of Virology, 1996
Rabbit hemorrhagic disease virus (RHDV) causes more than 90% mortality in adult rabbits. In this study, the cDNA of the VP60 coding sequence of RHDV was cloned under the control of the polyhedrin and p10 promoters of baculovirus to be expressed in insect cells. The expression of RHDV VP60 under the control of the p10 promoter was 5-10 times higher than
J, Plana-Duran +6 more
openaire +2 more sources
Rabbit hemorrhagic disease virus (RHDV) causes more than 90% mortality in adult rabbits. In this study, the cDNA of the VP60 coding sequence of RHDV was cloned under the control of the polyhedrin and p10 promoters of baculovirus to be expressed in insect cells. The expression of RHDV VP60 under the control of the p10 promoter was 5-10 times higher than
J, Plana-Duran +6 more
openaire +2 more sources
Veterinary Microbiology, 2020
Rabbit hemorrhagic disease (RHD) is an acute, inflammatory, septic, and devastating infectious disease caused by Rabbit hemorrhagic disease virus (RHDV), which poses a serious threat to the rabbit industry. RHDV2 (GI.2/RHDVb), a recently reported new variant could cause RHD in wild populations, but also RHDV-vaccinated rabbits.
Qiuhong Miao, Jie Zhu, Guangqing Liu
exaly +4 more sources
Rabbit hemorrhagic disease (RHD) is an acute, inflammatory, septic, and devastating infectious disease caused by Rabbit hemorrhagic disease virus (RHDV), which poses a serious threat to the rabbit industry. RHDV2 (GI.2/RHDVb), a recently reported new variant could cause RHD in wild populations, but also RHDV-vaccinated rabbits.
Qiuhong Miao, Jie Zhu, Guangqing Liu
exaly +4 more sources
Egg yolk IgY against RHDV capsid protein VP60 promotes rabbit defense against RHDV infection
Veterinary Immunology and Immunopathology, 2014VP60 capsid protein is the major structural and immunogenicity protein of RHDV (Rabbit hemorrhagic disease virus, RHDV), and has been implicated as a main protein antigen in RHDV diagnosis and vaccine design. In this report, egg yolk antibody (IgY) against N-terminal of VP60 was evaluated and developed as a new strategy for RHDV therapy.
Zai Xin, Li +5 more
exaly +3 more sources