Results 141 to 150 of about 828 (163)
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Antiviral Research, 2013
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Chuanfeng Li +2 more
exaly +3 more sources
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Chuanfeng Li +2 more
exaly +3 more sources
Journal of Biotechnology, 2005
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
Francisco Parra, Ricardo Lleonart
exaly +3 more sources
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
Francisco Parra, Ricardo Lleonart
exaly +3 more sources
Polish Journal of Veterinary Sciences, 2012
In order to determine the genetic variability of Polish RHD virus strains and to confirm the presence of genetic variant (RHDVa) subtype the partial nucleotide sequences of capsid protein gene, including two highly variable regions C and E, were examined.
A, Fitzner +3 more
openaire +2 more sources
In order to determine the genetic variability of Polish RHD virus strains and to confirm the presence of genetic variant (RHDVa) subtype the partial nucleotide sequences of capsid protein gene, including two highly variable regions C and E, were examined.
A, Fitzner +3 more
openaire +2 more sources
Expression Behaviour of the Fusion Antigen CTB::VP60, A Promising RHD-Vaccine, in Pea
Journal Fur Verbraucherschutz Und Lebensmittelsicherheit, 2007The production of veterinary vaccines in transgenic plants appears to be an upcoming strategy. Since 15 years transgenic plants express a number of viral and bacterial antigens -amongst others VP60 (1, 2), the only structural capsid protein of the rabbit haemorrhagic disease virus (RHDV).
H. Mikschofsky +4 more
exaly +2 more sources
Virus Genes, 2007
A variant strain of rabbit hemorrhagic disease virus, designated "whn-1", was isolated and identified in China. The virus lacked haemagglutinating activity at 25, 37 and 4 degrees C, respectively, and gave negative results in the HAT after two passages in experimentally infected rabbits, but gave positive results in Agar Diffusion Reaction (ADR) and ...
Lang Tian
exaly +3 more sources
A variant strain of rabbit hemorrhagic disease virus, designated "whn-1", was isolated and identified in China. The virus lacked haemagglutinating activity at 25, 37 and 4 degrees C, respectively, and gave negative results in the HAT after two passages in experimentally infected rabbits, but gave positive results in Agar Diffusion Reaction (ADR) and ...
Lang Tian
exaly +3 more sources
Zastosowanie rekombinowanego białka VP60 wirusa RHD do immunizacji królików
Medycyna Weterynaryjna, 2005Rabbit haemorrhagic disease virus (RHDV) structural protein VP60 of Polish hemaglutinating isolate SGM was expressed using the baculovirus system. The recombinant protein self-assembled to form viruslike particles (VLPs). The VLPs were morfologically similar to the native virions and were able to agglutinate the human group 0 erythrocytes.
Andrzej Fitzner +4 more
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Egg Yolk IgY Against RHDV Capsid Protein VP60 Promotes Rabbit Defense Against RHDV Infection
Veterinary Immunology and Immunopathology, 2014VP60 capsid protein is the major structural and immunogenicity protein of RHDV (Rabbit hemorrhagic disease virus, RHDV), and has been implicated as a main protein antigen in RHDV diagnosis and vaccine design. In this report, egg yolk antibody (IgY) against N-terminal of VP60 was evaluated and developed as a new strategy for RHDV therapy.
Zai Xin, Li +5 more
exaly +3 more sources
Veterinaria Kubani, 2020
Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus ...
Aleksey N. Mukhin +5 more
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Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus ...
Aleksey N. Mukhin +5 more
openaire +1 more source
Archives of Virology, 2010
Two distinct clones of the VP60 capsid protein gene of rabbit hemorrhagic disease virus were amplified from mixed liver tissue of rabbits collected from the same farm in the Xinjiang Uygur Autonomous Region of China in 2002. The results of DNA sequence analysis showed that the length of the VP60 gene in the first clone was 1,740 bp, similar to other ...
Wenge, Ma, Li, Yu
exaly +3 more sources
Two distinct clones of the VP60 capsid protein gene of rabbit hemorrhagic disease virus were amplified from mixed liver tissue of rabbits collected from the same farm in the Xinjiang Uygur Autonomous Region of China in 2002. The results of DNA sequence analysis showed that the length of the VP60 gene in the first clone was 1,740 bp, similar to other ...
Wenge, Ma, Li, Yu
exaly +3 more sources
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2020
Rabbit haemorrhagic disease virus (RHDV) and myxoma virus (MYXV), are two pathogens that have harmful effect on rabbit breeding and population decline of European rabbits in their native range, causing rabbit haemorrhagic disease (rabbit fever) and myxomatosis, respectively. The capsid protein VP60 of the RHDV represents the major antigenic protein. To
Yuan, Wang +3 more
openaire +1 more source
Rabbit haemorrhagic disease virus (RHDV) and myxoma virus (MYXV), are two pathogens that have harmful effect on rabbit breeding and population decline of European rabbits in their native range, causing rabbit haemorrhagic disease (rabbit fever) and myxomatosis, respectively. The capsid protein VP60 of the RHDV represents the major antigenic protein. To
Yuan, Wang +3 more
openaire +1 more source