Results 151 to 160 of about 1,200 (174)
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Journal of Biotechnology, 2005
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
Omar Farnós +2 more
exaly +3 more sources
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
Omar Farnós +2 more
exaly +3 more sources
Archives of Virology, 2008
Rabbit haemorrhagic disease (RHD) is a highly fatal disease caused by a virus of the family Caliciviridae. Whereas recombination is well documented in other members of this family, the extent of recombination has so far not been studied in RHDV. To reach a better evaluation of the possible role of recombination in the evolution of RHDV virulence, we ...
J, Abrantes +2 more
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Rabbit haemorrhagic disease (RHD) is a highly fatal disease caused by a virus of the family Caliciviridae. Whereas recombination is well documented in other members of this family, the extent of recombination has so far not been studied in RHDV. To reach a better evaluation of the possible role of recombination in the evolution of RHDV virulence, we ...
J, Abrantes +2 more
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Expression behaviour of the fusion antigen CTB::VP60, a promising RHD-vaccine, in pea
Journal für Verbraucherschutz und Lebensmittelsicherheit, 2007The production of veterinary vaccines in transgenic plants appears to be an upcoming strategy. Since 15 years transgenic plants express a number of viral and bacterial antigens -amongst others VP60 (1, 2), the only structural capsid protein of the rabbit haemorrhagic disease virus (RHDV).
H. Mikschofsky +4 more
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Veterinaria Kubani, 2020
Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus ...
Aleksey N. Mukhin +5 more
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Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus ...
Aleksey N. Mukhin +5 more
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Plant Science, 2002
Abstract We have investigated the use of five different promoter-5′UTR leader combinations to develop a potato plant-based expression system for the production of VP60 protein from rabbit haemorrhagic disease virus (RHDV). The relative efficiency of CaMV 35S, modified CaMV 35S, sunflower polyubiquitin and patatin promoters, as well as the addition of
Francisco Parra
exaly +2 more sources
Abstract We have investigated the use of five different promoter-5′UTR leader combinations to develop a potato plant-based expression system for the production of VP60 protein from rabbit haemorrhagic disease virus (RHDV). The relative efficiency of CaMV 35S, modified CaMV 35S, sunflower polyubiquitin and patatin promoters, as well as the addition of
Francisco Parra
exaly +2 more sources
Zastosowanie rekombinowanego białka VP60 wirusa RHD do immunizacji królików
Medycyna Weterynaryjna, 2005Rabbit haemorrhagic disease virus (RHDV) structural protein VP60 of Polish hemaglutinating isolate SGM was expressed using the baculovirus system. The recombinant protein self-assembled to form viruslike particles (VLPs). The VLPs were morfologically similar to the native virions and were able to agglutinate the human group 0 erythrocytes.
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Polish Journal of Veterinary Sciences, 2012
In order to determine the genetic variability of Polish RHD virus strains and to confirm the presence of genetic variant (RHDVa) subtype the partial nucleotide sequences of capsid protein gene, including two highly variable regions C and E, were examined.
A, Fitzner +3 more
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In order to determine the genetic variability of Polish RHD virus strains and to confirm the presence of genetic variant (RHDVa) subtype the partial nucleotide sequences of capsid protein gene, including two highly variable regions C and E, were examined.
A, Fitzner +3 more
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Veterinary Microbiology, 2018
Rabbit hemorrhagic disease virus (RHDV) is responsible for rabbit hemorrhagic disease (RHD), which is an acute, lethal and highly contagious disease in both wild and domestic rabbits. Although current vaccines are highly effective for controlling RHD, they are derived from infected rabbit livers and their use is thus associated with safety and animal ...
Qian, Jiang +8 more
exaly +3 more sources
Rabbit hemorrhagic disease virus (RHDV) is responsible for rabbit hemorrhagic disease (RHD), which is an acute, lethal and highly contagious disease in both wild and domestic rabbits. Although current vaccines are highly effective for controlling RHD, they are derived from infected rabbit livers and their use is thus associated with safety and animal ...
Qian, Jiang +8 more
exaly +3 more sources
Antiviral Research, 2013
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Yingjie, Cheng +5 more
exaly +3 more sources
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Yingjie, Cheng +5 more
exaly +3 more sources
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2020
Rabbit haemorrhagic disease virus (RHDV) and myxoma virus (MYXV), are two pathogens that have harmful effect on rabbit breeding and population decline of European rabbits in their native range, causing rabbit haemorrhagic disease (rabbit fever) and myxomatosis, respectively. The capsid protein VP60 of the RHDV represents the major antigenic protein. To
Yuan, Wang +3 more
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Rabbit haemorrhagic disease virus (RHDV) and myxoma virus (MYXV), are two pathogens that have harmful effect on rabbit breeding and population decline of European rabbits in their native range, causing rabbit haemorrhagic disease (rabbit fever) and myxomatosis, respectively. The capsid protein VP60 of the RHDV represents the major antigenic protein. To
Yuan, Wang +3 more
openaire +1 more source