Results 131 to 140 of about 792 (161)
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Virus Research, 1995
We have constructed a recombinant baculovirus containing the gene encoding the structural protein VP60 from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). Infection of cultured Spodoptera frugiperda Sf9 cells with this recombinant virus resulted in the production of high yields of VP60 protein which did not seem to ...
M S Marin +2 more
exaly +3 more sources
We have constructed a recombinant baculovirus containing the gene encoding the structural protein VP60 from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). Infection of cultured Spodoptera frugiperda Sf9 cells with this recombinant virus resulted in the production of high yields of VP60 protein which did not seem to ...
M S Marin +2 more
exaly +3 more sources
Veterinary Microbiology, 2013
Rabbit hemorrhagic disease (RHD) is a contagious disease in adult rabbits, with high mortality, that occurs throughout the world. The VP60 protein has been implicated as main protein antigen in virus diagnosis and vaccine design. In this report, we describe the construction of a novel DNA vaccine (pcDNA-VP60) expressing the RHDV capsid protein (VP60 ...
Liandong Qu, Jiasen Liu, Qian Jiang
exaly +3 more sources
Rabbit hemorrhagic disease (RHD) is a contagious disease in adult rabbits, with high mortality, that occurs throughout the world. The VP60 protein has been implicated as main protein antigen in virus diagnosis and vaccine design. In this report, we describe the construction of a novel DNA vaccine (pcDNA-VP60) expressing the RHDV capsid protein (VP60 ...
Liandong Qu, Jiasen Liu, Qian Jiang
exaly +3 more sources
Virus Research, 1993
Direct determination of the amino acid sequence of VP60 from rabbit hemorrhagic disease virus is impeded by the presence of a blocked N-terminus. Chemical cleavage of VP60 using cyanogen bromide allowed the identification and purification of two oligopeptides showing identical amino acid composition, one of which had its amino terminus blocked ...
FRANCISCO PARRA +2 more
exaly +3 more sources
Direct determination of the amino acid sequence of VP60 from rabbit hemorrhagic disease virus is impeded by the presence of a blocked N-terminus. Chemical cleavage of VP60 using cyanogen bromide allowed the identification and purification of two oligopeptides showing identical amino acid composition, one of which had its amino terminus blocked ...
FRANCISCO PARRA +2 more
exaly +3 more sources
Egg yolk IgY against RHDV capsid protein VP60 promotes rabbit defense against RHDV infection
Veterinary Immunology and Immunopathology, 2014VP60 capsid protein is the major structural and immunogenicity protein of RHDV (Rabbit hemorrhagic disease virus, RHDV), and has been implicated as a main protein antigen in RHDV diagnosis and vaccine design. In this report, egg yolk antibody (IgY) against N-terminal of VP60 was evaluated and developed as a new strategy for RHDV therapy.
Xiao Yang Zhou
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Plant Science, 2002
Abstract We have investigated the use of five different promoter-5′UTR leader combinations to develop a potato plant-based expression system for the production of VP60 protein from rabbit haemorrhagic disease virus (RHDV). The relative efficiency of CaMV 35S, modified CaMV 35S, sunflower polyubiquitin and patatin promoters, as well as the addition of
JOSÉ Antonio Boga +2 more
exaly +2 more sources
Abstract We have investigated the use of five different promoter-5′UTR leader combinations to develop a potato plant-based expression system for the production of VP60 protein from rabbit haemorrhagic disease virus (RHDV). The relative efficiency of CaMV 35S, modified CaMV 35S, sunflower polyubiquitin and patatin promoters, as well as the addition of
JOSÉ Antonio Boga +2 more
exaly +2 more sources
Antiviral Research, 2013
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Zongyan Chen, Chuanfeng Li, Run Wu
exaly +3 more sources
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against rabbit hemorrhagic disease virus (RHDV). The VP60 gene of RHDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/VP60, was transfected into BHK-21 cells, and the antigenicity of the
Zongyan Chen, Chuanfeng Li, Run Wu
exaly +3 more sources
Journal of Biotechnology, 2005
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
OSCAR BOUE +2 more
exaly +3 more sources
The VP60 capsid protein from rabbit hemorrhagic disease virus (RHDV) (Spanish isolate AST/89) was cloned and expressed in Pichia pastoris. The transformed yeast was grown at high cell density and an expression level of about 1.5 g VP60L(-1) culture was obtained.
OSCAR BOUE +2 more
exaly +3 more sources
Archives of Virology, 2010
Two distinct clones of the VP60 capsid protein gene of rabbit hemorrhagic disease virus were amplified from mixed liver tissue of rabbits collected from the same farm in the Xinjiang Uygur Autonomous Region of China in 2002. The results of DNA sequence analysis showed that the length of the VP60 gene in the first clone was 1,740 bp, similar to other ...
Wenge, Ma, Li, Yu
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Two distinct clones of the VP60 capsid protein gene of rabbit hemorrhagic disease virus were amplified from mixed liver tissue of rabbits collected from the same farm in the Xinjiang Uygur Autonomous Region of China in 2002. The results of DNA sequence analysis showed that the length of the VP60 gene in the first clone was 1,740 bp, similar to other ...
Wenge, Ma, Li, Yu
exaly +3 more sources
Virus Genes, 2007
A variant strain of rabbit hemorrhagic disease virus, designated "whn-1", was isolated and identified in China. The virus lacked haemagglutinating activity at 25, 37 and 4 degrees C, respectively, and gave negative results in the HAT after two passages in experimentally infected rabbits, but gave positive results in Agar Diffusion Reaction (ADR) and ...
Juan Liao +2 more
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A variant strain of rabbit hemorrhagic disease virus, designated "whn-1", was isolated and identified in China. The virus lacked haemagglutinating activity at 25, 37 and 4 degrees C, respectively, and gave negative results in the HAT after two passages in experimentally infected rabbits, but gave positive results in Agar Diffusion Reaction (ADR) and ...
Juan Liao +2 more
exaly +3 more sources
Veterinary Microbiology, 2019
Rabbit hemorrhagic disease (RHD) is a highly contagious infection that has caused significant damage to the rabbit industry since 1984. Inactivated vaccines, the currently used prevention measures, are effective in controlling RHD. However, these vaccines are derived from the livers of infected rabbits, which constitutes a major concern in terms of ...
Yi Geng, Kaiyu Wang, Zexiao Yang
exaly +3 more sources
Rabbit hemorrhagic disease (RHD) is a highly contagious infection that has caused significant damage to the rabbit industry since 1984. Inactivated vaccines, the currently used prevention measures, are effective in controlling RHD. However, these vaccines are derived from the livers of infected rabbits, which constitutes a major concern in terms of ...
Yi Geng, Kaiyu Wang, Zexiao Yang
exaly +3 more sources