Results 171 to 180 of about 951,237 (293)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Dietary and water restriction leads to increased susceptibility to antimicrobial resistant pathogens. [PDF]

Sci Adv
Lacey KA, Pickrum AM, Gonzalez S, Bartnicki E, Castellaw AH, Rodrick TC, Jones DR, Khanna KM, Torres VJ.   +8 more
europepmc   +1 more source

Urinary markers of hydration during 3-day water restriction and graded rehydration. [PDF]

Eur J Nutr, 2020
Johnson EC, Huffman AE, Yoder H, Dolci A, Perrier ET, Larson-Meyer DE, Armstrong LE.   +6 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Salicylic acid improves cowpea productivity under water restriction in the field by modulating metabolism. [PDF]

Front Plant Sci
Cavalcante IE, de Melo AS, Ferraz RLS, de Alencar RS, Dias GF, Viana PMO, Rocha MM, Ndhlala AR, Sá FVDS, de Lacerda CF, Viégas PRA.   +10 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Early Growth and Physiological Acclimation to Shade and Water Restriction of Seven Sclerophyllous Species of the Mediterranean Forests of Central Chile. [PDF]

Plants (Basel)
Yáñez MA, Espinoza SE, Magni CR, Martínez-Herrera E.   +3 more
europepmc   +1 more source

Hyperadrenocorticism of calorie restriction contributes to its anti‐inflammatory action in mice [PDF]

, 2019
Allen, Brian D, Diaz, Vivian, Liao, Chen-Yu, Majzoub, Joseph A, Muglia, Louis J, Nelson, James F, Shu, Jianhua   +6 more
core   +2 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source