Results 91 to 100 of about 1,258,714 (290)

Diagnóstico molecular para Leishmaniasis

Revista Peruana de Medicina Experimental y Salud Pública, 1997
El potencial diagnóstico de epitopes inmunodominantes seleccionados fue ensayado satisfactoriamente a fin de obtener una prueba serodiagnóstica alternativa para la Leishmaniasis Tegumentaria Americana. Dos proteínas recombinantes prometedoras de L.
Ysabel Montoya, Carlos Padilla, Oscar Nolasco, Carlos León, Michael Talledo, Juana Choque, Omar Cáceres, Teresa Barreto, Maxy De Los Santos, lván Campos, Susan Douglas, Carlos Yabar, Douglas Barker   +12 more
doaj  

Leishmaniose em cães domésticos: aspectos epidemiológicos

Cadernos de Saúde Pública, 2005
Em Barra de Guaratiba, área endêmica de leishmaniose visceral americana (LVA) no Rio de Janeiro, Brasil, as campanhas de controle não têm sido capazes de reduzir a infecção canina.
Alba Valéria Machado da Silva, Adelzon Assis de Paula, Maria Alice Airosa Cabrera, João Carlos Araújo Carreira   +3 more
doaj   +1 more source

Evidence for the involvement of lipid rafts localized at the ER-mitochondria associated membranes in autophagosome formation [PDF]

, 2016
Mitochondria-associated membranes (MAMs) are subdomains of the endoplasmic reticulum (ER) that interact with mitochondria. This membrane scrambling between ER and mitochondria appears to play a critical role in the earliest steps of autophagy.
Faggioni, Alberto, Gambardella, Lucrezia, Garofalo, Tina, Malorni, Walter, Manganelli, Valeria, Marconi, Matteo, Matarrese, Paola, Misasi, Roberta, Sorice, Maurizio, Tinari, Antonella   +9 more
core   +2 more sources

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Proteasome inhibitor, ixazomib prevents topoisomerase‐I degradation and reverses irinotecan resistance in colorectal cancer

Molecular Oncology, EarlyView.
Ixazomib inhibits proteasome‐mediated degradation of topoisomerase I induced by irinotecan, thereby restoring drug sensitivity and promoting tumor cell death in colorectal cancer. Irinotecan, a topoisomerase I (topoI) inhibitor, is widely used for colorectal cancer, but resistance remains a major clinical challenge.
Yuho Ebata, Koji Ando, Hirofumi Hasuda, Koshi Mimori, Elizabeth C. Unan, Siddhartha Pulukuri, Aahana Tiku, Allison Berger, Eiji Oki, Ajit Bharti, Tomoharu Yoshizumi   +10 more
wiley   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Antigen retrieval by citrate solution improves western blot signal

MethodsX, 2019
In the present work, we describe and evaluate an additional step to the standard western blot protocol to increase signal strength after revealing. Weak or absence of signal is a common issue in western blot protocol leading to unexpected results. In our
Daniel Patiño-García, Nadia Rocha-Pérez, Ricardo D. Moreno, Renan Orellana   +3 more
doaj   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Production of polyclonal antiserum against beet western yellows virus coat protein expressed in Escherichia coli

Journal of Crop Protection, 2015
Serological methods are commonly used methods for detection of viruses. Preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies.
Arezu Lotfi, Masoud Shams-Bakhsh, Bagher Yakhchali   +2 more
doaj  

Preparation of Parasite Protein Extracts and Western Blot Analysis

Bio-Protocol, 2014
In order to prepare protein extracts of Plasmodium falciparum blood stages for western blot analysis, infected red blood cells (iRBC) need to be separated from uninfected red blood cells (uRBC) which make up the bulk of the parasite culture. Depending on
Arlett Heiber, Tobias Spielmann
doaj   +1 more source