Results 201 to 210 of about 3,088,136 (275)

Modulation of miR‐23b Wnt/β‐catenin Axis Strengthens Endothelial Barrier Properties

Advanced Science, EarlyView.
Early blood‐brain barrier (BBB) disruption contributes to stroke and CNS disease pathology. miR‐23b was identified as a regulator of BBB integrity in brain endothelial cells. Inhibition of miR‐23b enhanced barrier‐associated properties, promoted repair‐related signaling, and reduced BBB leakage in experimental stroke models, supporting further ...
Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, Douglas Jury, Brian Lawson, Jacob Körbelin, Lewis M. Brown, Vilas Menon, Dritan Agalliu, Irene Munk Pedersen   +16 more
wiley   +1 more source

Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue. [PDF]

Curr Protoc
Mungai M, Crabtree A, Le H, Moore J, Nguyen D, Rodriguez B, Harris C, Stephens DC, Beasley HK, Garza-Lopez E, Neikirk K, Shao B, Oliver A, Wilson G, Bacevac S, Vang L, Vue Z, Vue N, Marshall AG, Turner K, Zaganjor E, Shao J, Murray S, Gaddy JA, Wanjalla C, Davis J, Damo SM, Banks LD, Hinton A.   +28 more
europepmc   +1 more source

Copper‐Driven Epithelial Barrier Disruption: A Novel Mechanism of COPD Acute Exacerbations Mediated by the TNF‐α/ATP7A Axis

Advanced Science, EarlyView.
This study reveals a copper‐driven mechanism of COPD acute exacerbations mediated by the TNF‐α/ATP7A axis. TNF‐α suppresses the copper transporter ATP7A via competitive NF‐κB/CREB1/CBP signaling, resulting in intracellular copper accumulation, mitochondrial oxidative stress, and epithelial barrier disruption.
Xinru Xiao, Yongzhe Hao, Ziqi Ding, Zhipeng Wang, Yujia Shi, Yanhua Huang, Chunjian Qi, Qian Zhang   +7 more
wiley   +1 more source

Ghostbuster-A Western Blot-Based Panel Method to Resolve False-Positive Brucellosis Serology Test Results. [PDF]

Microorganisms
Bányász B, Antal J, Dénes B.
europepmc   +1 more source

A Soft, Flexible Implant for Wireless Photothermal–Pyroelectric Neurostimulation

Advanced Science, EarlyView.
A replication strategy is used to enlarge the exposed surface area of barium titanate within flexible films with embedded carbon nanotubes, enabling efficient photothermal‐pyroelectric stimulation. Polar crystalline nanosheets show superior performance in guiding neural progenitor cells toward neuronal differentiation.
Jiang Wu, Minmin Mao, Beltzane Garcia Cirera, Hao Ye, Xiangzhong Chen, Josep Puigmartí‐Luis, Ni Qin, Salvador Pané   +7 more
wiley   +1 more source

Fluorescence-Based Multiplex Western Blot to Simultaneously Detect the Insulin-Like Growth Factor-1 (IGF-1) Isoforms. [PDF]

Electrophoresis
Bocconcelli M, Fanelli F, Saltarelli R, De Santi M, Barone R, Barbieri E, Annibalini G.   +6 more
europepmc   +1 more source

BAP31 Drives Cartilage Calcification through Disruption of Autophagosome–Lysosome Fusion in Osteoarthritis

Advanced Science, EarlyView.
Mechanical stress activates BAP31 in chondrocytes. BAP31 competes with ATG14 for binding to STX17, disrupting the STX17–ATG14 complex required for autophagosome–lysosome fusion. The resultant autophagic flux blockade drives the generation of autophagy‐derived exosomes, which mediate pathological cartilage calcification in OA. Chondrocyte‐targeted BAP31
Zhi‐hua Xu, Ruo‐xin Wang, Feng He, Qian Liu, Fu‐yin Li, Min Hui, Yu‐qian Shi, Yu Jiang, Qing‐hua Li, Jun‐wen Jing, Shi‐bin Yu, Li‐tian Ma, Qian‐nan Niu, Mian Zhang   +13 more
wiley   +1 more source

14-3-3ζ: an optimal housekeeping protein for western blot analysis in swine rotator cuff tendon studies. [PDF]

Mol Cell Biochem
Rajalekshmi R, Rai V, Agrawal DK.
europepmc   +1 more source

Synthetic Toll‐Like Receptors for Control of Innate Immunity With Far‐Red Light

Advanced Science, EarlyView.
Synthetic Toll‐like receptors (TLRs) are engineered by replacing their natural sensing regions with a far‐red‐light‐responsive module from a bacterial phytochrome. These synthetic receptors enable light‐controlled regulation of immune signaling in mammalian cells.
Anna V. Leopold, Vladislav V. Verkhusha
wiley   +1 more source

A guide to selecting high-performing antibodies for Heterogeneous nuclear ribonucleoproteins A2/B1(hnRNP A2/B1) (UniProt ID: P22626) for use in western blot, immunoprecipitation, and immunofluorescence. [PDF]

F1000Res
Moleón VR, Ayoubi R, Alende C, Fothouhi M, Ryan J, Bolívar SG, Worrall D, Durcan TM, Brown CM, Francis V, McPherson PS, Laflamme C.   +11 more
europepmc   +1 more source