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A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence. [PDF]
F1000ResGonzález Bolívar S +8 more
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Development and validation of a chemiluminescent western blot assay for glanders (Burkholderia mallei) serodetection. [PDF]
J Vet Diagn InvestGrause JF +3 more
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A guide to selecting high-performing antibodies for Rab10 (UniProt ID: P61026) for use in western blot, immunoprecipitation, and immunofluorescence. [PDF]
F1000ResRuíz Moleón V +7 more
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Methods, 2006
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly.
Biji T, Kurien, R Hal, Scofield
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Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly.
Biji T, Kurien, R Hal, Scofield
openaire +3 more sources
Analytical Biochemistry, 1987
A method for successfully removing primary and secondary antibodies from nitrocellulose blots while preserving the originally immobilized polypeptides was developed. Polypeptides were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and electrophoretically transferred to nitrocellulose. Nonspecific binding sites
S H, Kaufmann, C M, Ewing, J H, Shaper
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A method for successfully removing primary and secondary antibodies from nitrocellulose blots while preserving the originally immobilized polypeptides was developed. Polypeptides were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and electrophoretically transferred to nitrocellulose. Nonspecific binding sites
S H, Kaufmann, C M, Ewing, J H, Shaper
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2003
Western blotting is employed for the detection of proteins and other macromolecules immobilized on nitrocellulose membranes. This rapid and sensitive method enables the identification and quantification of a specific protein from cell lysates or a mixture of proteins.
N R, Madamanchi, M S, Runge
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Western blotting is employed for the detection of proteins and other macromolecules immobilized on nitrocellulose membranes. This rapid and sensitive method enables the identification and quantification of a specific protein from cell lysates or a mixture of proteins.
N R, Madamanchi, M S, Runge
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Molecular Biotechnology, 1994
The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
Egger D, Bienz K
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The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described.
Egger D, Bienz K
openaire +3 more sources