Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems [PDF]
BioTechniques, 2023 Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues.
Rasheed Sule, Aldrin V Gomes
exaly +4 more sources
Comprehensive Optimization of Western Blotting [PDF]
Gels, 2023 Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu +3 more
doaj +2 more sources
Comparison of Automated and Traditional Western Blotting Methods [PDF]
Methods and Protocols, 2023 Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been developed.
Aino Sormunen +5 more
doaj +2 more sources
Detection of Zebrafish Retinal Proteins by Infrared Western Blotting [PDF]
Bio-Protocol, 2023 The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research.
Jingjing Zang, Stephan Neuhauss
doaj +2 more sources
Measuring protein levels in planarians using western blotting [PDF]
STAR Protocols, 2021 Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ...
Benjamin Ziman, Néstor J. Oviedo
doaj +2 more sources
Single-cell western blotting [PDF]
Nature Methods, 2014 To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h.
Alex J Hughes +2 more
exaly +4 more sources
Western Blotting and Immunoprecipitation of Native Human PIEZO1 Channels [PDF]
Bio-ProtocolPIEZO1 is a mechanically activated ion channel essential for mechanotransduction and downstream signaling in almost all organ systems. Western blotting is commonly used to study the expression, stability, and post-translational modifications of proteins.
Jinyuan Vero Li +2 more
doaj +2 more sources
Prognostic Value of Immunoglobulin G (IgG) Patterns by Western Blotting Immunodetection in Treated Dogs Previously Infected with Leishmania infantum [PDF]
Veterinary Sciences, 2021 Leishmaniasis is a heterogeneous group of neglected tropical diseases with various clinical syndromes, which is caused by obligate intracellular protozoa of the genus Leishmania and transmitted by the bite of a female phlebotomine sandfly.
Ehab Kotb Elmahallawy +6 more
doaj +2 more sources
Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence [PDF]
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
doaj +2 more sources
DropBlot: single-cell western blotting of chemically fixed cancer cells [PDF]
Nature CommunicationsArchived patient-derived tissue specimens play a central role in understanding disease and developing therapies. To address specificity and sensitivity shortcomings of existing single-cell resolution proteoform analysis tools, we introduce a hybrid ...
Yang Liu, Amy E. Herr
doaj +2 more sources