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Comparison of Automated and Traditional Western Blotting Methods [PDF]
Methods and Protocols, 2023 Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been developed.
Aino Sormunen +5 more
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Comprehensive Optimization of Western Blotting [PDF]
Gels, 2023 Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results.
Dishiwen Liu +3 more
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Detection of ASC Oligomerization by Western Blotting [PDF]
Bio-Protocol, 2017 The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1.
Jérôme Lugrin, Fabio Martinon
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Western blotting: an introduction. [PDF]
Methods Mol Biol, 2015 Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979.
Kurien BT, Scofield RH.
europepmc +6 more sources
Sensitive detection of fluorescence in western blotting by merging images. [PDF]
PLoS ONE, 2018 The western blotting technique is widely used to analyze protein expression levels and protein molecular weight. The chemiluminescence method is mainly used for detection due to its high sensitivity and ease of manipulation, but it is unsuitable for ...
Yukari Kondo +8 more
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Comparison of the sensitivity of Western blotting between PVDF and NC membranes. [PDF]
Sci Rep, 2021 Western blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the ...
Xiang Y +5 more
europepmc +2 more sources
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
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Detection of Zebrafish Retinal Proteins by Infrared Western Blotting [PDF]
Bio-Protocol, 2023 The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research.
Jingjing Zang, Stephan Neuhauss
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Western blotting (immunoblotting): history, theory, uses, protocol and problems
BioTechniques, 2023 Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues.
Rasheed Sule +2 more
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Transmembrane protein western blotting: Impact of sample preparation on detection of SLC11A2 (DMT1) and SLC40A1 (ferroportin). [PDF]
PLoS One, 2020 Western blotting has been widely used for investigation of protein expression, posttranslational modifications, and interactions. Because western blotting usually involves heat-denaturation of samples prior to gel loading, clarification of detailed ...
Tsuji Y.
europepmc +2 more sources