Molecular Oncology, EarlyView.Stemness properties, including quiescence, self‐renewal, and chemoresistance, are closely associated with leukemia relapse. Here, we demonstrate that DNA damage‐inducible transcript 4 (DDIT4) is induced in the hypoxic bone marrow niche and is essential for maintaining the stemness of AML1‐ETO9a leukemia cells.
Yishuang Li +12 more
wiley +1 more source
Use of the ELISA (Em2-Em18) and Western Blotting Methods on Diagnosis of Alveolar Echinococcosis
Türkiye Parazitoloji Dergisi, 2019 Objective:Alveolar echinococcosis (AE) is one of the most lethal parasitic zoonoses in the Northern Hemisphere, and early serological detection is important to start treatment and to improve survival.
Mehtap Demirkazık +4 more
doaj +1 more source
Real-time bio-layer interferometry ubiquitination assays as alternatives to western blotting. [PDF]
Anal Biochem, 2023 De Silva ARI, Shrestha S, Page RC.
europepmc +1 more source
Analysis of purified human liver α-
, 1992 Steven W. Johnson +4 more
openalex +1 more source
Gut alterations in a chronic kidney disease rat model with diet‐induced vascular calcification
FEBS Open Bio, EarlyView.Chronic kidney disease (CKD) patients often suffer from intestinal and/or mineral and bone disorders. Using a rat model, we showed that uremic vascular calcification is associated with gut barrier alterations (decreased gut mucus production and Nlrp6 gene expression, increased gut inflammation), and plasma retention of gut‐origin uremic toxins (indoxyl
Piotr Bartochowski +13 more
wiley +1 more source
Quantification Method of P2X3 Receptors in Rat DRG Neurons: Western Blotting [PDF]
, 2017 Skeletal muscle contractions are known to evoke pressor and cardioaccelerator responses in part by stimulating P2X3 receptors found on the peripheral endings of afferents. In diabetic patients, this pressor response is exaggerated.
Harrison, Michelle L. +2 more
core +1 more source
FEBS Open Bio, EarlyView.Two biomarkers of Alzheimer's disease, amyloid β‐peptide (Aβ) and tau, induce the transformation of U‐251 and other glioblastoma cell lines into neurotoxic A1‐like reactive astrocytes. This transformation is produced by cytokines and is followed by upregulation of PMCA activity and isoform expression, and is closely associated with inflammation, as ...
María Berrocal +2 more
wiley +1 more source
Detection of immunoglobulin M antibodies to glycoprotein G-2 by western blot (immunoblot) for diagnosis of initial herpes simplex virus type 2 genital infections [PDF]
, 1993 David Ho +4 more
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FEBS Open Bio, EarlyView.In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna +4 more
wiley +1 more source
Development of 4T1 breast cancer mouse model system for preclinical carbonic anhydrase IX studies
FEBS Open Bio, EarlyView.Carbonic anhydrase IX (CAIX) is a well‐recognised therapeutic target and prognostic biomarker in cancer. We developed and characterised a robust murine breast cancer model system that is suitable for CAIX studies in vitro and in vivo—it comprises both CAIX‐positive and CAIX‐negative controls and provides a solid platform for the comprehensive ...
Zane Kalniņa +13 more
wiley +1 more source