Single-cell western blotting [PDF]
Nature Methods, 2014 To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h. A microscope slide supporting a 30-μm-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into microwells, lysis in situ, gel electrophoresis ...
Hughes, Alex J +5 more
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Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation. [PDF]
Physiol Rep, 2020 The detection of erythropoietin (Epo) protein by Western blotting has required pre-purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation.
Yasuoka Y +17 more
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Measuring protein levels in planarians using western blotting [PDF]
STAR Protocols, 2021 Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ...
Benjamin Ziman, Néstor J. Oviedo
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Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting. [PDF]
Res Pract Thromb Haemost, 2020 Western blotting is used to measure protein expression in cells and tissues. Appropriate interpretation of resulting data is contingent upon antibody validation.
Rosell A +7 more
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A fixation method for the optimisation of western blotting. [PDF]
Sci Rep, 2019 Western blotting is the most extensively used technique for the identification and characterisation of proteins and their expression levels. One of the major issues with this technique is the loss of proteins from the blotted membrane during the ...
Xu J +11 more
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Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). [PDF]
PLoS ONE, 2015 Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity
Jennifer E Gilda +5 more
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Emerging techniques of western blotting for purification and analysis of protein
Future Journal of Pharmaceutical Sciences, 2021 Background Western blotting is frequently employed in molecular techniques like Proteomics and Biology. Because it is a sequential framework, differences and inaccuracies could even take place at any stage, decreasing this particular method's ...
Krishna Kumar Singh +3 more
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Prognostic Value of Immunoglobulin G (IgG) Patterns by Western Blotting Immunodetection in Treated Dogs Previously Infected with Leishmania infantum [PDF]
Veterinary Sciences, 2021 Leishmaniasis is a heterogeneous group of neglected tropical diseases with various clinical syndromes, which is caused by obligate intracellular protozoa of the genus Leishmania and transmitted by the bite of a female phlebotomine sandfly.
Ehab Kotb Elmahallawy +6 more
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Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence [PDF]
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
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Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP). [PDF]
Electrophoresis, 2019 Immunoassays such as ELISAs and Western blotting have been the common choice for protein validation studies for the past several decades. Technical advancements and modifications are continuously being developed to enhance the detection sensitivity of ...
Mishra M +5 more
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