Potassium-based algorithm allows correction for the hematocrit bias in quantitative analysis of caffeine and its major metabolite in dried blood spots [PDF]
, 2014 Although dried blood spot (DBS) sampling is increasingly receiving interest as a potential alternative to traditional blood sampling, the impact of hematocrit (Hct) on DBS results is limiting its final breakthrough in routine bioanalysis.
Capiau, Sara +4 more
core +2 more sources
Zika Virus Infection and Prolonged Viremia in Whole-Blood Specimens
Emerging Infectious Diseases, 2017 We tested whole-blood and plasma samples from immunocompetent patients who had had benign Zika virus infections and found that Zika virus RNA persisted in whole blood substantially longer than in plasma.
Jean Michel Mansuy +6 more
doaj +1 more source
Elevating microRNA-122 in blood improves outcomes after temporary middle cerebral artery occlusion in rats. [PDF]
, 2016 Because our recent studies have demonstrated that miR-122 decreased in whole blood of patients and in whole blood of rats following ischemic stroke, we tested whether elevating blood miR-122 would improve stroke outcomes in rats.
Ander, Bradley P +9 more
core +2 more sources
Quality of DNA extracted from formalin-fixed, paraffin-embedded canine tissues. [PDF]
, 2020 Veterinary pathology tissue banks are valuable resources for genetic studies. However, limited data exist as to whether quality DNA can be extracted from these tissues for use in canine genotyping studies.
Bannasch, Danika L +2 more
core +1 more source
FEBS Letters, EarlyView.Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek +15 more
wiley +1 more source
Global Journal of Transfusion Medicine, 2017 Background: Blood is precious and should be utilized judiciously with minimal wasting. By analyzing the data and the reasons for discard, the blood transfusion service can develop plans to evaluate causes that lead to discard of blood and its components ...
Ashishkumar Nathabhai Kanani +3 more
doaj +1 more source
Advancing Ischemic Stroke Diagnosis: A Novel Two-Stage Approach for Blood Clot Origin Identification [PDF]
arXiv, 2023 An innovative two-stage methodology for categorizing blood clot origins is presented in this paper, which is important for the diagnosis and treatment of ischemic stroke. First, a background classifier based on MobileNetV3 segments big whole-slide digital pathology images into numerous tiles to detect the presence of cellular material.
arxiv
Intracerebral Hemorrhage and Ischemic Stroke of Different Etiologies Have Distinct Alternatively Spliced mRNA Profiles in the Blood: a Pilot RNA-seq Study. [PDF]
, 2015 Whole transcriptome studies have used 3'-biased expression microarrays to study genes regulated in the blood of stroke patients. However, alternatively spliced messenger RNA isoforms have not been investigated for ischemic stroke or intracerebral ...
Ander, Bradley P +8 more
core +2 more sources
Autophagy in cancer and protein conformational disorders
FEBS Letters, EarlyView.Autophagy plays a crucial role in numerous biological processes, including protein and organelle quality control, development, immunity, and metabolism. Hence, dysregulation or mutations in autophagy‐related genes have been implicated in a wide range of human diseases.
Sergio Attanasio
wiley +1 more source
The comet assay in oral leucocytes: a non-invasive alternative in human studies?
Frontiers in Genetics, 2015 The comet assay is the method of choice for measuring DNA damage. In human studies mostly peripheral blood mononuclear cells (PBMC) are used to conduct this single cell gel electrophoresis (Azqueta and Collins, 2013) and also a method for whole blood was
Annemarie Grindel
doaj +1 more source