Results 71 to 80 of about 3,071,871 (357)

Release of cytokines in stored whole blood and red cell concentrate: Effect of leukoreduction

Asian Journal of Transfusion Science, 2015
Background: Storage time of blood components plays a major role in the accumulation of cytokines causing adverse transfusion reactions. Aims: The aim was to study the trend in the levels of interleukin-6 (IL-6), IL-8, tumor necrosis factor-alpha (TNF-α ...
Rinku Shukla, Tanvi Patel, Snehalata Gupte   +2 more
doaj   +1 more source

Therapeutic Drug Monitoring of Tacrolimus Based on Volumetric Absorptive Microsampling Technique (VAMS) in Renal Transplant Pediatric Recipients—LC-MS/MS Method Development, Hematocrit Effect Evaluation, and Clinical Application

Pharmaceutics, 2023
Tacrolimus (TAC) is post-transplant pharmacotherapy’s most widely used immunosuppressant. In routine clinical practice, frequent uncomfortable venipuncture is necessary for whole-blood (WB) collection to check trough TAC levels.
Arkadiusz Kocur, Dorota Marszałek, Jacek Rubik, Agnieszka Czajkowska, Tomasz Pawiński   +4 more
doaj   +1 more source

Data‐driven discovery of gene expression markers distinguishing pediatric acute lymphoblastic leukemia subtypes

Molecular Oncology, EarlyView.
This study investigates gene expression differences between two major pediatric acute lymphoblastic leukemia (ALL) subtypes, B‐cell precursor ALL, and T‐cell ALL, using a data‐driven approach consisting of biostatistics and machine learning methods. Following analysis of a discovery dataset, we find a set of 14 expression markers differentiating the ...
Mona Nourbakhsh, Nikola Tom, Anna Schrøder Lassen, Helene Brasch Lind Petersen, Ulrik Kristoffer Stoltze, Karin Wadt, Kjeld Schmiegelow, Matteo Tiberti, Elena Papaleo   +8 more
wiley   +1 more source

Construction of a system for isolation and purification of NK cells from whole blood donations

Zhongguo shuxue zazhi
[Objective] To explore the feasibility of using whole blood as a source of NK cells for allogeneic CAR NK cell therapy and activated NK cell reinfusion therapy, and initially construct a technical system for the separation and purification of NK cells ...
CAO Tengyu, LIN Huayu, ZHANG Xuanzhi, DUAN Cuimi, LIU Yi, XUE Xiaonan, SUN Liping, YU Yang   +7 more
doaj   +1 more source

Sensitive and specific detection of Trypanosoma cruzi DNA in clinical specimens using a multi-target real-time PCR approach [PDF]

, 2012
Background: The laboratory diagnosis of Chagas disease is challenging because the usefulness of different diagnostic tests will depend on the stage of the disease.
Aznar, Christine, da Silva, Alexandre J., Qvarnstrom, Yvonne, Schijman, Alejandro Gabriel, Steurer, Francis, Veron, Vincent   +5 more
core   +4 more sources

Genomics‐led approach to drug testing in models of undifferentiated pleomorphic sarcoma

Molecular Oncology, EarlyView.
GA text Genomic data from undifferentiated pleomorphic sarcoma patients and preclinical models were used to inform a targeted drug screen. Selected compounds were tested in 2D and 3D cultures of UPS cell lines. A combination of trametinib and infigratinib was synergistic in the majority of UPS cell lines tested, which was further confirmed in an ex ...
Piotr J. Manasterski, Molly R. Danks, John P. Thomson, Morwenna Muir, Martin Lee, John C. Dawson, Ana T. Amaral, Juan Diaz‐Martin, David S. Moura, Javier Martin‐Broto, Ali Alsaadi, Donald M. Salter, Ailsa J. Oswald, Graeme Grimes, Larry Hayward, Ted R. Hupp, Karen Sisley, Paul H. Huang, Neil O. Carragher, Valerie G. Brunton   +19 more
wiley   +1 more source

Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells [PDF]

, 2010
This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane “slot” filter. More than 90% viable cancer cells could be
Goldkorn, Amir, Lu, Bo, Tai, Yu-Chong, Xu, Tong, Zheng, Siyang   +4 more
core   +1 more source

Unraveling LINE‐1 retrotransposition in head and neck squamous cell carcinoma

Molecular Oncology, EarlyView.
The novel RetroTest method allows the detection of L1 activation in clinical samples with low DNA input, providing global L1 activity and the identification of the L1 source element. We applied RetroTest to a real‐world cohort of HNSCC patients where we reported an early L1 activation, with more than 60% of T1 patients showing L1 activity.
Jenifer Brea‐Iglesias, Ana Oitabén, Sonia Zumalave, Bernardo Rodriguez‐Martin, María Gallardo‐Gómez, Martín Santamarina, Ana Pequeño‐Valtierra, Laura Juaneda‐Magdalena, Ramón García‐Escudero, José Luis López‐Cedrún, Máximo Fraga, José M. C. Tubio, Mónica Martínez‐Fernández   +12 more
wiley   +1 more source

EMT‐associated bias in the Parsortix® system observed with pancreatic cancer cell lines

Molecular Oncology, EarlyView.
The Parsortix® system was tested for CTC enrichment using pancreatic cancer cell lines with different EMT phenotypes. Spike‐in experiments showed lower recovery of mesenchymal‐like cells. This was confirmed with an EMT‐inducible breast cancer cell line.
Nele Vandenbussche, Renske Imschoot, Béatrice Lintermans, Lode Denolf, Joachim Taminau, Charlotte Fieuws, Geert Berx, Kris Gevaert, Kathleen B. M. Claes   +8 more
wiley   +1 more source

PCR Targeting Plasmodium Mitochondrial Genome of DNA Extracted from Dried Blood on Filter Paper Compared to Whole Blood. [PDF]

, 2014
Monitoring mortality and morbidity attributable to malaria is paramount to achieve elimination of malaria. Diagnosis of malaria is challenging and PCR is a reliable method for identifying malaria with high sensitivity.
A Diallo, A Farnert, AM Dondorp, AP Phyo, B Morassin, Bjørn Blomberg, C Fancony, C Haanshuus, C Wongsrichanalai, CJ Murray, CV Plowe, E Bottius, GE Strom, Gro EA Strøm, H Reyburn, J Hwang, J Wooden, K Moll, LC Okell, LN Wangai, M Pritsch, Maulidi Fataki, N Valecha, NB Gadalla, Nina Langeland, RJ Pearce, S Ataei, S Chaorattanakawee, S Proux, S Shekalaghe, SA Al-Harthi, Sabrina Moyo, T Hanscheid, WHO, WHO   +34 more
core   +3 more sources