Results 71 to 80 of about 268,424 (252)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Into the wild—Exploring the life cycles of yeasts

Yeast, 2021
Nishant, K.T., Włoch-Salamon, Dominika, Wolfe, Kenneth H., Nobile, Clarissa J.   +3 more
openaire   +4 more sources

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

Exploration of wild yeasts isolated from marula fruit wine as suitable candidates for industrial craft beer brewing

European Journal of Horticultural Science
Exploration of the natural microbial diversity of indigenous fruits can lead to the discovery of promising candidate yeasts that bring diversity and unique characteristics to commercial brews compared with commercial strains.
Louis Ramoba, Paulina Rapeso, Annastasia Lekganyane, Matholo Mothapo, Kgabo Maganedi   +4 more
doaj   +1 more source

Knockout and transcriptome sequencing analysis of cdc50 gene in Saccharomyces cerevisiae cells [PDF]

Zhongguo niangzao
In order to explore the effect of cdc50 gene on the function of Saccharomyces cerevisiae cells, using PYM14 plasmid as the template, the cdc50 gene in wild type S.
LI Xinyu, TANG Xiuqin, LI Zihang, LIU Jia, WANG Qi, ZOU Wei
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Synphilin-1 enhances α-synuclein aggregation in yeast and contributes to cellular stress and cell death in a Sir2-dependent manner.

PLoS ONE, 2010
BackgroundParkinson's disease is characterized by the presence of cytoplasmic inclusions, known as Lewy bodies, containing both aggregated α-synuclein and its interaction partner, synphilin-1.
Sabrina Büttner, Charlotte Delay, Vanessa Franssens, Tine Bammens, Doris Ruli, Sandra Zaunschirm, Rita Machado de Oliveira, Tiago Fleming Outeiro, Frank Madeo, Luc Buée, Marie-Christine Galas, Joris Winderickx   +11 more
doaj   +1 more source

Advanced Materials for Biologics Delivery to Brain Tumors

Advanced Materials, EarlyView.
Material innovation is central to unlocking the therapeutic potential of biologics against many central nervous system diseases, including brain cancer. By engineering carriers with controlled transport, targeting, and release properties, advanced materials can overcome the blood–brain barrier and tumor microenvironment, improving the delivery of ...
Yuran Feng, Yuxue Cao, Maria Kavallaris, Taskeen Janjua, Amirali Popat   +4 more
wiley   +1 more source

Collagen‐Based Gut‐on‐Chip for In Vitro Modeling of Intestinal Barrier Function and Host‐Pathogen Interactions

Advanced Materials Technologies, EarlyView.
A collagen‐based gut‐on‐chip model replicates the intestinal extracellular matrix, supporting villi‐like structures, tight junctions, and mucin production. This biomimetic platform enables rapid epithelial differentiation and functional barrier formation.
Fernanda López García, Jacques Leng, Hugo de Oliveira, Pierre Nassoy, Karine Dementhon   +4 more
wiley   +1 more source