Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
[Evaluation of work capacity in diabetics].
J, Frenkel, S, Berber
openaire +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Building community-engaged research capacity through bidirectional collaboration: the community research consulting initiative model at NYU Langone health. [PDF]
Onakomaiya D +6 more
europepmc +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
The development and utility of frameworks designed to evaluate research capacity building initiatives in healthcare settings: a methodological review. [PDF]
King OA +8 more
europepmc +1 more source
Evaluation of Scaling Efforts in CGIAR: Technical Note
CGIAR has maintained a long-standing commitment to scaling the public benefits of its innovations and has recently reinforced this priority. This renewed emphasis has generated an increasing need for the systematic evaluation of scaling efforts.
CGIAR Independent Advisory and Evaluation Service
core
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Beyond the Audit Cycle: Using Quality Improvement Data to Inform Dental Commissioning. [PDF]
Biggadike A, Shirlaw P, Johnson J.
europepmc +1 more source
Terms of Reference: Country Evaluation Pilot Kenya
: These Terms of Reference outline the CGIAR country evaluation in Kenya. Piloting an independent process and performance country evaluation provides the CGIAR management and governance bodies, and key Kenyan partners with context-specific evaluative ...
CGIAR Independent Advisory and Evaluation Service
core

