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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
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Empathy for those working in other disciplines
BMJ, 2020Morgan’s article about his experience wearing the scrubs of a healthcare assistant for the day presents a refreshing perspective, bringing empathy into sharp focus and breaking down the hierarchical barriers engrained …
Matthew J, Kane, Henry M J, White
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The Work of Discipline, the Discipline of Work
symploke, 2006Whither knowledge that requires a "contract"? Is there in fact any other kind of knowledge, at least in academic life? What is the relation of this knowledge to paradigms or politics? How in fact is a paradigm different than a discipline; how is each to be distinguished from a profession; and what about politics amid these distinctions?
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Work Discipline and Work Motivation on Employee Performance
International Journal of Economics, Social Science, Entrepreneurship and Technology (IJESET), 2022This study aims to determine partially and simultaneously the effect of work discipline and work motivation on employee performance at office Kantor Badan Pengelolaan Keuangan dan Aset Daerah Kabupaten Langkat. The population used in this study amounted to 50 employees.
Muhammad Irfan Nasution +1 more
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2010
In the previous chapter, we considered those methodologies that might be seen to characterize the archaeology of the contemporary past. One of the issues raised there was the extent to which an archaeology of the contemporary past is defined by, and is even reliant upon, working with and across a series of different academic disciplines and areas of ...
Rodney Harrison, John Schofield
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In the previous chapter, we considered those methodologies that might be seen to characterize the archaeology of the contemporary past. One of the issues raised there was the extent to which an archaeology of the contemporary past is defined by, and is even reliant upon, working with and across a series of different academic disciplines and areas of ...
Rodney Harrison, John Schofield
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The Impact Work Discipline and the Work Environment on the Performance
Cross Current International Journal of Economics, Management and Media Studies, 2022The purpose of this study is to identify and describe the motivation, work discipline, and the work environment; to analyze the significance level of motivation, work discipline, and the work environment; and to partially analyze the significance level of motivation, work discipline, and the work environment on the performance of Indonesian Air Force ...
Indra Yanuar +2 more
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Work Discipline and Conditions of Work
1983The ethical approach to work has varied historically and still differs today in different countries. In some societies, work has been linked with religion, as symbolized by the medieval Christian maxim, ora et labora (‘pray and work’), where honest and hard work was considered to be conducive to salvation.
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