Results 111 to 120 of about 196,851 (322)

Quantitative Proteomics and CRISPR/Cas9 Editing Reveal UPR‐Mediated Control of Immunoglobulin Homeostasis in Hybridomas

Advanced Science, EarlyView.
BCR sequencing and subclone analysis correlated immunoglobulin (Ig) chain loss in dysfunctional hybridomas with disrupted monoclonal antibody homeostasis. Proteomics‐guided CRISPR/Cas9 editing revealed that the unfolded protein response (UPR) regulates aberrant Ig synthesis.
Rubing Zou, Xinying Lu, Ying Liu, Peiyu Yang, Shuo Sun, Yihua Liu, Yinyuan Mo, Guonian Zhu, Jae Seong Lee, Yirong Guo   +9 more
wiley   +1 more source

Varying levels of X chromosome coalescence in female somatic cells alters the balance of X-linked dosage compensation and is implicated in female-dominant systemic lupus erythematosus

Scientific Reports, 2019
The three-dimensional organization of the genome in mammalian interphase nuclei is intrinsically linked to the regulation of gene expression. Whole chromosome territories and their encoded gene loci occupy preferential positions within the nucleus that ...
Agnieszka I. Laskowski, Daniel S. Neems, Kyle Laster, Chelsee Strojny-Okyere, Ellen L. Rice, Iwona M. Konieczna, Jessica H. Voss, James M. Mathew, Joseph R. Leventhal, Rosalind Ramsey-Goldman, Erica D. Smith, Steven T. Kosak   +11 more
doaj   +1 more source

The Evolutionarily Conserved TPM1 Super‐Enhancer Drives Skeletal Muscle Regeneration via Mechanotransduction Signaling

Advanced Science, EarlyView.
By integrating biomechanical and epigenetic cues, the evolutionarily conserved TPM1 super‐enhancer drives myogenesis via TEAD4‐mediated chromatin looping. This mechanism produces species‐specific outputs (linear TPM1 mRNA in mice and CircTPM1 in bovine) that activate PI3K/AKT mechanotransduction and the MYH10/MYL3 axis to execute cytoskeletal ...
Ruimen Zhang, Wanyou Feng, Yanyan Yang, Yu Pan, Chaoxia Zou, Leyi Wang, Sanbao Zhang, Yimin Zhao, Yongmei Wu, Jinling Wang, Jianwei Zou, Kehe Cen, Yongwang Zhang, Han Huang, Yurong Xu, Li Zhong, Hailong Gong, Juanru Cheng, Jingyuan Liang, Zihua Zheng, Qinyang Jiang, Jingwei Wei, Hui Li, Minhui Liang, Deshun Shi, Sufang Yang, Yanfei Deng, Yingming Wei   +27 more
wiley   +1 more source

Dynamic Relocalization of Histone Macroh2a1 from Centrosomes to Inactive X Chromosomes during X Inactivation [PDF]

, 2000
Theodore P. Rasmussen, Mary-Ann Mastrangelo, Amir Eden, John R. Pehrson, Rudolf Jaenisch   +4 more
openalex   +1 more source

Material‐Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells

Advanced Science, EarlyView.
Tuning cell and cytoskeleton mechanics modulated nuclear shape and heterochromatin organization in ASCs. Distinct cytoskeletal architectures induced nuclear morphologies from oblate to prolate ellipsoids. Large elongated cells with a structured actin cap exhibited high nuclear strain, driving nuclear envelope deformation and heterochromatin ...
Carlo F. Natale, Luca Messina, Valeria Panzetta, Stefania Saporito, Costantino Menna, Maurizio Ventre, Paolo A. Netti   +6 more
wiley   +1 more source

PFOS Disrupts Oocyte Maturation and Early Embryonic Development via Ovarian FOXK1 O‐GlcNAcylation in Mice

Advanced Science, EarlyView.
Perfluorooctane sulfonate (PFOS) exposure disrupts oocyte maturation and early embryonic development. This study elucidates the mechanism by which enhanced O‐GlcNAcylation of FOXK1 underlies the PFOS‐induced reduction of progesterone levels in granulosa cells and the disturbance of follicular microenvironment.
Shuwen Han, Qin Yuan, Zhu Wu, Yaohui Fang, Hong Qian, Jiale Zhu, Yuchen Zhang, Ke Deng, Liangliang Su, Haibo Xu, Haotian Shu, Yiming Gong, Qiaoqiao Xu, Guizhen Du, Di Wu, Yun Fan, Chuncheng Lu   +16 more
wiley   +1 more source

X chromosome inactivation patterns in 45,X/46,XX mosaics [PDF]

, 2001
S. Uehara, Kosei Sato, Masaki Hashiyada, Y. Obara, Sachiko Matsuzaki, Masayuki Nata, K. Okamura   +6 more
openalex   +1 more source

Parallel Genome‐Wide CRISPR Screens Reveal SORL1 and ZFYVE19 as Sequential Host Determinants of Salmonella Infection

Advanced Science, EarlyView.
To distinguish how Salmonella invades cells vs how it survives long‐term, a parallel CRISPR screening platform is developed. This approach reveals the host proteins that the bacterium exploits at different stages of infection. The study identifies SORL1 as a novel host factor for invasion and demonstrates that blocking it with an antibody effectively ...
Sehee Yun, Seoyeon Kim, Seonggyu Kim, Minsoo Noh, Dae‐Kyum Kim, Eun‐Jin Lee, Hunsang Lee   +6 more
wiley   +1 more source

Genetic and Developmental Analysis of X-Inactivation in Interspecific Hybrid Mice Suggests a Role for the Y Chromosome in Placental Dysplasia [PDF]

, 2001
Myriam Hemberger, Haymo Kurz, Annie Orth, Sabine Otto, Angela Lüttges, Rosemary W. Elliott, András Nagy, Seong-Seng Tan, Patrick Tam, Ulrich Zechner, Reinald Fundele   +10 more
openalex   +1 more source

EGR Proteins Mediate Interferon‐Independent Anti‐HSV‐1 Responses Through Viral and Host Targets

Advanced Science, EarlyView.
Early antiviral responses are typically mediated by interferons. However, during HSV‐1 infection, host early growth response (Egr) genes, which are not interferon‐stimulated genes, are quickly induced by viral protein ICP0. EGR proteins, in turn, suppress viral lytic infection by activating viral latency‐associated (LAT) and host immune regulatory ...
Shuaishuai Wang, Fujun Hou, Xunuo Jin, Yuhang Xiang, Dongli Pan   +4 more
wiley   +1 more source