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Developmental Genetics in Xenopus tropicalis
2011The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with embryological and biochemical assays. Its early development closely resembles that of its well-understood tetraploid relative Xenopus laevis, from which techniques and reagents can be ...
Timothy J, Geach, Lyle B, Zimmerman
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2006
For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development ...
Jean-François L. Bodart +1 more
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For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development ...
Jean-François L. Bodart +1 more
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Obtaining Xenopus tropicalis Embryos by Natural Mating
Cold Spring Harbor Protocols, 2021Xenopus is a powerful model system for cell and developmental biology in part because frogs produce thousands of eggs and embryos year-round. Natural matings are a simple and common method to obtain embryos for injection or other experimental use or to raise to adulthood.
Maura Lane, Mustafa K. Khokha
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Generating Diploid Embryos from Xenopus tropicalis
2012A spectacular advantage of Xenopus tropicalis is the ease with which diploid embryos can be generated year round. By the simple administration of human chorionic gonadotropin, an investigator can generate many hundreds of synchronized embryos by in vitro fertilization or thousands of embryos from a mating pair.
Florencia, del Viso, Mustafa, Khokha
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Xenopus tropicalis (Gray, 1864) (Fig. 12C) MATERIAL EXAMINED. — Togo • 4 ♀; Agoté; MNHN-RA-2006.2172-2175. DESCRIPTION. — Medium to large frog (SVL 40-56 mm ♀), with flattened body. Snout rounded. Head very small, wider than long (HW 26-29% SVL; HL 21-26% SVL). Very small eyes and eyelids (EL 1.4-1.6 mm diameter); a very small subocular tentacle, about
Segniagbeto, Gabriel Hoinsoudé +4 more
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Segniagbeto, Gabriel Hoinsoudé +4 more
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Navigating the Xenopus tropicalis Genome
2012The frog Xenopus laevis has for more than 60 years served as a model system for the study of vertebrate embryogenesis, molecular and cell biology, and physiology. Recently, there has been great interest in the related species Xenopus tropicalis, in part because it is diploid, unlike the allotetraploid X.
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Cryopreservation of sperm ofXenopus laevis andXenopus tropicalis
genesis, 2005Now that transgenic strains of Xenopus laevis and X. tropicalis can be generated efficiently and with genomic sequence resources available for X. tropicalis, early amphibian development can be studied using integrated biochemical and genetic approaches.
Michael G, Sargent, Timothy J, Mohun
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Genetic and genomic prospects for Xenopus tropicalis research
Seminars in Cell & Developmental Biology, 2006Research using Xenopus laevis has made enormous contributions to our understanding of vertebrate development, control of the eukaryotic cell cycle and the cytoskeleton. One limitation, however, has been the lack of systematic genetic studies in Xenopus to complement molecular and cell biological investigations.
Samantha, Carruthers, Derek L, Stemple
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Cornea-lens transdifferentiation in the anuran, Xenopus tropicalis
Development Genes and Evolution, 2001Previously, the only anuran amphibian known to regenerate the lens of the eye was Xenopus laevis. This occurs during larval stages through transdifferentiation of the outer cornea epithelium under control of factors presumably secreted by the neural retina. This study demonstrates that a distantly related species, X.
J J, Henry, M B, Elkins
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Frog genetics: Xenopus tropicalis jumps into the future
Trends in Genetics, 1998. The two mainreasons for this are that the embryosdevelop externally and their relativelylarge size allows embryologists toperform microsurgery and manipu-late the embryos experimentally inways that are not as easy in other ver-tebrate embryos (e.g. mouse or zebra-fish).
Amaya, E. +2 more
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