Modelling human genetic disorders in Xenopus tropicalis [PDF]
Disease Models & MechanismsABSTRACT Recent progress in human disease genetics is leading to rapid advances in understanding pathobiological mechanisms. However, the sheer number of risk-conveying genetic variants being identified demands in vivo model systems that are amenable to functional analyses at scale. Here we provide a practical guide for using the diploid
Helen Rankin Willsey +5 more
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Revealing mitf functions and visualizing allografted tumor metastasis in colorless and immunodeficient Xenopus tropicalis [PDF]
Communications BiologyTransparent immunodeficient animal models not only enhance in vivo imaging investigations of visceral organ development but also facilitate in vivo tracking of transplanted tumor cells.
Rensen Ran +5 more
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Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis [PDF]
Cell & Bioscience, 2022 Background The true diploid frog, Xenopus tropicalis (X. tropicalis) is an excellent genetic model organism. To date, the CRISPR/Cas-mediated genome editing methods established in this species are mostly based on SpCas9 that requires the stringent NGG ...
Zhaoying Shi +5 more
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Cardiac regeneration in Xenopus tropicalis and Xenopus laevis: discrepancies and problems [PDF]
Cell & Bioscience, 2018 Two studies have recently focused on adult heart regeneration in Xenopus. While we reported on cardiac myogenic regeneration in Xenopus tropicalis after injury, Marshall and colleagues found no regeneration in an injured heart in Xenopus laevis. Here, we
Souqi Liao +5 more
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Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in Xenopus tropicalis snai2:eGFP line [PDF]
G3: Genes, Genomes, Genetics, 2022 Transgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization.
Jian Wang, Congyu Lu, Shuo Wei
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The Tmem16a chloride channel is required for mucin maturation after secretion from goblet-like cells in the Xenopus tropicalis tadpole skin [PDF]
Scientific ReportsThe TMEM16A chloride channel is proposed as a therapeutic target in cystic fibrosis, where activation of this ion channel might restore airway surface hydration and mitigate respiratory symptoms.
Eamon Dubaissi +10 more
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Pharmacological Modulation of Melanocortin 1 Receptor Signaling by Mrap Proteins in Xenopus tropicalis [PDF]
Frontiers in Endocrinology, 2022 The melanocortin system consists of five G protein–coupled receptors (MC1R-MC5R), the bidirectional endogenous ligands (MSH and Agouti families), and accessory proteins (MRAP1 and MRAP2).
Xiaolu Tai +8 more
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Comparison of TALEN scaffolds in Xenopus tropicalis [PDF]
Biology Open, 2013 Summary Transcription activator-like effector nucleases (TALENs) are facile and potent tools used to modify a gene of interest for targeted gene knockout.
Keisuke Nakajima, Yoshio Yaoita
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Liver-specific enhancers evolved from independent episodes of MITE domestication in Xenopus tropicalis [PDF]
Mobile DNATransposable elements (TEs) occupy a significant fraction of a wide variety of eukaryotic genomes and can be domesticated into functional sequences harbouring a coding or regulatory potential.
Japhet Rojas +6 more
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Correction to: Evolutionary divergence in tail regeneration between Xenopus laevis and Xenopus tropicalis [PDF]
Cell & Bioscience, 2021 An amendment to this paper has been published and can be accessed via the original article.
Shouhong Wang, Yun-Bo Shi
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