Results 11 to 20 of about 10,041 (187)

An epigenetic clock for Xenopus tropicalis. [PDF]

NPJ Aging
Abstract DNA methylation clocks have been widely used for accurate age prediction, but most studies have been carried out on mammals. Here we present an epigenetic clock for the aquatic frog Xenopus tropicalis, a widely used model organism in developmental biology and genomics.
Bennett R, Morselli M, Petrova K, Peshkin L, Pellegrini M.   +4 more
europepmc   +8 more sources

Xenopus tropicalis [PDF]

, 2020
Published as part of Ayoro, Halamoussa Joëlle, Segniagbeto, Gabriel Hoinsoudé, Hema, Emmanuel Midibahaye, Penner, Johannes, Oueda, Adama, Dubois, Alain, Rödel, Mark-Oliver, Kabré, Gustave Boureima & Ohler, Annemarie, 2020, List of amphibian species (Vertebrata, Tetrapoda) of Burkina Faso, pp.
Ayoro, Halamoussa Joëlle, Segniagbeto, Gabriel Hoinsoudé, Hema, Emmanuel Midibahaye, Penner, Johannes, Oueda, Adama, Dubois, Alain, Rödel, Mark-Oliver, Kabré, Gustave Boureima, Ohler, Annemarie   +8 more
core   +5 more sources

A genetic map of Xenopus tropicalis [PDF]

Developmental Biology, 2011
We present a genetic map for Xenopus tropicalis, consisting of 2886 Simple Sequence Length Polymorphism (SSLP) markers. Using a bioinformatics-based strategy, we identified unique SSLPs within the X. tropicalis genome. Scaffolds from X. tropicalis genome assembly 2.0 (JGI) were scanned for Simple Sequence Repeats (SSRs); unique SSRs were then tested ...
Wells, Dan E., Gutierrez, Laura, Xu, Zhenkang, Krylov, Vladimir, Macha, Jaroslav, Blankenburg, Kerstin P., Hitchens, Matthew, Bellot, Larry J., Spivey, Mary, Stemple, Derek L., Kowis, Andria, Ye, Yuan, Pasternak, Shiran, Owen, Jenetta, Tran, Thu, Slavikova, Renata, Tumova, Lucie, Tlapakova, Tereza, Seifertova, Eva, Scherer, Steven E., Sater, Amy K.   +20 more
openaire   +3 more sources

Modelling human genetic disorders in Xenopus tropicalis [PDF]

Disease Models & Mechanisms
ABSTRACT Recent progress in human disease genetics is leading to rapid advances in understanding pathobiological mechanisms. However, the sheer number of risk-conveying genetic variants being identified demands in vivo model systems that are amenable to functional analyses at scale. Here we provide a practical guide for using the diploid
Helen Rankin Willsey, Eleanor G. Seaby, Annie Godwin, Sarah Ennis, Matthew Guille, Robert M. Grainger   +5 more
doaj   +4 more sources

Cardiac regeneration in Xenopus tropicalis and Xenopus laevis: discrepancies and problems [PDF]

Cell & Bioscience, 2018
Two studies have recently focused on adult heart regeneration in Xenopus. While we reported on cardiac myogenic regeneration in Xenopus tropicalis after injury, Marshall and colleagues found no regeneration in an injured heart in Xenopus laevis. Here, we
Souqi Liao, Wenyan Dong, Hui Zhao, Ruijin Huang, Xufeng Qi, Dongqing Cai   +5 more
doaj   +3 more sources

Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in Xenopus tropicalis snai2:eGFP line [PDF]

G3: Genes, Genomes, Genetics, 2022
Transgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization.
Jian Wang, Congyu Lu, Shuo Wei
doaj   +2 more sources

Targeted integration of genes in Xenopus tropicalis [PDF]

genesis, 2017
AbstractWith the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination‐mediated targeted integration in ...
Shi, Zhaoying, Tian, Dandan, Xin, Huhu, Lian, Jingru, Guo, Xiaogang, Chen, Yonglong   +5 more
openaire   +4 more sources

The Tmem16a chloride channel is required for mucin maturation after secretion from goblet-like cells in the Xenopus tropicalis tadpole skin [PDF]

Scientific Reports
The TMEM16A chloride channel is proposed as a therapeutic target in cystic fibrosis, where activation of this ion channel might restore airway surface hydration and mitigate respiratory symptoms.
Eamon Dubaissi, Emma N. Hilton, Sarah Lilley, Richard Collins, Charlotte Holt, Peter March, Henry Danahay, Martin Gosling, Richard K Grencis, Ian S Roberts, David J Thornton   +10 more
doaj   +3 more sources

Cas9-Based Genome Editing in Xenopus tropicalis [PDF]

, 2014
Xenopus tropicalis has been developed as a model organism for developmental biology, providing a system offering both modern genetics and classical embryology. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) system for genome modification has provided an additional tool for Xenopus researchers to ...
Takuya, Nakayama, Ira L, Blitz, Margaret B, Fish, Akinleye O, Odeleye, Sumanth, Manohar, Ken W Y, Cho, Robert M, Grainger   +6 more
openaire   +5 more sources

Comparison of TALEN scaffolds in Xenopus tropicalis [PDF]

Biology Open, 2013
Summary Transcription activator-like effector nucleases (TALENs) are facile and potent tools used to modify a gene of interest for targeted gene knockout.
Keisuke Nakajima, Yoshio Yaoita
doaj   +3 more sources