Results 61 to 70 of about 22,476 (202)

Structural and thermodynamic analyses of a novel β‐1,2‐glucan binding mode in the ABC transporter solute‐binding protein Chy400_4166 from Chloroflexus aurantiacus

open access: yesThe FEBS Journal, EarlyView.
Cyclic β‐1,2‐glucan (CβG)‐associated solute‐binding protein of type I ABC transporter was originally identified in Chloroflexus aurantiacus, a phototrophic bacterium. Crystal structures in complex with CβGs revealed unique binding modes that are fundamentally distinct from that of the short β‐1,2‐glucooligosaccharides‐binding protein.
Kazuya Kato   +6 more
wiley   +1 more source

Fine-tuning xylan side chains for enhanced production of high-yield sugars, fine-shaped nanocrystals, and stable Pickering emulsions in Arabidopsis thaliana

open access: yesBiotechnology for Biofuels and Bioproducts
Arabinoxylans are complex polysaccharides found in the secondary cell walls of plants, and their substitution patterns of arabinose (Ara) and glucuronic acid (GlcA) significantly affect their properties for structural and bio-functional applications.
Sufang Li   +9 more
doaj   +1 more source

Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3

open access: yesBrazilian Archives of Biology and Technology, 2005
In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were ...
Seyis Isil, Aksoz Nilufer
doaj   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

open access: yesThe FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti   +8 more
wiley   +1 more source

Structural features of xylan dictate reactivity and functionalization potential for bio-based materials

open access: yesBiotechnology for Biofuels and Bioproducts
Background Plant-based materials have the potential to replace some petroleum-based products, offering compostability and biodegradability as critical advantages.
Mohammad Aghajohari   +2 more
doaj   +1 more source

Structural insights into xyloglucan recognition by an ABC transporter from a Gram‐positive, thermophilic bacterium

open access: yesThe FEBS Journal, EarlyView.
Athe_2052 is an ABC transporter substrate‐binding protein that recognizes xyloglucan heptasaccharide (XXXG), a soluble breakdown product of plant xyloglucan. The crystal structure of Athe_2052, together with binding assays and computational modeling, shows how XXXG is recognized by this sugar transporter.
Hansen Tjo   +6 more
wiley   +1 more source

Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications

open access: yesBiotechnology for Biofuels and Bioproducts
Background The pattern of xylan backbone substitution by 4-O-methylglucuronic acid (mGlcA) and O-acetyl groups generated during biosynthesis in the Golgi apparatus plays a key role in molecular interactions between secondary cell wall polymers in ...
Pramod Sivan   +8 more
doaj   +1 more source

Reassigning the role of a mesophilic xylan hydrolysing family GH43 β-xylosidase from Bacteroides ovatus, BoExXyl43A as exo-β-1,4-xylosidase

open access: yesCurrent Research in Biotechnology
The recombinant 40 kDa BoExXyl43A glycoside hydrolase family 43 (GH43) from bacterium Bacteroides ovatus exhibited highest specific activity (U/mg) against corn cob xylan (136.8), followed by Beechwood xylan (81.1), Carbosynth xylan (69.3), 4-O-D ...
Parmeshwar Vitthal Gavande   +4 more
doaj   +1 more source

Partial purification and characterization of xylanase produced by Penicillium expansum

open access: yesBrazilian Archives of Biology and Technology, 2006
An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity.
André Luiz de Souza Querido   +3 more
doaj   +1 more source

Ecological and genomic variation in ectomycorrhizal fungal exploration types

open access: yesNew Phytologist, EarlyView.
Summary Ectomycorrhizal fungi (EMF) produce mycelia with variable extension and complexity, which can be classified according to soil ‘exploration types’ (ETs). ETs have received attention as one of the few mycorrhizal trait frameworks, but without an empirical classification of ET functional diversity and environmental preferences, understanding and ...
Thomas M. Mansfield   +55 more
wiley   +1 more source

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