Results 201 to 210 of about 11,026 (229)

Crystallographic studies of the mechanism of xylose isomerase

Biochemistry, 1989
The mechanism of xylose isomerase (EC 5.3.1.5) has been studied with X-ray crystallography. Four refined crystal structures are reported at 3-A resolution: native enzyme, enzyme + glucose, enzyme + glucose + Mg2+, and enzyme + glucose + Mn2+. One of these structures (E.G.Mg) was determined in a crystal mounted in a flow cell.
Gerard Tiraby, Dagmar Ringe, Gregory A. Petsko, Gregory K. Farber, Arthur Glasfeld   +4 more
openaire   +3 more sources

Determination of the anomeric configuration of D-xylose with D-xylose isomerases

Carbohydrate Research, 1976
Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
Clement K. De Bruyne, Marc Claeyssens, Elisa Van Doorslaer, Hilda Kersters-Hilderson   +3 more
openaire   +3 more sources

Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium

Bioprocess and Biosystems Engineering, 2011
Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The
Gwon-Young Jeong, Kyung-Hwan Jeong, Byung Sam Ko, Seunghyun Choi, Jae-Gun Han, Il-Chul Kim, Yonggwan Won, Han-Bit Jang, Dongmin Kim, Hyeon-Yong Lee   +9 more
openaire   +3 more sources

Xylose isomerases from Thermotoga

2001
Publisher Summary Typically present in microorganisms that grow on xylose, xylose isomerise (XI) converts xylose to xylulose, which is then phosphorylated and enters the pentose-phosphate pathway. Because it also accepts glucose as substrate, XI is used extensively to isomerize glucose to fructose in the manufacture of high fructose corn syrup (HFCS).
J. G. Zeikus, Dinlaka Sriprapundh, Claire Vieille, Robert M. Kelly   +3 more
openaire   +3 more sources

Overproduction of d-xylose isomerase in Escherichia coli by cloning the d-xylose isomerase gene

Enzyme and Microbial Technology, 1985
Abstract The Escherichia coli d -xylose isomerase ( d -xylose ketol-isomerase, EC 5.3.1.5) gene , xyl A, has been cloned on various E. coli plasmids. However, it has been found that high levels of overproduction of the d -xylose isomerase, the protein product of the xyl A gene, cannot be accomplished by cloning the intact gene on high copy ...
Panagiotis E. Stevis, Nancy W. Y. Ho
openaire   +2 more sources

Hyperexpression of Escherichia coli Xylose Isomerase

Biotechnology Progress, 1986
AbstractThe xylose isomerase (xylA) structural gene was cloned under the control of the tac promoter and expressed in a xyl+ E. coli strain. Xylose isomerase accounted for approximately 28% of the total cell protein when this tac‐xylA fusion was induced with isopropylthio β‐D‐galactopyranoside.
Judy Ko, Steven R. Novak, Anthony J. Sinskey, Carl A. Batt, Elizabeth O'Neill   +4 more
openaire   +3 more sources

Overproduction of E. coli xylose isomerase

Biotechnology Letters, 1986
A promoterles DNA fragment containing theE. coli xylose isomerase gene and its ribosome binding site was ligated into a plasmid downstream from the strong λ PL promoter. The plasmid was then used to transformE. coli strains containing a temperature-sensitive λ repressor (cI857).
S. M. Lastick, V. Mackedonski, Karel Grohmann, M. Y. Tucker   +3 more
openaire   +2 more sources

Xylose Isomerase Catalysed Novel Hexose Epimerization

Biocatalysis and Biotransformation, 1999
Novel hexose substrates and new epimerization reactions catalysed by the industrially important D-xylose ketol-isomerase (E.C. 5. 3. 1. 5.) are described. Isomerization equilibrium betweer D-allose and D-psicose or D-sorbose and D-gulose is obtained starting from either of the substrates.
Pastinen, O., Schoemaker, H.E., Leisola, M.   +2 more
openaire   +4 more sources

Properties of genetically overproducedE. coli xylose isomerase [PDF]

Biotechnology Letters, 1988
Xylose isomerase was purified from a transformedE. coli strain (LE392-pRK248/pTXI-1) (Lastick et al., 1986) that overproduces the enzyme by induction of the strong lambda PL promotor. Kinetic data, N-terminal sequence analysis, SDS polyacrylamide gel electrophoresis, size exclusion chromatography and immunodiffusion were used to compare the ...
M. Y. Tucker, Melvin P. Tucker, S. M. Lastick, Karel Grohmann, Michael E. Himmel   +4 more
openaire   +1 more source

Metabolism of d-xylose in Schizosaccharomyces pombe cloned with a xylose isomerase gene

Applied Microbiology and Biotechnology, 1989
The Escherichia coli xylose isomerase gene was transformed into Schizosaccharomyces pombe for direct d-xylose utilization. In order to understand d-xylose metabolism and determine the limiting factors on d-xylose utilization by the transformed yeast, d-xylose transport, xylose isomerization, and xylulose phosphorylation were investigated.
Li Fu Chen, Err-Cheng Chan, P. P. Ueng
openaire   +2 more sources