Results 211 to 220 of about 69,355 (257)

Xylose isomerases from Thermotoga

2001
Publisher Summary Typically present in microorganisms that grow on xylose, xylose isomerise (XI) converts xylose to xylulose, which is then phosphorylated and enters the pentose-phosphate pathway. Because it also accepts glucose as substrate, XI is used extensively to isomerize glucose to fructose in the manufacture of high fructose corn syrup (HFCS).
C, Vieille, D, Sriprapundh, R M, Kelly, J G, Zeikus   +3 more
openaire   +2 more sources

Structure and mechanism of D-xylose isomerase

Faraday Discussions, 1992
The action of xylose isomerase depends on the presence of two divalent cations. Crystal structure analyses of the free enzyme, and of the enzyme bound to a variety of substrates and inhibitors, have provided models for a number of distinct intermediates along the reaction pathway.
D M, Blow, C A, Collyer, J D, Goldberg, O S, Smart   +3 more
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Mechanism of Action of D-Xylose Isomerase

Current Protein & Peptide Science, 2000
The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized. First we deal shortly with experimental methods applied to study the structure and function of this enzyme: enzyme kinetics, protein engineering, X-ray crystallography, nuclear magnetic and ...
B, Asbóth, G, Náray-Szabó
openaire   +2 more sources

Hyperexpression of Escherichia coli Xylose Isomerase

Biotechnology Progress, 1986
AbstractThe xylose isomerase (xylA) structural gene was cloned under the control of the tac promoter and expressed in a xyl+ E. coli strain. Xylose isomerase accounted for approximately 28% of the total cell protein when this tac‐xylA fusion was induced with isopropylthio β‐D‐galactopyranoside.
C A, Batt, E, O'Neill, S R, Novak, J, Ko, A, Sinskey   +4 more
openaire   +2 more sources

Overproduction of d-xylose isomerase in Escherichia coli by cloning the d-xylose isomerase gene

Enzyme and Microbial Technology, 1985
Abstract The Escherichia coli d -xylose isomerase ( d -xylose ketol-isomerase, EC 5.3.1.5) gene , xyl A, has been cloned on various E. coli plasmids. However, it has been found that high levels of overproduction of the d -xylose isomerase, the protein product of the xyl A gene, cannot be accomplished by cloning the intact gene on high copy ...
Panagiotis E. Stevis, Nancy W.Y. Ho
openaire   +1 more source

Immunoaffinity purification of glucose/xylose isomerase fromStreptomyces

Applied Biochemistry and Biotechnology, 1991
A procedure was developed to purify glucose/xylose isomerase from cell extract of Streptomyces sp. NCIM 2730 using immunoaffinity chromatography. High-titer polyclonal antibodies were raised in rabbit using electrophoretically homogeneous glucose/xylose isomerase as an antigen.
M, Ghatge, Y, Mawal, S, Gaikwad, V, Deshpande   +3 more
openaire   +2 more sources

Determination of the anomeric configuration of D-xylose with D-xylose isomerases

Carbohydrate Research, 1976
Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
H, Kersters-Hilderson, M, Claeyssens, E, van Doorslaer, C K, de Bruyne   +3 more
openaire   +2 more sources

Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production

Preparative Biochemistry & Biotechnology
Saccharomyces cerevisiae cannot assimilate xylose, second to glucose derived from lignocellulosic biomass. Here, the engineered S. cerevisiae strains INVSc-XI and INVSc-XI/XT were constructed using xylA and Xltr1p to co-utilize xylose and glucose ...
Mengtian Huang, Xinxin Cui, Peining Zhang, Zhuocheng Jin, Huanan Li, Jiashu Liu, Zhengbing Jiang   +6 more
semanticscholar   +1 more source

Overproduction of E. coli xylose isomerase

Biotechnology Letters, 1986
A promoterles DNA fragment containing theE. coli xylose isomerase gene and its ribosome binding site was ligated into a plasmid downstream from the strong λ PL promoter. The plasmid was then used to transformE. coli strains containing a temperature-sensitive λ repressor (cI857).
S. M. Lastick, M. Y. Tucker, V. Mackedonski, K. Grohmann   +3 more
openaire   +1 more source

Crystallographic studies of the mechanism of xylose isomerase

Biochemistry, 1989
The mechanism of xylose isomerase (EC 5.3.1.5) has been studied with X-ray crystallography. Four refined crystal structures are reported at 3-A resolution: native enzyme, enzyme + glucose, enzyme + glucose + Mg2+, and enzyme + glucose + Mn2+. One of these structures (E.G.Mg) was determined in a crystal mounted in a flow cell.
G K, Farber, A, Glasfeld, G, Tiraby, D, Ringe, G A, Petsko   +4 more
openaire   +2 more sources