Results 161 to 170 of about 25,231 (183)
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Oxidative d-xylose metabolism of Gluconobacter oxydans
Applied Microbiology and Biotechnology, 1988Gluconobacter oxydans subsp. suboxydans ATCC 621 oxidizes d-xylose to xylonic acid very efficiently, although it cannot grow on xylose as sole carbon source. The oxidation of xylose was found to be catalyzed by a membrane-bound xylose dehydrogenase. The xylono-γ-lactone formed in the oxidation reaction is subsequently hydrolyzed to xylonic acid by a γ ...
Viikari, Liisa, Buchert, Johanna
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Metabolic Control Analysis of Xylose Catabolism in Aspergillus
Biotechnology Progress, 2008A kinetic model for xylose catabolism in Aspergillus is proposed. From a thermodynamic analysis it was found that the intermediate xylitol will accumulate during xylose catabolism. Use of the kinetic model allowed metabolic control analysis (MCA) of the xylose catabolic pathway to be carried out, and flux control was shown to be dependent on the ...
Prathumpai, W. +6 more
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D-Xylose metabolism in Rhodosporidium toruloides
Biotechnology Letters, 1997Hofer et al. (Biochem. Biophys. Acta 1971. 252:1-12) presented circumstantial evidence that suggested that Rhodosporidium toruloides produced a xylose isomerase. We were unable to detect this activity in cell-free extracts of this yeast, however, xylose reductase and xylitol dehydrogenase activities were detected.
Christopher D. Skory +2 more
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Engineering yeasts for xylose metabolism
Current Opinion in Biotechnology, 2006Technologies for the production of alternative fuels are receiving increased attention owing to concerns over the rising cost of petrol and global warming. One such technology under development is the use of yeasts for the commercial fermentation of xylose to ethanol. Several approaches have been employed to engineer xylose metabolism.
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Xylose metabolism in genetic variants of Salmonella typhosa
Archives of Biochemistry and Biophysics, 1957Abstract Evidence has been presented which establishes steps in the route of xylose utilization in certain strains of S. typhosa . The failure of the negative strain to metabolize d -xylose stems from its inability to form the enzyme, xylose isomerase, and the enzyme, xylulokinase. The positive strain of S.
L.S. Baron, E.S. Kline
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Calculation of metabolic flow of xylose in Lactococcus lactis
Journal of Bioscience and Bioengineering, 2007A circuit diagram is proposed on the basis of an analysis of metabolic pathways of lactic acid bacteria, namely, a phosphoketolase pathway and a pentose phosphate/glycolic pathway. An augmented matrix was derived from carbon balances and stoichiometries from the circuit diagram, and solved by Gaussian elimination.
Michiko Owaki +2 more
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Metabolism of d-xylose in Schizosaccharomyces pombe cloned with a xylose isomerase gene
Applied Microbiology and Biotechnology, 1989The Escherichia coli xylose isomerase gene was transformed into Schizosaccharomyces pombe for direct d-xylose utilization. In order to understand d-xylose metabolism and determine the limiting factors on d-xylose utilization by the transformed yeast, d-xylose transport, xylose isomerization, and xylulose phosphorylation were investigated.
Li Fu Chen, Err-Cheng Chan, P. P. Ueng
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Xylose metabolism in Pachysolen tannophilus: purification and properties of xylose reductase
Canadian Journal of Microbiology, 1984Xylose reductase (xylitol: NADP oxidoreductase, EC 1.1.1.139) has been purified from D-xylose grown cells of the yeast Pachysolen tannophilus by application of DEAE-cellulose ion exchange chromatography, 2′,5′-ADP-Sepharose affinity chromatography, Biogel P200 gel filtration, and dextran blue Sepharose chromatography to approximately 95% homogeneity ...
Günther Ditzelmüller +3 more
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Metabolic pathway analysis of the xylose-metabolizing yeast protoplast fusant ZLYRHZ7
Journal of Bioscience and Bioengineering, 2017Xylose is the second major fermentable sugar present in hard woods and herbs (after d-glucose). Therefore, efficient conversion of xylose to ethanol is essential for the commercialization of lignocellulosic ethanol, which may provide an ideal alternative to fossil fuels in the future.
Jingping Ge +4 more
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Metabolic Engineering of Saccharomyces cerevisiae for Xylose Utilization
2001Metabolic engineering of Saccharomyces cerevisiae for ethanolic fermentation of xylose is summarized with emphasis on progress made during the last decade. Advances in xylose transport, initial xylose metabolism, selection of host strains, transformation and classical breeding techniques applied to industrial polyploid strains as well as modeling of ...
Leif J. Jönsson +5 more
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