Results 1 to 10 of about 7,576 (203)

Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinant Saccharomyces cerevisiae strains at high xylose concentrations [PDF]

Biotechnology for Biofuels, 2020
Background The need to restructure the world’s energy matrix based on fossil fuels and mitigate greenhouse gas emissions stimulated the development of new biobased technologies for renewable energy.
João Gabriel Ribeiro Bueno, Guilherme Borelli, Thamy Lívia Ribeiro Corrêa, Mateus Bernabe Fiamenghi, Juliana José, Murilo de Carvalho, Leandro Cristante de Oliveira, Gonçalo A. G. Pereira, Leandro Vieira dos Santos   +8 more
doaj   +7 more sources

New xylose transporters support the simultaneous consumption of glucose and xylose in Escherichia coli

mLife, 2022
Glucose and xylose are two major components of lignocellulose. Simultaneous consumption of glucose and xylose is critical for engineered microorganisms to produce fuels and chemicals from lignocellulosic biomass. Although many production limitations have
Xinna Zhu, Feiyu Fan, Huanna Qiu, Mengyao Shao, Di Li, Yong Yu, Changhao Bi, Xueli Zhang   +7 more
doaj   +3 more sources

Resurrection and characterization of ancestral xylose transporters enhance the capability of xylose uptake in the mixed sugar co-fermentation of Recombinant Saccharomyces cerevisiae [PDF]

Bioresources and Bioprocessing
Ancestral sequence reconstruction (ASR) offers a revolutionary approach to resurrect functional proteins, yet its potential in transporter engineering remains underexplored.
Peining Zhang, Zhaoqing He, Huanan Li, Zhengbing Jiang   +3 more
doaj   +2 more sources

Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production [PDF]

Synthetic and Systems Biotechnology
Xylose is a major component of lignocellulose, and it is crucial to enable microorganisms the capability of efficient xylose utilization for economical lignocellulosic biochemical production.
Jiyun Lou, Xia Wang, Runxia Li, Jun Yao, Junpeng Hu, Xianwu Qin, Mingjie Jin, Shihui Yang   +7 more
doaj   +2 more sources

Probing Carbon Utilization of Cordyceps militaris by Sugar Transportome and Protein Structural Analysis

Cells, 2020
Beyond comparative genomics, we identified 85 sugar transporter genes in Cordyceps militaris, clustering into nine subfamilies as sequence- and phylogenetic-based functional classification, presuming the versatile capability of the fungal growths on a ...
Kanokwadee Sirithep, Fei Xiao, Nachon Raethong, Yuhan Zhang, Kobkul Laoteng, Guang Hu, Wanwipa Vongsangnak   +6 more
doaj   +1 more source

Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei

Microbial Cell Factories, 2021
Background Lignocellulose biomass has been investigated as a feedstock for second generation biofuels and other value-added products. Some of the processes for biofuel production utilize cellulases and hemicellulases to convert the lignocellulosic ...
Sami Havukainen, Jonai Pujol-Giménez, Mari Valkonen, Matthias A. Hediger, Christopher P. Landowski   +4 more
doaj   +1 more source

Characterization of simultaneous uptake of xylose and glucose in Caldicellulosiruptor kronotskyensis for optimal hydrogen production

Biotechnology for Biofuels, 2021
Background Caldicellulosiruptor kronotskyensis has gained interest for its ability to grow on various lignocellulosic biomass. The aim of this study was to investigate the growth profiles of C. kronotskyensis in the presence of mixtures of glucose–xylose.
Thitiwut Vongkampang, Krishnan Sreenivas, Jonathan Engvall, Carl Grey, Ed W. J. van Niel   +4 more
doaj   +1 more source

Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast

Microbial Cell Factories, 2020
Background Xylose transport is one of the bottlenecks in the conversion of lignocellulosic biomass to ethanol. Xylose consumption by the wild-type strains of xylose-utilizing yeasts occurs once glucose is depleted resulting in a long fermentation process
Roksolana Vasylyshyn, Olena Kurylenko, Justyna Ruchala, Nadiya Shevchuk, Neringa Kuliesiene, Galina Khroustalyova, Alexander Rapoport, Rimantas Daugelavicius, Kostyantyn Dmytruk, Andriy Sibirny   +9 more
doaj   +1 more source

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

Nature Communications, 2020
XylE is a bacterial xylose transporter and homologue of human glucose transporters GLUTs 1-4. HDX-MS, mutagenesis and MD simulations suggest that protonation of a conserved aspartate triggers conformational transition from outward- to inward facing state
Ruyu Jia, Chloe Martens, Mrinal Shekhar, Shashank Pant, Grant A. Pellowe, Andy M. Lau, Heather E. Findlay, Nicola J. Harris, Emad Tajkhorshid, Paula J. Booth, Argyris Politis   +10 more
doaj   +1 more source

Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications

Frontiers in Bioengineering and Biotechnology, 2020
Lignocellulosic biomass yields after hydrolysis, besides the hexose D-glucose, D-xylose, and L-arabinose as main pentose sugars. In second generation bioethanol production utilizing the yeast Saccharomyces cerevisiae, it is critical that all three sugars
Jeroen G. Nijland, Arnold J. M. Driessen
doaj   +1 more source