Results 51 to 60 of about 6,974 (138)

Engineering heterologous enzyme secretion in Yarrowia lipolytica

Microbial Cell Factories, 2022
Background Eukaryotic cells are often preferred for the production of complex enzymes and biopharmaceuticals due to their ability to form post-translational modifications and inherent quality control system within the endoplasmic reticulum (ER).
Weigao Wang, Mark A. Blenner
doaj   +1 more source

Expanding the Genetic Toolkit of Pichia kudriavzevii E1 for Enhanced Mycoprotein Production

Microbial Biotechnology, Volume 19, Issue 9, September 2026.
A library of endogenous promoters with a 455‐fold activity range was developed using an established episomal expression vector in the Pichia kudriavzevii E1 strain. The promoter library was used to regulate genes associated with nitrogen assimilation and pyruvate metabolism to enhance mycoprotein production.
Yupeng Wang, Shenghan Gao, Chenchen Wu, Liangchen Guo, Peijie Han, Limin Wang, Justyna Ruchała, Bo Yu   +7 more
wiley   +1 more source

Safety evaluation of the food additive rebaudioside M produced via enzymatic conversion of purified steviol glycosides leaf extracts

EFSA Journal, Volume 24, Issue 9, September 2026.
Abstract The EFSA Panel on Food Additives and Flavourings (FAF Panel) provides a scientific opinion on the safety of a modified manufacturing process for the food additive enzymatically produced steviol glycosides (E 960c). The new process converts purified steviol glycosides extracted from Stevia rebaudiana leaves through enzymatic bioconversion ...
EFSA Panel on Food Additives and Flavourings (FAF), Laurence Castle, Monica Andreassen, Gabriele Aquilina, Maria Lourdes Bastos, Polly Boon, Biagio Fallico, Rex FitzGerald, Maria Jose Frutos Fernandez, Bettina Grasl‐Kraupp, Ursula Gundert‐Remy, Rainer Gürtler, Eric Houdeau, Marcin Kurek, Henriqueta Louro, Patricia Morales, Sabina Passamonti, José Manuel Barat Baviera, Gisela Degen, David Gott, Lieve Herman, Jean‐Charles Leblanc, Peter Moldeus, Ine Waalkens‐Berendsen, Detlef Wölfle, Consuelo Civitella, Samuele di Ciano, Simone Lunardi, Alessandra Tosato, Laura Ruggeri   +29 more
wiley   +1 more source

Microbiological Production of Citric and Isocitric Acids from Sunflower Oil

Food Technology and Biotechnology, 2008
The growth of wild type strain Yarrowia lipolytica VKM Y-2373 and its mutant Yarrowia lipolytica N 15 as well the biosynthesis of citric and isocitric acids on sunflower oil were studied.
Svetlana V. Kamzolova, Tatiana V. Finogenova, Igor G. Morgunov   +2 more
doaj  

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, Volume 101, Issue 4, Page 1662-1682, August 2026.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

2′‐FL's Depiction of the Human Physiological Function Landscape

Food Science &Nutrition, Volume 14, Issue 8, August 2026.
The figure summarizes the multifaceted physiological effects of 2′‐fucosyllactose (2′‐FL), a major human milk oligosaccharide. The schematic illustrates the central role of the gut‐centered regulatory axis, where 2′‐FL modulates gut microbiota composition, promotes the production of short‐chain fatty acids and microbial metabolites, enhances mucus ...
Yushan Xiao, Jiahao Li, Xiaofeng Li, Yan Dai   +3 more
wiley   +1 more source

Partial replacement of fish meal with

Aquaculture Reports
This study investigated the effects of dietary Yarrowia lipolytica as a partial fish meal substitute on growth performance and lipid-related responses in turbot ( Scophthalmus maximus ).
Chengnan Ding, Hengtai Tang, Zhirui Zhang, Feng He, Pingping Liu, Tianyang Cai   +5 more
doaj   +1 more source

Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry

Food Technology and Biotechnology, 2011
The possibility of using glycerol and glycerol-containing waste from biodiesel manufacture as a carbon and energy source for microbiological production of citric acid has been studied.
Svetlana V. Kamzolova, Alina R. Fatykhova, Emiliya G. Dedyukhina, Savas G. Anastassiadis, Nikolay P. Golovchenko, Igor G. Morgunov   +5 more
doaj  

A Heterologous Push–Pull Metabolic Engineering Strategy Synergistically Enhances Citric Acid Secretion in Yarrowia lipolytica

Fermentation
Citric acid is one of the most effective organic acids for rare earth bioleaching. Yarrowia lipolytica represents a promising chassis due to its inability to produce oxalic acid; however, the metabolic bottlenecks between citrate synthesis and ...
Meng Song, Huangfeng Qiu, Donghua Tan, Jingqi Liu, Yuting Liang, Yu Yang   +5 more
doaj   +1 more source

Cold-active extracellular lipase: Expression in Sf9 insect cells, purification, and catalysis

Biotechnology Reports, 2019
Cold-active lipases are gaining special attention nowadays as they are increasingly used in various industries such as fine chemical synthesis, food processing, and washer detergent.
Tang Li, Wenfa Zhang, Jianhua Hao, Mi Sun, Sheng-Xiang Lin   +4 more
doaj   +1 more source