Results 31 to 40 of about 82,783 (260)

Preparation and Physicochemical Properties Analysis of Yeast β-Glucan Nanoparticles

Shipin gongye ke-ji
Yeast β-glucan was a multifunctional polysaccharide, but it was insoluble in water and present particles. To broaden its applicability, the yeast β-glucan nanoparticles were prepared by ball milling pulverization using yeast cells of Saccharomyces ...
Xiaohong HOU, Guoqun ZHAO, Jinlong LIU
doaj   +1 more source

Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast

Nature Communications, 2021
The molecular events underlying the assembly and maturation of the early pre-60S particles during eukaryotic ribosome synthesis are not well understood. Here, the authors combine yeast genetics and biochemical experiments to characterise the functions of
Mariam Jaafar, Julia Contreras, Carine Dominique, Sara Martín-Villanueva, Régine Capeyrou, Patrice Vitali, Olga Rodríguez-Galán, Carmen Velasco, Odile Humbert, Nicholas J. Watkins, Eduardo Villalobo, Katherine E. Bohnsack, Markus T. Bohnsack, Yves Henry, Raghida Abou Merhi, Jesús de la Cruz, Anthony K. Henras   +16 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Yeast processing bodies and stress granules: self-assembly ribonucleoprotein particles

Microbial Cell Factories, 2011
Processing bodies (PBs) and stress granules (SGs) are two highly conserved cytoplasmic ribonucleoprotein foci that contain translationally repressed mRNAs together with proteins from the mRNA metabolism.
Díez Juana, Giménez-Barcons Mireia
doaj   +1 more source

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

FEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez, Mikael Oliveberg, Peter Brzezinski   +2 more
wiley   +1 more source

Targeting Nanodiamonds to the Nucleus in Yeast Cells

Nanomaterials, 2020
Nanodiamonds are widely used for drug delivery, labelling or nanoscale sensing. For all these applications it is highly beneficial to have control over the intracellular location of the particles.
Aryan Morita, Thamir Hamoh, Alina Sigaeva, Neda Norouzi, Andreas Nagl, Kiran J. van der Laan, Emily P. P. Evans, Romana Schirhagl   +7 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Highly stable Saccharomyces cerevisiae L-BC capsids with versatile packing potential

Frontiers in Bioengineering and Biotechnology
Virus-like particles (VLPs) are promising nanoscaffolds in development of vaccines and nanodelivery systems. Along with efficient production in various expression systems, they also offer extensive functionalization options.
Enrika Celitan, Ramunė Stanevičienė, Elena Servienė, Saulius Serva   +3 more
doaj   +1 more source