Results 11 to 20 of about 3,619 (189)

YidC and SecYEG form a heterotetrameric protein translocation channel [PDF]

Scientific Reports, 2017
The heterotrimeric SecYEG complex cooperates with YidC to facilitate membrane protein insertion by an unknown mechanism. Here we show that YidC contacts the interior of the SecY channel resulting in a ligand-activated and voltage-dependent complex with ...
Ilie Sachelaru, Lukas Winter, Denis G. Knyazev, Mirjam Zimmermann, Andreas Vogt, Roland Kuttner, Nicole Ollinger, Christine Siligan, Peter Pohl, Hans-Georg Koch   +9 more
doaj   +4 more sources

Key contributions of a glycolipid to membrane protein integration [PDF]

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
Regulation of membrane protein integration involves molecular devices such as Sec-translocons or the insertase YidC. We have identified an integration-promoting factor in the inner membrane of Escherichia coli called membrane protein integrase (MPIase ...
Keiko SHIMAMOTO, Kohki FUJIKAWA, Tsukiho OSAWA, Shoko MORI, Kaoru NOMURA, Ken-ichi NISHIYAMA   +5 more
doaj   +2 more sources

Structural basis of Sec-independent membrane protein insertion by YidC [PDF]

Nature, 2014
[プレスリリース]バイオサイエンス研究科膜分子複合機能学研究室の塚崎智也准教授らの研究グループが、タンパク質を細胞膜に組み込むメカニズムを解明しました（2014/04/17）Newly synthesized membrane proteins must be accurately inserted into the membrane, folded and assembled for proper functioning. The protein YidC inserts its substrates
Arisaka, Fumio, Chiba, Shinobu, Dohmae, Naoshi, Furukawa, Arata, Hirata, Kunio, Ishitani, Ryuichiro, Ito, Koreaki, Kumazaki, Kaoru, Maturana, Andrés D., Mori, Hiroyuki, Mori, Takaharu, Nakada-Nakura, Yoshiko, Nishiyama, Ken-ichi, Nureki, Osamu, Sugano, Yasunori, Sugita, Yuji, Takemoto, Mizuki, Tanaka, Yoshiki, Tsukazaki, Tomoya   +18 more
core   +3 more sources

Off-target structural insights: ArnA and AcrB in bacterial membrane-protein cryo-EM analysis. [PDF]

Acta Crystallogr D Struct Biol
This study highlights that cryo‐EM can reveal structural information beyond the intended target, and that overexpression of membrane proteins involved in quality control may lead to the co‐purification of proteins such as ArnA and AcrB.Membrane‐protein quality control in Escherichia coli involves coordinated actions of the AAA+ protease FtsH, the ...
Caliseki M, Borucu U, Yadav SKN, Schaffitzel C, Kabasakal BV.   +4 more
europepmc   +3 more sources

Combined 1H-Detected solid-state NMR spectroscopy and electron cryotomography to study membrane proteins across resolutions in native environments [PDF]

, 2017
Membrane proteins remain challenging targets for structural biology, despite much effort, as their native environment is heterogeneous and complex. Most methods rely on detergents to extract membrane proteins from their native environment, but this ...
Andreas, Andrew, Arnold J.M. Driessen, Asami, Atherton, Baker, Baker, Baker, Beck, C. Alistair Siebert, Cady, Chaikeeratisak, Chang, Cuniasse, Demers, Demers, Etzkorn, Folkers, Frank, Fricke, Goddard, Gradmann, Grant, Gremer, Han, Harris, Hartmann, Heise, Herzfeld, Heumann, Hong, Hullo, Jacso, Jeanine de Keyzer, Jensen, Kanehisa, Kaplan, Kaplan, Kaplan, Kaushik, Kay Grünewald, Kedrov, Kedrov, Kremer, Kumazaki, Lindsay A. Baker, Mance, Marc Baldus, Mastronarde, McMullan, Medeiros-Silva, Mi, Miao, Nans, Nishikawa, Park, Pascale Schellenberger, Perilla, Pettersen, Renault, Renault, Renault, Rigort, Rogawski, Rutkowska, Sborgi, Schagger, Schellenberger, Schindelin, Scotti, Sharma, Sinnige, Suzuki, Sychev, Taheri-Araghi, Tessa Sinnige, Umbarger, van der Cruijsen, van der Laan, Vuister, Wang, Ward, Ward, Ward, Wei, Weingarth, Wu, Wu, Yamamoto, Zhou   +89 more
core   +14 more sources

Downregulation of yidC in Escherichia coli by antisense RNA expression results in sensitization to antibacterial essential oils eugenol and carvacrol. [PDF]

PLoS ONE, 2013
BACKGROUND: The rising drug resistance in pathogenic bacteria and inefficiency of current antibiotics to meet clinical requirements has augmented the need to establish new and innovative approaches for antibacterial drug discovery involving ...
Supriya Deepak Patil, Rajnikant Sharma, Santosh Srivastava, Naveen Kumar Navani, Ranjana Pathania   +4 more
doaj   +1 more source

YidC-mediated membrane insertion [PDF]

FEMS Microbiology Letters, 2018
The most simple membrane protein insertion catalyst known so far is the universal YidC/Oxa/Alb insertase that is composed of a single multi-spanning protein present in archaea, bacteria and in eukaryotic organelles. In bacteria, YidC is known to integrate small membrane proteins on its own and more complex proteins in conjunction with the SecYEG ...
Dorothee, Kiefer, Andreas, Kuhn
openaire   +2 more sources

Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins

Scientific Reports, 2021
The membrane insertase YidC inserts newly synthesized proteins by its hydrophobic slide consisting of the two transmembrane (TM) segments TM3 and TM5. Mutations in this part of the protein affect the insertion of the client proteins.
Anja Steudle, Dirk Spann, Eva Pross, Sri Karthika Shanmugam, Ross E. Dalbey, Andreas Kuhn   +5 more
doaj   +1 more source

YidC/Alb3/Oxa1 Family of Insertases [PDF]

Journal of Biological Chemistry, 2015
The YidC/Alb3/Oxa1 family functions in the insertion and folding of proteins in the bacterial cytoplasmic membrane, the chloroplast thylakoid membrane, and the mitochondrial inner membrane. All members share a conserved region composed of five transmembrane regions.
Seth W, Hennon, Raunak, Soman, Lu, Zhu, Ross E, Dalbey   +3 more
openaire   +2 more sources

Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC. [PDF]

PLoS ONE, 2013
Membrane protein translocation and insertion is a central issue in biology. Here we focus on a minimal system, the membrane insertase YidC of Escherichia coli that inserts small proteins into the cytoplasmic membrane. In a reconstituted system individual
Sophie Winterfeld, Stefan Ernst, Michael Börsch, Uwe Gerken, Andreas Kuhn   +4 more
doaj   +1 more source