Monitoring the binding and insertion of a single transmembrane protein by an insertase
Nature Communications, 2021 The insertion and folding nascent or fully synthesized polypeptides into membranes is assisted by insertases. Here, the authors use a range of biophysical approaches to provide molecular details of how the transmembrane insertase YidC facilitates the ...
Pawel R. Laskowski +6 more
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The evolution of YidC/Oxa/Alb3 family in the three domains of life: a phylogenomic analysis
BMC Evolutionary Biology, 2009 Background YidC/Oxa/Alb3 family includes a group of conserved translocases that are essential for protein insertion into inner membranes of bacteria and mitochondria, and thylakoid membranes of chloroplasts.
Tian Hai-Feng +2 more
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Mapping of the saccharomyces cerevisiae oxa1-mitochondrial ribosome interface and identification of MrpL40, a ribosomal protein in close proximity to oxal and critical for oxidative phosphorylation complex assembly [PDF]
, 2009 The Oxa1 protein plays a central role in facilitating the cotranslational insertion of the nascent polypeptide chains into the mitochondrial inner membrane. Mitochondrially encoded proteins are synthesized on matrix-localized ribosomes which are tethered
Jia, Lixia +2 more
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A YidC-like Protein in the Archaeal Plasma Membrane [PDF]
Structure, 2015 Cells possess specialized machinery to direct the insertion of membrane proteins into the lipid bilayer. In bacteria, the essential protein YidC inserts certain proteins into the plasma membrane, and eukaryotic orthologs are present in the mitochondrial inner membrane and the chloroplast thylakoid membrane.
Borowska, Marta T. +4 more
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Signal Recognition Particle: An Essential Protein-Targeting Machine [PDF]
, 2013 The signal recognition particle (SRP) and its receptor compose a universally conserved and essential cellular machinery that couples the synthesis of nascent proteins to their proper membrane localization. The past decade has witnessed an explosion in
Akopian, David +3 more
core +1 more source
Bacterial Signal Peptides- Navigating the Journey of Proteins
Frontiers in Physiology, 2022 In 1971, Blobel proposed the first statement of the Signal Hypothesis which suggested that proteins have amino-terminal sequences that dictate their export and localization in the cell.
Sharbani Kaushik +2 more
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Elucidating the Native Architecture of the YidC: Ribosome Complex
Journal of Molecular Biology, 2013 Membrane protein biogenesis in bacteria occurs via dedicated molecular systems SecYEG and YidC that function independently and in cooperation. YidC belongs to the universally conserved Oxa1/Alb3/YidC family of membrane insertases and is believed to associate with translating ribosomes at the membrane surface.
Kedrov, Alexej +5 more
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Frontiers in Molecular Biosciences, 2021 The spatial and temporal coordination of protein transport is an essential cornerstone of the bacterial adaptation to different environmental conditions.
Julia Oswald +7 more
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YidC assists the stepwise and stochastic folding of membrane proteins [PDF]
Nature Chemical Biology, 2016 How chaperones, insertases and translocases facilitate insertion and folding of complex cytoplasmic proteins into cellular membranes is not fully understood. Here we utilize single-molecule force spectroscopy to observe YidC, a transmembrane chaperone and insertase, sculpting the folding trajectory of the polytopic α-helical membrane protein lactose ...
Tetiana Serdiuk +5 more
openaire +4 more sources
Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis
eLife, 2020 The outer-membrane of Gram-negative bacteria is critical for surface adhesion, pathogenicity, antibiotic resistance and survival. The major constituent – hydrophobic β-barrel Outer-Membrane Proteins (OMPs) – are first secreted across the inner-membrane ...
Sara Alvira +11 more
doaj +1 more source