Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Syntheses of 2,2'-dihalo-1,1'-binaphthyl compounds via palladium-catalysed 1,2-halo shift-cyclisation coupling reaction of α-hydroxyl haloalkynes. [PDF]
Nat CommunJi X, Li Y, Yu WJ, Wu W, Ni SF, Jiang H.
europepmc +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
The Promise of Synthetic Biology for Redesigning Plant Architecture. [PDF]
Int J Mol SciRoychoudhry S, Dos Santos GD, Lloyd JPB.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Sustainable Valorization of Banana Peel Agro-Waste in the Form of Citric Acid by <i>Aspergillus niger</i> ATCC 10535 Using Response Surface Methodology. [PDF]
ACS OmegaAbraha SK +9 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Development of green purification process for piperazine-type drugs: a case study of quetiapine process development. [PDF]
RSC AdvHuang W, Wang Y, Liu H, Li H, Yin Q.
europepmc +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Advances in Functional Genomics and Biotechnology for Enhancing Therapeutic Potential of Medicinal Plants. [PDF]
Int J Mol SciZaman W, Park S.
europepmc +1 more source