Results 81 to 90 of about 1,733,424 (331)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Strong modulation of optical properties in black phosphorus through strain-engineered rippling

, 2016
Controlling the bandgap through local-strain engineering is an exciting avenue for tailoring optoelectronic materials. Two-dimensional crystals are particularly suited for this purpose because they can withstand unprecedented non-homogeneous deformations
Agraït, Nicolás, Castellanos-Gomez, Andres, Guinea, Francisco, Molina-Mendoza, Aday, Parente, Vincenzo, Quereda, Jorge, Roldán, Rafael, Rubio-Bollinger, Gabino, San-José, Pablo, Vaquero-Garzon, Luis   +9 more
core   +2 more sources

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Assessment of Simple Engineering Approaches and Poultry Manure for Soil Erosion Control Under Maize Cultivation in the Tropics

Air, Soil and Water Research
Monitoring soil erosion is crucial for soil conservation policies, especially in tropical regions that are prone to water erosion. A 2-year field study was conducted to assess the impact of simple engineering approaches and poultry manure application on ...
Olanrewaju Hameed Ologunde, Sodiq Dimeji Akanni, Abdullahi Bamidele Olayemi, Mutiu Abolanle Busari   +3 more
doaj   +1 more source

Recent advances in keratinase production via protein engineering, breeding, and fermentation

Advanced Agrochem
The gene editing and synthetic biological tools have led to the implementation of diverse metabolic engineering approaches to enhance the production of specific enzymes. Microbial keratinases can convert keratin wastes into valuable compounds for mankind.
Ali Raza Ishaq, Zheng Zhang, Penghui He, Min Xiong, Shouwen Chen   +4 more
doaj   +1 more source

Web-based visualisation of head pose and facial expressions changes: monitoring human activity using depth data

, 2016
Despite significant recent advances in the field of head pose estimation and facial expression recognition, raising the cognitive level when analysing human activity presents serious challenges to current concepts.
Kalliatakis, Grigorios, Triantafyllidis, Georgios, Vidakis, Nikolaos   +2 more
core   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Statistics-based model for prediction of chemical biosynthesis yield from Saccharomyces cerevisiae

Microbial Cell Factories, 2011
Background The robustness of Saccharomyces cerevisiae in facilitating industrial-scale production of ethanol extends its utilization as a platform to synthesize other metabolites. Metabolic engineering strategies, typically via pathway overexpression and
Leonard Effendi, Xiao Yi, Varman Arul M, Tang Yinjie J   +3 more
doaj   +1 more source