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Fuchsin fluorescence in Mycobacterium tuberculosis: The Ziehl–Neelsen stain in a new light

Journal of Microbiological Methods, 2008
Tuberculosis (TB) is often diagnosed by observation of reddish pink fuchsin-stained Mycobacterium tuberculosis (MTB) in Ziehl-Neelsen (Z-N) stained smears by transmitted light microscopy. MTB too faintly stained with fuchsin to be seen by transmitted light may be detected by their green-excited orange-red fluorescence; this finding may be clinically ...
Howard M Shapiro, Thomas Hänscheid
exaly   +3 more sources

Notes on the ziehl-neelsen staining of sputum

Tubercle, 1974
Abstract When the incidence of tuberculosis is low, it is possible to save time and labour by making films of concentrates of sputum prepared for culture, 8 per double slide. Each positive is checked by a repeat film on the remainder of the original specimen. A tilting rack is described which makes staining easier and more efficient.
openaire   +2 more sources

Bulk staining of sputum by the Ziehl-Neelsen method

Tubercle, 1984
A trial of the Ziehl-Neelsen staining method employing bulk decolorization, rinsing and counter staining yielded reliable results. Of 110 patients who proved to be positive by direct microscopy, cross-contamination could not be proved in any. The advantage of the mass method is its economy.
openaire   +2 more sources

On the use of the Ziehl-Neelsen Technique for Specific Identification of Schistosome Eggs

Journal of Helminthology, 1972
By means of the standard Ziehl-Neelsen staining technique for eggs in tissue sections S. intercalatum can be differentiated from its sibling species S. haematobium, S. mattheei and S. bovis, since only S. intercalatum egg shells stain red.This technique could be of great value in Africa in clinical diagnosis and in determining the geographical limits ...
R L, Muller, M G, Taylor
openaire   +2 more sources

Detection of Mycobacterium Tuberculosis in Ziehl-Neelsen Sputum Smear Images

2018 11th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics (CISP-BMEI), 2018
Tuberculosis is one of the top 10 causes of death worldwide. In order to reduce the workload of doctors and the probability of human error, this paper presents an automatic detection algorithm for Mycobacterium tuberculosis in Ziehl-Neelsen Sputum Smear Images. The algorithm uses color feature and three morphological characters, which are aspect ratio,
Sineng Yan   +5 more
openaire   +1 more source

Colour image enhancement for Ziehl-Neelsen slide images

2008 International Conference on Electronic Design, 2008
The recognition of tuberculosis (TB) bacilli base on a color image processing is one of the most challenging tasks in image processing. The problems increase if the captured images are blurred or have low contrast. This paper proposes partial contrast and saturation technique methods to enhance color images of Ziehl-Neelsen stained smear for the ...
A.S.W. Wahab   +7 more
openaire   +1 more source

Evaluating the performance of basic fuchsin for the Ziehl-Neelsen stain.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 2009
An investigation of commercially available basic fuchsin (BF) dye powders used for the detection of acid-fast bacilli (AFB).To determine whether single, or multiple, assays may predict the performance of BF in the Ziehl-Neelsen (ZN) method.The composition and staining properties of six BF dye samples were assessed using continuous recording ...
C, Gordon, A, Van Deun, R, Lumb
openaire   +2 more sources

A modified Ziehl-Neelsen stain for mycobacteria.

Medical laboratory sciences, 1993
For the preliminary detection of mycobacteria in routine pathology specimens heavy reliance is placed on staining methods. Difficulties encountered with a cold-staining method, which had possibly gone un-noticed for several years despite a quality control check programme, are described. An alternative to the 'classical' Ziehl-Neelsen stain is described,
openaire   +1 more source

Ziehl-Neelsen Staining

2016
Neeta Patwardhan   +2 more
openaire   +2 more sources

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