A novel trophectoderm biopsy technique for all blastocyst stages
Purpose This study was conducted to assess the effectiveness of a new trophectoderm (TE) biopsy method that does not require prior opening of the zona pellucida at the blastocyst stage.
Yamato Mizobe +8 more
doaj +1 more source
Fractal analysis of zona pellucida thickness: bending energy as a predictive window on day 2 embryo fate [PDF]
OBJECTIVE: The quality of the embryo is crucial for the outcome of in vitro fertilization; however, the ability to accurately distinguish embryos with the highest reproductive potential from others is not entirely reliable.
M. Bezerra Espinola +7 more
doaj +1 more source
Construction of fluorescent human zona pellucida fusion protein expression vectors and expression in CHO cells [PDF]
Objective: To construct expression vectors of the fluorescent fusion proteins of all four human zona pellucida (ZP) genes respectively, and explore their expression in Chinese hamster ovary(CHO) cells and oocytes in vitro.
WANG Mingyi, ZHU Yan
doaj +1 more source
Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding [PDF]
Fertilization depends on successful binding of the spermatozoa to the zona pellucida of the oocyte. Glycodelin-A inhibits spermatozoa-zona pellucida binding.
Chiu, PCN +8 more
core +1 more source
Pre-implantation mouse embryos cultured In vitro under different oxygen concentrations show altered ultrastructures [PDF]
Assisted Reproductive Technologies routinely utilize different culture media and oxygen (O2) concentrations to culture human embryos. Overall, embryos cultured under physiological O2 tension (5%) have improved development compared to embryos cultured ...
Antonouli, S +6 more
core +1 more source
ZONA PELLUCIDA GLYCOPROTEINS [PDF]
All mammalian eggs are surrounded by a relatively thick extracellular coat, the zona pellucida, that plays vital roles during oogenesis, fertilization, and preimplantation development. The mouse zona pellucida consists of three glycoproteins that are synthesized solely by growing oocytes and assemble into long fibrils that constitute a matrix.
openaire +3 more sources
Background In human and rodents, sperm-zona pellucida binding is mediated by a sperm surface Galactosyltransferase that recognizes N-Acetylglucosamine residues on a glycoprotein ZPC. In large domestic mammals, the role of these molecules remains unclear:
Magistrini Michèle +5 more
doaj +1 more source
Zona pellucida-induced acrosome reaction in human spermatozoa is potentiated by glycodelin-A via down-regulation of extracellular signal-regulated kinases and up-regulation of zona pellucida-induced calcium influx [PDF]
Background Glycodelin-A interacts with spermatozoa before fertilization, but its role in modulating sperm functions is not known. Zona pellucida-induced acrosome reaction is crucial to fertilization and its dysfunction is a cause of male infertility.
Chiu, PCN +10 more
core +1 more source
Space asymmetry directs preferential sperm entry in the absence of polarity in the mouse oocyte. [PDF]
Knowledge about the mechanism that establishes embryonic polarity is fundamental in understanding mammalian development. In re-addressing several controversial claims, we recently proposed a model in which mouse embryonic polarity is not specified until ...
Nami Motosugi +4 more
doaj +1 more source
Assembly of the Inner Perivitelline Layer, a Homo log of the Mammalian Zona Pellucida: An Immunohistochemical and Ultrastructural Study [PDF]
The avian inner perivitelline layer (IPVL), a homologous structure to the mammalian zona pellucida, is deposited between the granulosa cells and the oocyte cell membrane during folliculogenesis. The glycoprotein meshwork of the IPVL forms a 3-dimensional
Bausek N. +37 more
core +1 more source

