Results 11 to 20 of about 14,154 (183)

Construction of fluorescent human zona pellucida fusion protein expression vectors and expression in CHO cells [PDF]

open access: yesZhenduanxue lilun yu shijian, 2021
Objective: To construct expression vectors of the fluorescent fusion proteins of all four human zona pellucida (ZP) genes respectively, and explore their expression in Chinese hamster ovary(CHO) cells and oocytes in vitro.
WANG Mingyi, ZHU Yan
doaj   +1 more source

ZONA PELLUCIDA GLYCOPROTEINS [PDF]

open access: yesAnnual Review of Biochemistry, 1988
All mammalian eggs are surrounded by a relatively thick extracellular coat, the zona pellucida, that plays vital roles during oogenesis, fertilization, and preimplantation development. The mouse zona pellucida consists of three glycoproteins that are synthesized solely by growing oocytes and assemble into long fibrils that constitute a matrix.
openaire   +3 more sources

The involvement of beta-1,4-galactosyltransferase and N-acetylglucosamine residues in fertilization has been lost in the horse

open access: yesReproductive Biology and Endocrinology, 2008
Background In human and rodents, sperm-zona pellucida binding is mediated by a sperm surface Galactosyltransferase that recognizes N-Acetylglucosamine residues on a glycoprotein ZPC. In large domestic mammals, the role of these molecules remains unclear:
Magistrini Michèle   +5 more
doaj   +1 more source

Artificial mineralized shell encapsulates mammalian oocytes

open access: yesMaterials Letters: X, 2021
Biomineralization refers to the process of functional minerals biosynthesis in organisms. This study introduced artificial mineralization operations on human oocytes and performed micromorphological and material studies.
Jing Fan   +4 more
doaj   +1 more source

Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes

open access: yesActa Veterinaria Scandinavica, 2007
Background Zona pellucida binding assays (ZBAs) have proven useful in determining the fertilising ability of spermatozoa in several species. Most ZBAs use fresh or salt-stored oocytes collected from fresh ovaries but because ovaries are not easy to ...
Holst Bodil   +2 more
doaj   +1 more source

Space asymmetry directs preferential sperm entry in the absence of polarity in the mouse oocyte. [PDF]

open access: yesPLoS Biology, 2006
Knowledge about the mechanism that establishes embryonic polarity is fundamental in understanding mammalian development. In re-addressing several controversial claims, we recently proposed a model in which mouse embryonic polarity is not specified until ...
Nami Motosugi   +4 more
doaj   +1 more source

The effect of chemical treatment of the sheep embryo zona pellucida on the ability of blastocysts to hatch after vitrification and warming

open access: yesVeterinary Medicine and Science, 2022
Background The embryo release from the zona pellucida is of prerequisites of successful implantation. Objectives Regarding the negative impact of embryo cryopreservation on the blastocysts hatchability, the aim of the present study was to investigate the
Fatemeh‐Sadat Mousavi   +4 more
doaj   +1 more source

Morphocytometric Evaluation of Equine Oocytes Following In Vitro Maturation

open access: yesBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine, 2023
In the current study, 930 equine oocytes were collected, 765 (82.25%) of which were considered viable for in vitro maturation. All selected oocytes were matured for 27 h at 38.50 0C in an atmosphere of 5% CO2 in humidified air.
Hussam ARYAN   +2 more
doaj   +1 more source

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts

open access: yesThe Journal of Reproduction and Development, 2019
The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is
Fuminori TANIHARA   +8 more
doaj   +1 more source

Oocyte surface in four teleost fish species postspawning and fertilization

open access: yesBrazilian Archives of Biology and Technology, 1998
Cytological and cytochemical studies were carried out to investigate the surface characteristics of oocytes of four teleost species from the São Francisco river.
Elizete Rizzo   +3 more
doaj   +1 more source

Home - About - Disclaimer - Privacy