Results 11 to 20 of about 14,154 (183)
Construction of fluorescent human zona pellucida fusion protein expression vectors and expression in CHO cells [PDF]
Objective: To construct expression vectors of the fluorescent fusion proteins of all four human zona pellucida (ZP) genes respectively, and explore their expression in Chinese hamster ovary(CHO) cells and oocytes in vitro.
WANG Mingyi, ZHU Yan
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ZONA PELLUCIDA GLYCOPROTEINS [PDF]
All mammalian eggs are surrounded by a relatively thick extracellular coat, the zona pellucida, that plays vital roles during oogenesis, fertilization, and preimplantation development. The mouse zona pellucida consists of three glycoproteins that are synthesized solely by growing oocytes and assemble into long fibrils that constitute a matrix.
openaire +3 more sources
Background In human and rodents, sperm-zona pellucida binding is mediated by a sperm surface Galactosyltransferase that recognizes N-Acetylglucosamine residues on a glycoprotein ZPC. In large domestic mammals, the role of these molecules remains unclear:
Magistrini Michèle +5 more
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Artificial mineralized shell encapsulates mammalian oocytes
Biomineralization refers to the process of functional minerals biosynthesis in organisms. This study introduced artificial mineralization operations on human oocytes and performed micromorphological and material studies.
Jing Fan +4 more
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Background Zona pellucida binding assays (ZBAs) have proven useful in determining the fertilising ability of spermatozoa in several species. Most ZBAs use fresh or salt-stored oocytes collected from fresh ovaries but because ovaries are not easy to ...
Holst Bodil +2 more
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Space asymmetry directs preferential sperm entry in the absence of polarity in the mouse oocyte. [PDF]
Knowledge about the mechanism that establishes embryonic polarity is fundamental in understanding mammalian development. In re-addressing several controversial claims, we recently proposed a model in which mouse embryonic polarity is not specified until ...
Nami Motosugi +4 more
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Background The embryo release from the zona pellucida is of prerequisites of successful implantation. Objectives Regarding the negative impact of embryo cryopreservation on the blastocysts hatchability, the aim of the present study was to investigate the
Fatemeh‐Sadat Mousavi +4 more
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Morphocytometric Evaluation of Equine Oocytes Following In Vitro Maturation
In the current study, 930 equine oocytes were collected, 765 (82.25%) of which were considered viable for in vitro maturation. All selected oocytes were matured for 27 h at 38.50 0C in an atmosphere of 5% CO2 in humidified air.
Hussam ARYAN +2 more
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The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts
The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is
Fuminori TANIHARA +8 more
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Oocyte surface in four teleost fish species postspawning and fertilization
Cytological and cytochemical studies were carried out to investigate the surface characteristics of oocytes of four teleost species from the São Francisco river.
Elizete Rizzo +3 more
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