Results 21 to 30 of about 14,154 (183)
Availability of empty zona pellucida for generating embryonic chimeras. [PDF]
PLoS ONE, 2014 In the present study we used an empty zona pellucida derived from hatched blastocysts as an alternative source for embryo aggregation and compared results with the conventional microwell method.
Chi-Hun Park +7 more
doaj +1 more source
Antigenicity of the Human Zona Pellucida [PDF]
Biology of Reproduction, 1977 Antigenicity of the human zona pellucida was investigated. Rabbit antiserum against saline homogenates of human ovary yielded a minimum of 9 precipitin bands when reacted against human ovary in agar-gel double diffusion analyses. The same antiserum also cross-reacted in varying degrees when tested against 21 other human tissues and fluids. Anti-human-
openaire +2 more sources
Sperm binding to the pig zona pellucida and inhibition of binding by solubilized components of the zona pellucida [PDF]
Reproduction, 1989 Summary. An assay to determine the binding of pig spermatozoa to the zona pellucida (ZP) of pig oocytes was developed using conditions compatible with in-vitro fertilization of pig eggs and with pig sperm penetration of zona-free hamster ova. These conditions were used to define which of the pig oocyte ZP components were involved in sperm binding by a ...
T, Berger +3 more
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CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
Bio-Protocol, 2019 Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA.
Naoaki Mizuno +5 more
doaj +1 more source
The effect of laser-assisted incubation on bovine in vitro embryos
Frontiers in Veterinary ScienceIntroductionThis paper aimed to enhance the hatching of bovine in vitro produced embryos by exploring their optimal LAH perforation/thinning pattern.MethodsOvaries were col-lected from an abattoir to obtain cumulus–oocyte complexes.
Wei Zhang +13 more
doaj +1 more source
Middle East Fertility Society Journal, 2018 Changes of oocyte quality and decreasing of ovarian follicle reserve in advanced age are the major challenges in infertility treatments. One factor associated with aging is the thickness of zona pellucida, which has adverse relation with embryo score ...
Masoomeh Mohammadzadeh +3 more
doaj +1 more source
Two candidate genes for recurrent pregnancy loss and infertility: Could ZP3 and UPK3B give us new diagnostic and therapeutic approach? [PDF]
BioDiscovery, 2017 Introduction: Chromosomal indels are relatively common cytogenetic abnormalities. Nonetheless, clinical outcomes depend on the location, size and genes in deletion or duplication regions.
Fatma Sılan +5 more
doaj +3 more sources
Immunoelectrophoretic analysis of the porcine zona pellucida [PDF]
Reproduction, 1983 Summary. Antigens of the porcine zona pellucida were evaluated by 2-dimensional and line immunoelectrophoretic techniques. Zona antigen preparations studied included heat-solubilized isolated zonae pellucidae (SIZP), a purified 60 000 Mr glycoprotein (PPZA, purified pig zona antigen), and two fractions of this 60 000 Mr zona component
A G, Sacco, E C, Yurewicz, S, Zhang
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Advanced Science, EarlyView.SDF‐1 levels decline significantly with maternal aging. Exogenous supplementation restores meiotic spindle morphology, chromosomal alignment, and mitochondrial function while reducing oxidative stress in aged oocytes. Mechanistically, SDF‐1 enhances autophagic activity to clear accumulated stress granules, thereby rescuing fertilization competence and ...
Rui Long +12 more
wiley +1 more source
Advanced Science, EarlyView.Oocytes play crucial roles in maintaining ovarian fate in adult gonochoristic fish. Targeted oocyte ablation with aromatase inhibition triggers the activation of surviving germline stem cells and the transdifferentiation of somatic cells from granoulosa/theca to Sertoli/Leydig lineages, inducing a complete and functional female‐to‐male sex reversal ...
Yang Yang +6 more
wiley +1 more source