Zona pellucida removal by acid Tyrode's solution affects pre- and post-implantation development and gene expression in mouse embryos(dagger) [PDF]
Zona pellucida removal by acid Tyrode's solution affects the inner cell mass/trophectoderm differentiation; a lower fetal rate, higher placental weight, and a total of 473 differentially expressed genes during post-implantation development in mice.
Tengda Huang +11 more
core +1 more source
Background Zona pellucida binding assays (ZBAs) have proven useful in determining the fertilising ability of spermatozoa in several species. Most ZBAs use fresh or salt-stored oocytes collected from fresh ovaries but because ovaries are not easy to ...
Holst Bodil +2 more
doaj +1 more source
Laser microdissection as a new approach to prefertilization genetic diagnosis [PDF]
The genetic status of oocytes can be determined by polar body (PB) analysis. Following PB extraction, a genetic evaluation is performed. As each PB contains the complementary genetic material of the oocyte, PB analysis reveals information about its ...
Schütze, Karin +2 more
core +1 more source
Space asymmetry directs preferential sperm entry in the absence of polarity in the mouse oocyte. [PDF]
Knowledge about the mechanism that establishes embryonic polarity is fundamental in understanding mammalian development. In re-addressing several controversial claims, we recently proposed a model in which mouse embryonic polarity is not specified until ...
Nami Motosugi +4 more
doaj +1 more source
Zona pellucida-induced acrosome reaction in human spermatozoa is potentiated by glycodelin-A via down-regulation of extracellular signal-regulated kinases and up-regulation of zona pellucida-induced calcium influx [PDF]
Background Glycodelin-A interacts with spermatozoa before fertilization, but its role in modulating sperm functions is not known. Zona pellucida-induced acrosome reaction is crucial to fertilization and its dysfunction is a cause of male infertility.
Satish K. Gupta +32 more
core +1 more source
Das Zusammenspiel der Proteasen Akrosin und Ovastacin bei der Härtung der Zona pellucida [PDF]
Die Zona pellucida umgibt die Eizellen von Säugetieren und ist in der Maus aus den Zona pellucida Proteinen 1-3 aufgebaut. Diese Proteine vermitteln vor der Befruchtung den ersten Kontakt von Spermium und Eizelle. Nach der Befruchtung verhindert die Zona
Kuske, Michael
core +1 more source
Background The embryo release from the zona pellucida is of prerequisites of successful implantation. Objectives Regarding the negative impact of embryo cryopreservation on the blastocysts hatchability, the aim of the present study was to investigate the
Fatemeh‐Sadat Mousavi +4 more
doaj +1 more source
Morphocytometric Evaluation of Equine Oocytes Following In Vitro Maturation
In the current study, 930 equine oocytes were collected, 765 (82.25%) of which were considered viable for in vitro maturation. All selected oocytes were matured for 27 h at 38.50 0C in an atmosphere of 5% CO2 in humidified air.
Hussam ARYAN +2 more
doaj +1 more source
Thinner zona pellucida in HSP90B1 deficient oocytes. [PDF]
(A) Representative images of wild-type (WT) and mutant (MT, obtained from Zp3-cre; Hsp90b1flox/flox females) ovulated oocytes showing that zona pellucida is thinner in mutant oocytes.
Christophe Audouard (356035) +4 more
core +1 more source
Cracking the egg: increased complexity in the zona pellucida [PDF]
A functional zona pellucida is critical for both fertilization and the early stages of embryo development. Recent data from genomic and proteomic studies have questioned our simplistic view of the zona as being composed of three proteins whose functions ...
Barratt, Christopher +14 more
core +1 more source

