Results 31 to 40 of about 17,404 (221)

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts

The Journal of Reproduction and Development, 2019
The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is
Fuminori TANIHARA, Maki HIRATA, Shigeki MORIKAWA, Nhien Thi NGUYEN, Quynh Anh LE, Takayuki HIRANO, Yoshiyuki FUKUMI, Toshiaki ABE, Takeshige OTOI   +8 more
doaj   +1 more source

Expression of Tas1 taste receptors in mammalian spermatozoa [PDF]

, 2012
Background: During their transit through the female genital tract, sperm have to recognize and discriminate numerous chemical compounds. However, our current knowledge of the molecular identity of appropriate chemosensory receptor proteins in sperm is ...
Ulrich Boehm (180339), de Angelis Martin Hrabé, Breit, A., Dorke Meyer, Andreas Breit (180329), Hrabě de Angelis, M., Widmayer Patricia, Wolfgang Meyerhof, Boekhoff Ingrid, Boehm, Ulrich, Martin Hrabé de Angelis, Meyer, Dorke, Ulrich Boehm, Dorke Meyer (180311), Sandra Huebner, Gudermann, T., Boehm, U., Borth, Heike, Borth Heike, Meyerhof, Wolfgang, Susan Marschall, Huebner Sandra, Voigt, Anja, Meyerhof, W., Hrabé de Angelis, Martin, Huebner, S., Sandra Huebner (180323), Thomas Gudermann (77426), Huebner, Sandra, Heike Borth, Borth, H., Susan Marschall (180334), Meyerhof Wolfgang, Ingrid Boekhoff, Ingrid Boekhoff (180345), Heike Borth (180317), Andreas Breit, Meyer Dorke, Boekhoff, I., Voigt Anja, Thomas Gudermann, Anja Voigt, Voigt, A., Gudermann Thomas, Breit Andreas, Boehm Ulrich, Marschall Susan, Marschall, Susan, Widmayer, Patricia, Widmayer, P., Wolfgang Meyerhof (7851), Patricia Widmayer, Martin Hrabé de Angelis (10007), Patricia Widmayer (180314), Breit, Andreas, Boekhoff, Ingrid, Gudermann, Thomas, Anja Voigt (173059), Marschall, S., Meyer, D.   +59 more
core   +1 more source

Oocyte surface in four teleost fish species postspawning and fertilization

Brazilian Archives of Biology and Technology, 1998
Cytological and cytochemical studies were carried out to investigate the surface characteristics of oocytes of four teleost species from the São Francisco river.
Elizete Rizzo, Thais F.C. Moura, Yoshimi Sato, Nilo Bazzoli   +3 more
doaj   +1 more source

Defining bioassay conditions to evaluate sperm/zona interaction: Inhibition of zona binding mediated by solubilized human zona pellucida [PDF]

, 1996
Objective: Our goal was to study the influence of solubilized human zonae pellucidae on zona binding potential and acrosome reaction. Materials and Methods: Zona pellucida (ZP) solutions were prepared by dissolving zona in acidic buffer, NaH2PO4 (pH 2.5),
Habenicht U.-F., Henkel R., Kaskar K., Franken D.R.   +3 more
core   +1 more source

002. PIG SPERM EGG INTERACTION AND FORMATION OF A ZONA PELLUCIDA BINDING COMPLEX [PDF]

, 2010
In order to achieve fertilization sperm cells first need to successfully interact with the zona pellucida. Before reaching the zona pellucida the sperm cell undergoes extensive remodeling both in the male and female genital tract.
B. M. Gadella
core   +1 more source

Sperm binding to the pig zona pellucida and inhibition of binding by solubilized components of the zona pellucida [PDF]

Reproduction, 1989
Summary. An assay to determine the binding of pig spermatozoa to the zona pellucida (ZP) of pig oocytes was developed using conditions compatible with in-vitro fertilization of pig eggs and with pig sperm penetration of zona-free hamster ova. These conditions were used to define which of the pig oocyte ZP components were involved in sperm binding by a ...
T, Berger, A, Davis, N J, Wardrip, J L, Hedrick   +3 more
openaire   +2 more sources

CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice

Bio-Protocol, 2019
Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA.
Naoaki Mizuno, Eiji Mizutani, Hideyuki Sato, Mariko Kasai, Hiromitsu Nakauchi, Tomoyuki Yamaguchi   +5 more
doaj   +1 more source

Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding [PDF]

, 2007
Fertilization depends on successful binding of the spermatozoa to the zona pellucida of the oocyte. Glycodelin-A inhibits spermatozoa-zona pellucida binding.
Chiu, PCN, Ho, PC, Seppala, M, Koistinen, R, Chung, MK, Ng, EHY, Lee, KF, Yeung, WSB, Koistinen, H   +8 more
core   +1 more source

Influential effect of age on oocyte morphometry, fertilization rate and embryo development following IVF in mice

Middle East Fertility Society Journal, 2018
Changes of oocyte quality and decreasing of ovarian follicle reserve in advanced age are the major challenges in infertility treatments. One factor associated with aging is the thickness of zona pellucida, which has adverse relation with embryo score ...
Masoomeh Mohammadzadeh, Farzaneh Fesahat, Arezoo Khoradmehr, Mohammad Ali Khalili   +3 more
doaj   +1 more source

Two candidate genes for recurrent pregnancy loss and infertility: Could ZP3 and UPK3B give us new diagnostic and therapeutic approach? [PDF]

BioDiscovery, 2017
Introduction: Chromosomal indels are relatively common cytogenetic abnormalities. Nonetheless, clinical outcomes depend on the location, size and genes in deletion or duplication regions.
Fatma Sılan, Baris Paksoy, Taner Karakaya, Onur Yildiz, Mine Urfali, Ozturk Ozdemir   +5 more
doaj   +3 more sources