The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts
The Journal of Reproduction and Development, 2019 The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is
Fuminori TANIHARA +8 more
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El sistema reproductor femenino de Zearaja chilensis (Guichenot, 1848) (Chondrichthyes, Rajidae): Gametogénesis y validación microscópica de los criterios de madurez [PDF]
, 2015 Los condrictios son extremadamente susceptibles a la explotación pesquera debido, entre otras cosas, a sus adaptaciones reproductivas. Los conocimientos básicos de sus parámetros reproductivos son esenciales, ya sea desde el punto de vista ecológico como
Di Giacomo, Edgardo Ernesto +2 more
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Oocyte surface in four teleost fish species postspawning and fertilization
Brazilian Archives of Biology and Technology, 1998 Cytological and cytochemical studies were carried out to investigate the surface characteristics of oocytes of four teleost species from the São Francisco river.
Elizete Rizzo +3 more
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Structure of the ovaries of the Nimba otter shrew, Micropotamogale lamottei, and the Madagascar hedgehog tenrec, Echinops telfairi [PDF]
, 2005 The otter shrews are members of the subfamily Potamogalinae within the family Tenrecidae. No description of the ovaries of any member of this subfamily has been published previously.
A.C. Enders +18 more
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Involvement of sperm acetylated histones and the nuclear isoform of Glutathione peroxidase 4 in fertilization [PDF]
, 2017 We previously demonstrated that the nuclear form of Glutathione peroxidase 4 (nGPx4) has a peculiar distribution in sperm head, being localized to nuclear matrix and acrosome and that sperm lacking nGPx4 are more prone to decondensation in vitro. In this
Boitani, Carla +8 more
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Sperm binding to the pig zona pellucida and inhibition of binding by solubilized components of the zona pellucida [PDF]
Reproduction, 1989 Summary. An assay to determine the binding of pig spermatozoa to the zona pellucida (ZP) of pig oocytes was developed using conditions compatible with in-vitro fertilization of pig eggs and with pig sperm penetration of zona-free hamster ova. These conditions were used to define which of the pig oocyte ZP components were involved in sperm binding by a ...
T, Berger +3 more
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Two candidate genes for recurrent pregnancy loss and infertility: Could ZP3 and UPK3B give us new diagnostic and therapeutic approach? [PDF]
BioDiscovery, 2017 Introduction: Chromosomal indels are relatively common cytogenetic abnormalities. Nonetheless, clinical outcomes depend on the location, size and genes in deletion or duplication regions.
Fatma Sılan +5 more
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Middle East Fertility Society Journal, 2018 Changes of oocyte quality and decreasing of ovarian follicle reserve in advanced age are the major challenges in infertility treatments. One factor associated with aging is the thickness of zona pellucida, which has adverse relation with embryo score ...
Masoomeh Mohammadzadeh +3 more
doaj +1 more source
Immunoelectrophoretic analysis of the porcine zona pellucida [PDF]
Reproduction, 1983 Summary. Antigens of the porcine zona pellucida were evaluated by 2-dimensional and line immunoelectrophoretic techniques. Zona antigen preparations studied included heat-solubilized isolated zonae pellucidae (SIZP), a purified 60 000 Mr glycoprotein (PPZA, purified pig zona antigen), and two fractions of this 60 000 Mr zona component
A G, Sacco, E C, Yurewicz, S, Zhang
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CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
Bio-Protocol, 2019 Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA.
Naoaki Mizuno +5 more
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