Optimizing the zymogram of exogenous proteases in broiler diets by in vitro simulated gastrointestinal digestion and response surface methodology [PDF]
Scientific ReportsLow protein digestibility causes intestinal flora imbalance, wet litter, and nitrogen pollution. The present study aimed to explore the optimal zymogram of exogenous acid protease (ACP), neutral protease (NEP), alkaline protease (ALP), and keratinase ...
Shijie Liu, Liu Shengli, Dai Qiuzhong
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Frontiers in Microbiology, 2017 The rumen bacterium Cellulosilyticum ruminicola H1 efficiently hydrolyzes cellulose. To gain insights into the regulatory mechanisms of cellulase synthesis, comparative transcriptome analysis was conducted for cultures grown on 2% filter paper, 0.5 and 0.
Xiuzhu Dong, Nana Shao, Hongcan Liu
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Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells [PDF]
BMC BiotechnologyThree-dimensional spheroids are more representative of tumors than cell-cultured monolayers. As in tumors, gradients of oxygen, nutrients and wastes are found in spheroid cultures but not in classical cultured monolayers.
Sandra Majo +7 more
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Detection of Proteolytic Activity by Fluorescent Zymogram In-Gel Assays [PDF]
BioTechniques, 2000 Proteases are involved in the regulation of many biological functions. This study describes a novel method for detecting protease activity by fluorescent zymogram in-gel protease assays, using SDS polyacrylamide gels copolymerized with a peptide-MCA (4 ...
Sukkid Yasothornsrikul, Vivian Y.H. Hook
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A thin layer chromatographic comparison of raw and soluble starch hydrolysis patterns of some α-amylases from Bacillus sp. isolated in Serbia [PDF]
Journal of the Serbian Chemical Society, 2014 Several natural isolates of Bacillus strains namely 5B, 12B, 16B, 18 and 24B were grown on two different temperatures in submerged fermentation for the raw-starch-digesting a-amylases production. All strains except Bacillus sp.
Gligorijević Nikola +5 more
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Zymography: developing of the enzyme soil activity visualization method
Бюллетень Почвенного института им. В.В. Докучаева, 2023 The enzymes produced by the soil biota are a key link in the regulation of biochemical processes. The soil enzyme activity can be visualized with zymography, a method based on using fluorescent substrates and obtaining two-dimensional images (zymograms).
M. V. Timofeeva +4 more
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Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae [PDF]
Archives of Biological Sciences, 2008 Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae.
Božić Nataša +5 more
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Animals, 2022 This experiment aimed to explore the zymogram of endo-xylanase (EX) and debranching enzymes (arabinofuranosidase [EA] and ferulic acid esterase [EF]) supplemented in the corn–soybean meal-based diet of broilers.
Wei Wu +5 more
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Electrophoretic and zymographic techniques for production monitoring of two lipase forms from Candida antarctica DSM 70725 [PDF]
Hemijska Industrija, 2012 Yeast Candida antarctica produces two lipase forms, which are widely used as catalysts in variety of organic reactions, many of which are applied on a large scale. In this work, production of two forms of lipase from C. antarctica DSM 70725 (CAL A and
Dimitrijević Aleksandra S. +3 more
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