Development and Validation of a Three-Step Screening Strategy for Extracellular Salt-Tolerant Nucleases From Marine Bacteria. [PDF]
Graphical Abstract and Lay Summary A three‐step screen for quick discovery of extracellular salt‐active/tolerant nucleases from halophiles–a Biotechnological Gold Mine. ABSTRACT This study reports the development of a 3‐step strategy that is both cost‐effective and quick to screen marine organisms and validate the presence of extracellular nucleases ...
Achar A, Sen S.
europepmc +2 more sources
Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B
Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 ...
Yaretzi J. Pedroza-Gómez +7 more
doaj +1 more source
Treatment with rumen microorganisms improves the methane fermentation of undegradable lignocellulosic biomass; however, the role of endoglucanase in lignocellulose digestion remains unclear.
Shuhei Takizawa +8 more
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Zymography for Picogram Detection of Lipase and Esterase Activities
Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity ...
Andre Mong Jie Ng +2 more
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A convenient zymogram stain for cellulases [PDF]
The release of dye from phosphoric acid-swollen cellulose azure by endocellulases and exocellulases is the basis of a technique for locating these enzymes on gels.
McHale, Anthony, Coughlan, Michael P.
openaire +2 more sources
Comparison of α-amylase isoforms from the midgut of Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae developed in the wild and on an artificial diet [PDF]
α-Amylase isoforms of Cerambyx cerdo larvae from the wild (ML and SL) and reared in the laboratory (ADL) were compared. Three amylase isoforms were presented in the SL and ML extracts while two isoforms were presented in the ADL according to zymogram ...
Dojnov Biljana +5 more
doaj +1 more source
ADAMTS-7 Increase Contributes to Worsened Neurological Outcome in Mice on High Fat Diet. [PDF]
High fat diet (HFD) increases a disintegrin and metalloproteinase‐7 (ADAMTS‐7) in the brain of mice with and without ischemia. Increased ADAMTS‐7 reduces tissue inhibitor of metalloproteinase‐1 (TIMP‐1) to increase matrix metalloproteinase 9 (MMP‐9) activity.
Xu Y, Li J, Zuo Z.
europepmc +2 more sources
Protein Involved in Initiation 1 Interaction With Starch Synthase 4 From Arabidopsis thaliana Induces Inhibition of Elongating Activity. [PDF]
ABSTRACT Starch is the major energy storage compound in plants. It accumulates in the form of insoluble, partly crystalline granules whose number and shape are specific to each plant species. These characteristics are defined very early in starch biosynthesis, at the initiation stage.
Bossu M +5 more
europepmc +2 more sources
The present study aimed to explore the optimal zymogram of combination of 6 carbohydrases (glucoamylase, pullulanase, maltase, thermostable α-amylase, medium temperature α-amylase, and cold-active α-amylase) supplemented in corn-soybean based diet of ...
Yang Liu +3 more
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TEKNIK ZYMOGRAM DAN APLIKASINYA DALAM RISET ENZIM
Teknik zymogram merupakan pengembangan dari teknik elektroforesis yang di dalam gel elektroforesis disisipkan substrat sehingga protein/enzim yang telah dipisahkan sekaligus dapat dipelajari kemampuannya dalam mendegradasi substrat tersebut.
Ekowati Chasanah
doaj +1 more source

