Results 121 to 130 of about 5,798 (171)

Structural and functional characterisation of isolated puff adder ( B. arietans ) serine proteases

open access: yes
Wilkinson MC   +5 more
europepmc   +1 more source

Differentiation of Bacterial Autolysins by Zymogram Analysis

Analytical Biochemistry, 2001
The use of zymograms in which the bacterial cell wall heteropolymer peptidoglycan is incorporated into the resolving gel of SDS-PAGE has led to the identification of various SDS stable peptidoglycan hydrolases (autolysins). To examine the specificity of autolysins with respect to O-acetylated peptidoglycan, a discontinuous SDS-PAGE system has been ...
Anthony Clarke
exaly   +3 more sources

Two-dimensional zymogram analysis of nucleases in Bacillus subtilis

Analytical Biochemistry, 1983
A two-dimensional zymogram procedure for the analysis of nucleases is described. Isoelectric focusing (IEF) and nonequilibrium pH gradient electrophoresis (NEPHGE) were compared as first dimensions in combination with sodium dodecyl sulfate (SDS) electrophoresis as the second dimension in analyzing nucleases in lysates of Bacillus subtilis.
S A, Coughlin, D M, Green
exaly   +3 more sources

A zymogram technique for the detection of carbohydrases

Analytical Biochemistry, 1973
Bert Pettersson   +2 more
exaly   +3 more sources

Zymogram of Pancreatic Lipases

Analytical Biochemistry, 2000
Abdelkarim Abousalham, Robert Verger
exaly   +3 more sources

Zymograms in Human Pathology

Nature, 1961
ENZYME histochemistry is sometimes a valuable aid in differentiating tumours in human pathology1 and in studying several other pathological processes2.
J, OORT, R G, WILLIGHAGEN
openaire   +2 more sources

Improved method for ribonuclease zymogram

Analytical Biochemistry, 1981
Abstract An improved method for ribonuclease zymogram in agarose overlays is described which enables significantly enhanced sensitivity and the avoidance of false-positive activity. Two critical aspects of the zymogram method—the fixation/staining technique and the RNA substrate concentration—were evaluated for the RNase activity in RNase A and in ...
J M, Thomas, M E, Hodes
openaire   +2 more sources

The Zymogram, in Clinical Medicine

American Journal of Clinical Pathology, 1965
The machinery of life is built of enzymes patterned in templates of nuclear material. Enzymes are complex polymers and they come in a variety of sizes, shapes, and shades. An enzyme which has a specific chemical catalytic function can be separated into a number of components in an electrical field, especially if the separation is carried out in starch ...
  +4 more sources

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