Results 131 to 140 of about 5,798 (171)

A positive zymogram method for ribonuclease

Analytical Biochemistry, 1979
Abstract We have developed a rapid, sensitive, and specific zymogram for detecting ribonuclease (RNase). The method makes use of an agarose gel containing the small substrate UpA [uridylyl (3′ → 5′)-adenosine]. UpA is hydrolyzed by RNase to adenosine, which is deaminated by adenosine deaminase. The inosine so formed is linked by a series of enzymatic
R C, Karn, M, Crisp, E A, Yount, M E, Hodes   +3 more
openaire   +2 more sources

A Versatile Negative-Staining Ribonuclease Zymogram

Analytical Biochemistry, 1994
A versatile negative-staining ribonuclease zymogram is described. The method has several advantages as it combines, by means of different staining procedures, high resolving power, sensitivity, and specificity with a rapid, reproducible, and simultaneous analysis of purity of ribonuclease samples on the same polyacrylamide gel.
J, Bravo, E, Fernández, M, Ribó, R, de Llorens, C M, Cuchillo   +4 more
openaire   +2 more sources

Endopolygalacturonase zymograms of Fusarium species

Transactions of the British Mycological Society, 1981
Endopolygalacturonase zymograms of 26 isolates belonging to six Fusarium species were detected by isoelectric focusing in polyacrylamide gels. Patterns of isolates of each species were identical except for isolates of F. moniliforme and F. solani that exhibited variations in intensity of bands. Comparison of various zymograms obtained showed that
SCALA, FELICE, CRISTINZIO, GENNARO, MARZIANO, FABRIZIO, NOVIELLO, CARMINE   +3 more
openaire   +2 more sources

Zymogram and life-history studies on trypanosomes of the subgenusMegatrypanum

Parasitology Research, 1990
Of 13 Swedish dairy cows examined, 12 (92.3%) were found to be infected with trypanosomes by cultivation of blood samples. Of the two species of tabanid fly caught close to the cattle, 33.3% of the Tabanus bromius and 8.6% of the Haematopota pluvialis were also found to be infected with trypanosomes on dissection.
M F, Dirie, S, Bornstein, K R, Wallbanks, J K, Stiles, D H, Molyneux   +4 more
openaire   +2 more sources

Calibrating Gelatin Zymograms with Human Gelatinase Standards

Analytical Biochemistry, 1996
We describe the use of gelatinase standards from human capillary whole blood for calibrating gelatin zymograms. Capillary blood was obtained by fingerstick puncture and prepared in nonreducing Laemmli SDS-PAGE sample buffer without heating. Gelatin zymography revealed that whole blood (0.25- 1 microliter) contained substantial fibroblast-derived (72 ...
G S, Makowski, M L, Ramsby
openaire   +2 more sources

Altered Esterase Zymograms associated with Infection with Leukaemia Virus

Nature, 1967
THE study of leukaemia viruses was until recently greatly hampered by the lack of cell cultures capable of propagating such viruses, but several cultures have now been found which support the replication of murine leukaemia virus. One of these cultures (JLS V6) originated from a mixture of BALB/c mouse spleen and thymus cells.
R L, Tyndall, R C, Allen
openaire   +2 more sources

Urinary pepsinogen zymogram in gastrectomized patients

The American Journal of Digestive Diseases, 1974
Urinary proteolytic activity and agar gel electrophoresis was done 3 months after complete gastrectomy in 7 patients, and the findings compared with those in 25 presumably normal subjects. It was found that 6 of the 7 gastrectomized patients showed proteolytic activity in the urine and that this enzymatic activity appeared as two or three bands on agar
J C, Mangla, Y, Kim
openaire   +2 more sources

PEROXIDASE ZYMOGRAMS OF 16 APPLE ROOTSTOCKS

Canadian Journal of Plant Science, 1986
Shoot bark and root extracts of 16 different apple (Malus domestica Borkh.) root-stocks were analyzed for multiple electrophoretic forms of peroxidase (EC 1.11.1.7) using polyacrylamide gel electrophoresis (PAGE). Thirteen bands were seen in the peroxidase zymograms of shoot bark extracts and eight in the peroxidase zymograms of root extracts.
DAVID L. WELLER, JOSEPH F. COSTANTE
openaire   +1 more source

Pectic zymograms and taxonomy and pathogenicity of the Ceratobasidiaceae

Transactions of the British Mycological Society, 1986
The pectic enzymes of 140 isolates of Rhizoctonia-1ike fungi from the Western Australian grainbelt were examined by electrophoresis and found to fall into 11 distinct zymogram groups (ZG). Isolates within a ZG had a similar cultural and morphological appearance and were either all multinucleate or all binucleate.
M.W. Sweetingham, R.H. Cruickshank, D.H. Wong   +2 more
openaire   +1 more source

Zymograms and histochemistry of non-specific esterase in the salivary glands

Histochemie, 1969
Zymogramic analysis of esterase in the mouse, rat and guinea-pig salivary glands was undertaken and demonstrated species and organ specificities of esterase with electrophoretic method. Salivary glands esterase was classified into A, B and C types based on the electrophoretic mobility.
Y, Yoshimura, M, Morishita, M, Mori, K, Kawakatsu   +3 more
openaire   +2 more sources