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Detection of Gelatinases by Substrate Zymography
Zymography is a method of electrophoretic separation of matrix metalloproteinases (MMPs) in a polyacrylamide gel-containing substrate (called zymogram gel) for the assay of various MMPs in different biological samples. In particular, gelatin-zymography allows to determine simultaneously both active and latent forms of MMP-2 (gelatinase A) and MMP-9 ...Emanuela, Falcinelli, Paolo, Gresele
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Measuring Matrix Metalloproteinases Activity by Gelatin Zymography
Gelatin zymography is a widely popular method due to its simplicity, low cost, and quick results, for studying gelatinases in various biological systems. Zymography can detect both the pro and active forms of matrix metalloproteinases MMP-2 (gelatinase A) and MMP-9 (gelatinase B).Kelli C, Micocci +2 more
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Assessment of Matrix Metalloproteinases by Gelatin Zymography
2016Matrix metalloproteinases are endopeptidases responsible for remodeling of the extracellular matrix and have been identified as critical contributors to breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression.
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Gelatin Zymography of Proteases in Chronic Wound Samples
Cellular processes such as tissue regeneration, inflammation, and migration require the proteolysis of the extracellular matrix and the proteolytic activation of signaling molecules. A widely used and accessible technique for studying these processes is gelatin zymography, particularly for investigating matrix metalloproteinases (MMPs), though it is ...Vojtěch, Pavlík, Aneta, Horčičková
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Enhanced Zymography of Proteases
Analytical Biochemistry, 1993C, Paech, T, Christianson, S, Bläsig
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Detection of Aspartic Proteinase Activities Using Gel Zymography
2017Gel zymography is a two-stage process where the proteins from the test sample are first separated by electrophoresis followed by the detection of the activity of hydrolytic enzymes. Many zymography procedures use sodium dodecyl sulfate (SDS) polyacrylamide gels copolymerized with an appropriate substrate.
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Zymography for the Detection of Bacterial Proteases
Proteases serve important functions for eukaryotes and bacteria, such as processing other proteins to activate them or degrading other proteins to create smaller peptides or eliminate malformed proteins. Proteases in pathogenic bacteria can also act as virulence factors by virtue of degradation of host proteins, activating other proteins that then ...openaire +2 more sources
Ultrathin Horizontal Sodium Dodecyl Sulfate Zymography
Analytical Biochemistry, 1995M P, Schönermark +3 more
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Soil zymography – A novel in situ method for mapping distribution of enzyme activity in soil
Soil Biology and Biochemistry, 2013Marie Spohn +2 more
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