Results 181 to 190 of about 26,894 (230)

Electrophoretic transfer protein zymography

Analytical Biochemistry, 2011
Zymography detects and characterizes proteolytic enzymes by electrophoresis of protease-containing samples into a nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel containing a copolymerized protein substrate. The usefulness of zymography for molecular weight determination and proteomic analysis is hampered by the ...
Daniel, Pan, Adam P, Hill, Anthony, Kashou, Karl A, Wilson, Anna, Tan-Wilson   +4 more
exaly   +3 more sources

In situ zymography: topographical considerations

Journal of Biochemical and Biophysical Methods, 2001
In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard,
Mungall, BA, Pollitt, CC
openaire   +3 more sources

Zymography, Casein Zymography, and Reverse Zymography: Activity Assays for Proteases and their Inhibitors

1999
Proteases and their inhibitors play an important role in the extracellular matrix (ECM) remodeling that occurs in a number of physiological and pathological processes. These include such diverse processes as trophoblast implantation, mammary gland involution, rheumatoid arthritis and tumor cell invasion (Liotta et al. 1991a; Liotta et al.
Gary W. Oliver, William G. Stetler-Stevenson, David E. Kleiner   +2 more
openaire   +1 more source

RAMA casein zymography: Time‐saving and highly sensitive casein zymography for MMP7 and trypsin

ELECTROPHORESIS, 2016
To detect metalloproteinase‐7 (MMP7), zymography is conducted using a casein substrate and conventional CBB stain. It has disadvantages because it is time consuming and has low sensitivity. Previously, a sensitive method to detect MMP7 up to 30 pg was reported, however it required special substrates and complicated handlings.
Hidetaro, Yasumitsu, Yasuhiro, Ozeki, Robert A, Kanaly   +2 more
openaire   +2 more sources

Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

2017
This chapter describes a method for the production and characterization of fungal acid proteases. Protease production is induced by growth on BSA media over a pH gradient and protein levels are monitored over time with the Bradford assay. Once protein is depleted, the media is purified and proteases are characterized by gelatin zymography using ...
Gavin, Kernaghan, Michael, Mayerhofer
openaire   +2 more sources

Analysis of Murein Hydrolases and Proteases Through Zymography

2021
Zymography has been used to analyze enzymatic activity and processing of enzymes for many years. We have used bacterial cells copolymerized into the acrylamide gel to analyze specific activity of murein hydrolases of interest. In addition, this method has been widely used to examine and distinguish protease activities using different substrates.
Nichole A, Seawell, Jeffrey L, Bose
openaire   +2 more sources

Loading-Controlled Zymography

Gelatin zymography is a widely used technique to study matrix metalloproteinases (MMP)-2 and -9, characterized by its simplicity, low cost, and high sensitivity. Zymography can detect picogram quantities of MMPs, which is 100-1000-fold more sensitive than immunoblot.
Aleksandra, Franczak, Paul, Jurasz
openaire   +2 more sources

Reverse Zymography: Overview and Pitfalls

2017
Reverse zymography is a technique by which protease inhibitor(s) in a sample could be electrophoretically separated in a substrate-impregnated acrylamide gel and their relative abundance could be semi-quantified. The gel after electrophoresis is incubated with a protease when the impregnated substrate and all other proteins of the sample are degraded ...
Kanika, Sharma, Debasish, Bhattacharyya
openaire   +2 more sources

Use of Zymography in Trypanosomiasis Studies

2017
Zymography assay is a semiquantitative technique, very sensitive, and commonly used to determine metalloproteinase levels in different types of biological samples, including tissues, cells, and extracts of protein. Samples containing metalloproteinases are loaded onto a polyacrylamide gel containing sodium dodecyl sulphate (SDS) and a specific ...
Jéssyka Fernanda Santiago, Monte, Cláudia Jassica Gonçalves, Moreno, Joana Patrícia Molato Figueiredo Lopes, Monteiro, Hugo Alexandre, de Oliveira Rocha, Aline Rimoldi, Ribeiro, Marcelo Sousa, Silva   +5 more
openaire   +2 more sources

Matrix metalloproteinase activity assays: Importance of zymography

Journal of Pharmacological and Toxicological Methods, 2010
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases capable of degrading extracellular matrix, including the basement membrane. MMPs are associated with various physiological processes such as morphogenesis, angiogenesis, and tissue repair.
K, Kupai, G, Szucs, S, Cseh, I, Hajdu, C, Csonka, T, Csont, P, Ferdinandy   +6 more
openaire   +2 more sources