Results 51 to 60 of about 11,350 (245)

Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis [PDF]

Journal of Bacteriology, 1992
The Zymomonas mobilis genes encoding alcohol dehydrogenase I (adhA), alcohol dehydrogenase II (adhB), and pyruvate decarboxylase (pdc) were overexpressed in Escherichia coli and Z. mobilis by using a broad-host-range vector containing the tac promoter and the lacIq repressor gene. Maximal IPTG (isopropyl-beta-D-thiogalactopyranoside) induction of these
V Worrell, Lonnie O. Ingram, N Arfman
openaire   +3 more sources

Covalently bound substrate at the regulatory site triggers allosteric enzyme activation [PDF]

, 2008
The mechanism by which the enzyme pyruvate decarboxylase from yeast is activated allosterically has been elucidated. A total of seven three-dimensional structures of the enzyme, of enzyme variants or of enzyme complexes form two yeast species (three of ...
Georg Wille, Manfred Weiss, Michael Spinka, Ralph Golbik, Steffen Kutter, Stephan Kö   +5 more
core   +1 more source

Enzymatic Synthesis of Enantiopure (R)‐Citronellal from Geraniol via a Short‐Chain Dehydrogenase and Ene Reductase

Advanced Synthesis &Catalysis, Volume 367, Issue 14, July 29, 2025.
A synthetic cascade with two consecutive biocatalytic steps consisting of a short‐chain alcohol dehydrogenase and an ene reductase in a biphasic system provides (R)‐citronellal with >90% conversion and >99% enantiomeric excess from inexpensive geraniol. The development of synthetic routes to produce enantiopure (R)‐citronellal as a key intermediate for
Claudia Ferrer‐Carbonell, Rocio Villa, Iris Viskaal, Diederik J. Opperman, Caroline E. Paul   +4 more
wiley   +1 more source

Effect of Starvation on the Viability and Cellular Constituents of Zymomonas anaerobia and Zymomonas mobilis [PDF]

Journal of General Microbiology, 1970
SUMMARY: Zymomonas anaerobia and Z. mobilis, grown on glucose + peptone + yeast-extract medium, degraded no endocellular carbohydrate, DNA and protein even in prolonged starvation. No significant qualitative changes in protein content during starvation were detected by disc gel electrophoresis of crude extracts. Both organisms had a high content of RNA
P. J. Large, E. A. Dawes
openaire   +3 more sources

Application of Zymomonas mobilis for bioethanol production from cellulose materials [PDF]

, 2012
Bakalářská práce pojednává o využití Zymomonas mobilis pro produkci bioethanolu z odpadního papíru. Byly použity tři druhy papíru od různých výrobců za účelem optimalizace produkce bioethanolu.
Čalová, Iveta
core  

Engineering pyruvate decarboxylase-mediated ethanol production in the thermophilic host Geobacillus thermoglucosidasius [PDF]

, 2013
This study reports the expression, purification, and kinetic characterization of a pyruvate decarboxylase (PDC) from Gluconobacter oxydans . Kinetic analyses showed the enzyme to have high affinity for pyruvate (120 μM at pH 5), high catalytic efficiency
A Correa, A Gupta, AA Kotze, AH Thompson, AJ Shaw, B Peters, C Bi, C Gustafsson, C Prust, D Gocke, D Meyer, D. A. Cowan, E Angov, E Angov, E Angov, G Lu, GL Rosano, HG Lawford, J Sambrook, JF Kane, JS Tolan, K Ohta, K. Eley, KC Raj, KC Raj, L. J. Van Zyl, LA Talarico, LA Talarico, LO Ingram, M Orencio-Trejo, M Pohl, M Pohl, M. P. Taylor, M. Tuffin, MP Taylor, MP Taylor, P Siegert, RE Cripps, RS Bongers, RS Gold, S Feldmann, S Kim, S Konig, S Konig, S Liu, S Liu, S Liu, SA Underwood, SA Underwood, SE Lowe, SJ Kaczowka, T Conway, TA Thanaraj, TE King, TF Clarke IV, UK Laemmli, Z Vuralhan   +56 more
core   +1 more source

Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes

ChemSusChem, Volume 18, Issue 8, April 14, 2025.
Recent advancements in the preparation of immobilized enzymes have improved both batch and continuous flow biocatalytic processes, essential for cost‐effective, sustainable production in the fine chemicals and pharmaceutical industries. This review examines advancements in carrier‐free methods, entrapment strategies, and support‐based approaches ...
Stefania Patti, Ilaria Magrini Alunno, Sara Pedroni, Sergio Riva, Erica Elisa Ferrandi, Daniela Monti   +5 more
wiley   +1 more source

EFECTO ANTAGÓNICO DE Zymomonas mobillis spp. FRENTE A Salmonella sp. y Proteus mirabi/is

Universitas Scientiarum, 2001
Se emplearon dos métodos microbiológicos para estudiar el antagonismo de 5 cepas de Zymomonas mobilis spp., frente a Proteus mirabilis y Salmonella sp. el método clásico de Ritter y el método ecométrico modificado.
Claudia M. Maldonado, Martín A. Bayona, Raúl A. Poutou   +2 more
doaj  

Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus [PDF]

, 2014
BACKGROUND: Bacterial pyruvate decarboxylases (PDC) are rare. Their role in ethanol production and in bacterially mediated ethanologenic processes has, however, ensured a continued and growing interest.
A Bjork, A Vagin, AA Kotze, AD Arencibia, AG Leslie, AK Chang, AT Baykal, B Chouaia, B Peters, CY Huang, D Dobritzsch, D Gocke, D Kern, D Meyer, D Meyer, D Rother, DKY Poon, Don A Cowan, E Potterton, E Zuckerkandl, EK James, G Lu, G Lu, G Schenk, G Vriend, GN Murshudov, GS Brandt, H Holzer, J Felsenstein, J Sambrook, JM Candy, K Lee, K Talley, K Tamura, KC Raj, KC Raj, LA Talarico, LA Talarico, Leonardo J van Zyl, LJ Van Zyl, LMS Lery, LMS Lery, LMS Lery, M Bertalan, M Couturier, M Mithran, M Pohl, M Tadege, MA Lie, Marla I Tuffin, MF dos Santosa, N Saitou, NS Nemeria, P Arjunan, P Emsley, P Evans, P Siegert, PM Kelley, PM Quigley, RA Frank, RA George, RS Bongers, RS Gokhale, RS Gold, S Bringer-Meyer, S Chakraborty, S Gomez-Manzo, S Kӧnig, S Lee, S Liu, S Liu, S Zhang, SE Lowe, SJ Kaczowka, TE King, U Menzel, UK Laemmli, VM Reis, VS Saravanan, WF Kellett, Wolf-Dieter Schubert, XY Pei, Z Dong, Z Vuralhan   +83 more
core   +2 more sources

From refuse to resource: exploring technological and economic dimensions of waste‐to‐energy

Biofuels, Bioproducts and Biorefining, Volume 19, Issue 2, Page 570-592, March/April 2025.
Abstract Waste valorization offers a sustainable approach to waste management and the generation of biofuels and bioenergy, mitigating the environmental impact of fossil fuel production and use. Carbon emissions and food‐source competition associated with first‐generation biofuels production can be mitigated.
Mohammed Al‐Hammadi, Mine Güngörmüşler   +1 more
wiley   +1 more source