Results 21 to 30 of about 1,987,921 (339)

Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction–Based SARS-CoV-2 Tests by Time Since Exposure

open access: yesAnnals of Internal Medicine, 2020
Background: Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on reverse transcriptase polymerase chain reaction (RT-PCR) are being used to “rule out” infection among high-risk persons, such as exposed inpatients and health ...
L. Kucirka   +4 more
semanticscholar   +1 more source

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

open access: yesBMC Bioinformatics, 2012
Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and ...
Jian Ye   +5 more
semanticscholar   +1 more source

Diagnostic Performance of CT and Reverse Transcriptase-Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis

open access: yesRadiology, 2020
Background Recent studies have suggested that chest computed tomography (CT) scans could be used as a primary screening or diagnostic tool for coronavirus disease 2019 (COVID-19) in epidemic areas.
Hyungjin Kim, Hyunsook Hong, S. Yoon
semanticscholar   +1 more source

False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

open access: yesKorean Journal of Radiology, 2020
The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide.
Dasheng Li   +6 more
semanticscholar   +1 more source

Contribution of neutrophil/lymphocyte ratio to the diagnostic efficiency of computed tomography and polymerase chain reaction in COVID-19 patients

open access: yesSAGE Open Medicine, 2021
Background: 6.5% of the country’s population was diagnosed with COVID-19 disease. Computed tomography scanning and polymerase chain reaction tests are considered reliable methods for the detection of COVID-19.
Serhat Örün, Mustafa Numan Erdem
doaj   +1 more source

On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

open access: yes, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction (Detection of Endophytic Fungi Using Polymerase Chain Reaction Technique)

open access: yesJurnal Fitopatologi Indonesia, 2013
Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control ...
Tuti Susanti Legiastuti, Tri Aminingsih
doaj   +1 more source

Detection of Brucella canis in blood, urine and seminal fluid of a naturally infected dog by PCR

open access: yesBrazilian Journal of Veterinary Medicine, 2018
A brucelose canina é uma doença bacteriana, infectocontagiosa que acomete cães de todo o mundo.O diagnóstico laboratorial é baseado em métodos diretos e indiretos e deve ser realizado quando há evidências de sinais clínicos e epidemiológicos.
Fabiana Sperb Volkweis   +5 more
doaj   +1 more source

Semi-quantitative digital analysis of polymerase chain reactionelectrophoresis gel: Potential applications in low-income veterinary laboratories [PDF]

open access: yesVeterinary World, 2016
Aim: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a ...
John F. Antiabong   +2 more
doaj   +1 more source

Computing by polymerase chain reaction [PDF]

open access: yesMathematical Biosciences, 2008
A mathematical notation is introduced to represent, at a symbolic level, different mechanisms of DNA recombination, and a 'PCR lemma' is proven by analytically describing the combinatorial properties of the polymerase chain reaction process. This approach led to the discovery of novel techniques, based on a form of PCR which we called cross pairing PCR
MANCA, Vincenzo, FRANCO, Giuditta
openaire   +3 more sources

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