Results 31 to 40 of about 49,242 (209)

Structural Control of Dual Emission in Coumarin Fluorophores for Visualizing Protein Droplet Maturation

Angewandte Chemie, EarlyView.
A coumarin‐based dual‐emission fluorophore was designed to visualize the maturation of protein liquid droplets. Upon covalent conjugation to droplet‐forming proteins, ratiometric fluorescence imaging allowed real time monitoring of droplet maturation. This probe is applicable to the analysis of droplet formation and maturation mechanisms, as well as to
Tomoya Yamamoto, Yusei Otsuka, Asuka Mori, Yuichiro Hori, Yuma Yamamoto, Motonari Uesugi, Kazuya Kikuchi   +6 more
wiley   +2 more sources

Changes in Metabolites from Bovine Milk with β-Casein Variants Revealed by Metabolomics

Animals, 2020
β-casein is a primary protein in milk, and its variants have been associated with changes in the protein content of bovine milk. However, there has been little research focused on the effects of β-casein variants on milk metabolites. In the present study,
Zhongwang Lv, Hui Liu, Yongxin Yang, Dengpan Bu, Changjiang Zang, Kailun Yang, Xiong Yu, Jiaqi Wang   +7 more
doaj   +1 more source

Additional file 1: of Clinical evaluation of glutathione concentrations after consumption of milk containing different subtypes of β-casein: results from a randomized, cross-over clinical trial

, 2016
Detailed Method to isolate and measure BCM-7. (DOC 189 kb)
Deth, Richard, Clarke, Andrew, Ni, Jiayi, Trivedi, Malav   +3 more
openaire   +1 more source

Knock-in of Enhanced Green Fluorescent Protein or/and Human Gene into Gene Locus in the Porcine Fibroblasts to Produce Therapeutic Protein [PDF]

Asian-Australasian Journal of Animal Sciences, 2014
Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the ...
Sang Mi Lee, Ji Woo Kim, Young-Hee Jeong, Se Eun Kim, Yeong Ji Kim, Seung Ju Moon, Ji-Hye Lee, Keun-Jung Kim, Min-Kyu Kim, Man-Jong Kang   +9 more
doaj   +1 more source

Investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk

AMB Express, 2017
A novel endogenous peptide cleaved from 197–213 AA of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk.
Yanrong Fu, Chenbo Ji, Xiaohui Chen, Xianwei Cui, Xing Wang, Jie Feng, Yun Li, Rui Qin, Xirong Guo   +8 more
doaj   +1 more source

Use of Mass Spectrometry to Profile Peptides in Whey Protein Isolate Medium Fermented by Lactobacillus helveticus LH-2 and Lactobacillus acidophilus La-5

Frontiers in Nutrition, 2019
Peptides in the 3-kDa ultrafiltrate of fermented whey protein isolate (WPI) medium could be responsible for the antivirulence activity of Lactobacillus helveticus LH-2 and Lactobacillus acidophilus La-5 against Salmonella Typhimurium.
Eman Ali, Eman Ali, Søren D. Nielsen, Salah Abd-El Aal, Ahlam El-Leboudy, Ebeed Saleh, Gisèle LaPointe   +6 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Identification of Bovine Casein Phosphorylation Using Titanium Dioxide Enrichment in Combination with Nano Electrospray Ionization Tandem Mass Spectrometry

Journal of Integrative Agriculture, 2012
Protein phosphorylation is an important post-translational modification that regulates milk protein structure and function. The objective of this study was to analyze the presence of phosphorylated casein. Bovine milk proteins were first separated by SDS
Shan-shan LI, Jia-qi WANG, Hong-yang WEI, Yong-xin YANG, Deng-pan BU, Le-ying ZHANG, Ling-yun ZHOU   +6 more
doaj   +1 more source

Spatiotemporal Control of Formation of Dynamic Protein Fiber Assemblies via Photophysical Effects of a Focused Laser Beam

Advanced Science, EarlyView.
Tightly focused laser irradiation can accumulate tubulin proteins at/around the laser focus, which leads to the formation of highly ordered microtubule assemblies. The assemblies can exhibit various dynamic behaviors such as radial motion, bundling, and flagella‐like rotation with motor protein and chemical energy, highlighting as a unique tool for ...
Hiroshi Y. Yoshikawa, Ren Shirata, Takuya Takeshige, Riki Yoshida, Fumika Kiryu, Kei Takano, Shuma Matsumoto, Reiji Kawanami, Natsumi Sawada, Chi‐Shiun Wu, Yang‐Hsin Shih, Hiromasa Niinomi, Takahisa Matsuzaki, Seiichiro Nakabayashi, Tomoaki Matsuura, Teruki Sugiyama, Ryuzo Kawamura   +16 more
wiley   +1 more source

CK2α Deficiency Drives Myocardial Fibrosis via Desmin‐Induced Mitochondrial Dysfunction

Advanced Science, EarlyView.
CK2α preserves mitochondrial homeostasis by phosphorylating Desmin to recruit Cryab, ensuring proper filament assembly. CK2α deficiency disrupts this interaction, causing mitochondrial dysfunction, metabolic shifts, bioenergetic failure, and oxidative stress—ultimately establishing a pro‐fibrotic environment that drives cardiac fibrosis.
Canjie Ma, Jiali Jia, Juncong Lan, Jing Wang, Dan Rao, Lanlan Rao, Weibin Zhang, Dongpeng Wu, Jie Zhang, Gang Wang, Baohua Liu, Ying Ao, Zimei Wang   +12 more
wiley   +1 more source