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Escherichia coli β-Lactamases: What Really Matters
Escherichia coli strains belonging to diverse pathotypes have increasingly been recognized as a major public health concern. The β-lactam antibiotics have been used successfully to treat infections caused by pathogenic E. coli.
Priyanka eBajaj +2 more
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Phenotypic detection of Metallo- and AmpC β-lactamases producing strains of P. aeruginosa in the state of Himachal Pradesh (India) [PDF]
Background: A prospective study was undertaken to detect metallo-β-lactamases (MBLs) and AmpC β-lactamases in Pseudomonas aeruginosa isolates recovered from patients at Indira Gandhi Medical College, Shimla (H.P).
Naveen Minhas, Prakash Chand Sharma
doaj
Additional file 2: Table S2. Primer sequences used for A. baumannii in present study.
Raible, Kevin +6 more
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Penetration of β-lactamase inhibitors into the periplasm of Gram-negative bacteria [PDF]
The effectiveness of a beta-lactamase inhibitor/beta-lactam combination against Gram-negative pathogens depends on many interplaying factors, one of which is the penetration of the inhibitor across the outer membrane. In this work we have measured the relative penetrations of clavulanic acid, sulbactam, tazobactam and BRL 42715 into two strains of ...
Farmer TH, Degnan BA, Payne DJ
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This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013.
Diana Yessenia Calva Delgado +4 more
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Cefotaxime-hydrolysing activity of the β-lactamase ofKlebsiella oxytocaD488 could be related to a threonine residue at position 140 [PDF]
The chromosomally encoded β-lactamase of Klebsiella oxytoca D483 strain, active against all third-generation cephalosporins but ceftazidime, was purified to homogeneity. The pure protein was digested by trypsin, Staphylococcus aureus V8 protease or proteinase Asp-N.
Alain Reynaud +3 more
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Detection of inducible β-lactamase by an agar dilution technique [PDF]
An agar dilution technique for the detection of inducible β-lactamase-mediated resistance to the newer cephalosporins is described. Cefoxitin (16 μg/ml) was incorporated in agar plates together with cefamandole (8 μg/ml) or cefotaxime (8 μg/ml). The susceptibility of 35 strains of Enterobacter cloacae to these combinations was compared with their ...
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Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable β-lactamases (TEM, SHV, CARB) [PDF]
Intragenic DNA probes were synthesized by polymerase chain reaction using fragments of the genes of three major types of β-lactamases (TEM, SHV, CARB) as templates. The TEM probe hybridized with the genes encoding TEM-1, TEM-2 and six extended-spectrum related enzymes (TEM-3 to TEM-7, TEM-20) in colony hybridizations and Southern-blot analysis. The SHV
G. Arlet, A. Philippon
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Background: Escherichia coli belong to the family of Enterobacteriaceae that are responsible as one of the leading cause of nosocomial infections. The emergence of Carbapenem-resistant Enterobacteriaceae presents therapeutic challenges for clinicians on choosing the correct type of antibiotics to prescribe.
Muhammad Ardi Afriansyah +2 more
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