Results 61 to 70 of about 671,192 (298)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

The concept of the Sun as ἡγεμονικόν in the Stoa and in Manilius’Astronomica

Revista Archai, 2017
Hegemonikon in Stoic vocabulary is the technical term for the chief part or command-centre’of the soul. As we know, the Stoics considered the cosmos a living organism, and they theorised both about the human soul’s Hegemonikon and about its counterpart in the World-soul.
openaire   +3 more sources

On (μ1, μ2, μ3)-Weakly Generalized Closed Sets

European Journal of Pure and Applied Mathematics, 2020
This paper defines a new generalization of closed sets in a tri-generalized topological space called (μ1, μ2, μ3)-weakly generalized closed set (or briefly (μ1,μ2, μ3)-wg closed set) which is defined as follows: A subset A of X is (μ1, μ2, μ3)-weakly generalized closed set if clμ1(intμ2(A)) ⊆U whenever A ⊆ U and U is μ3-open in X.
Breix Michael Agua, Rolando N. Paluga
openaire   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Διάχυτη Ενδοαγγειακή Πήξη: Παθοφυσιολογία, Διάγνωση, Αντιμετώπιση και η σχέση της με την κύηση και τον καρκίνο [PDF]

Epistīmonika Chronika, 2018
Η διάχυτη ενδαγγειακή πήξη (ΔΕΠ) χαρακτηρίζεται από συστηματική ενεργοποίηση του μηχανισμού της πήξης με αποτέλεσμα την παραγωγή ινώδους και την δημιουργία θρόμβων σε διάφορα όργανα, με τελική κατάληξη την πολυοργανική ανεπάρκεια.
Α. Περδίου, Δ. Συμεωνίδης, Κ. Σταθερού, Α. Κουσουλάκου   +3 more
doaj  

Electrochemical characteristics of the La0.9Sr0.1Sс0.4Mn0.6O3 – δ cathode contacting the La0.9Sr0.1SсO3 – δ proton-conducting electrolyte [PDF]

Электрохимическая энергетика
In this work, the material La0.9Sr0.1Sс0.4Mn0.6O3 – δ was synthesized using the citrate-nitrate method. The electrochemical characteristics of the material were studied using impedance spectroscopy on symmetric cells and compared with the ...
Bervitskaya, Olga S., Ichetovkina, Victoria A., Bobro, Mark S., Stroeva, Anna Y., Kuzmin, Anton Valerievich   +4 more
doaj   +1 more source

δD, δ¹⁸O and δ¹³C concentrations in water samples of the East Siberian Sea from cruise AMK69 in 2017

, 2020
Oxygen, hydrogen, and carbon isotope data with the [DIC] concentrations were analyzed in water samples collected in the East Siberian Sea along the Kolyma and Indigirka sub-meridian profile in route 69 of R/V "Akademik Mstislav Keldysh" in 2017. Sampling
Dubinina, Elena O, Kossova, Sofia, Chizhova, Julia Nikolaevna, Miroshnikov, Alexsey Y, Avdeenko, Anna S   +4 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source