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陕西地区静脉血栓栓塞症患者MTHFR和MTRR基因多态性分析以及对临床诊疗的指导意义

Zhongguo shiyan zhenduanxue, 2022
目的 探讨陕西地区静脉血栓栓塞症患者MTHFR和MTRR基因多态性分布情况。方法 选取西安交通大学第一附属医院静脉血栓栓塞症治疗患者439例，检测MTHFR和MTRR的基因型，统计基因型及等位基因分布特征，并对比国内外其他地区基因型及等位基因分布差异。结果 MTHFR和MTRR基因型分布CT、TT、CC;AA、AG、GG，分别占患者总数的48.06%、30.07%、21.87%;67.43%、28.93%、3.64%。MTHFR CC及TT等位基因频率分别为45.90%和54.10%;MTRR ...
张李婷, 马文兵, 关栋, 邓祥
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多重耐药鲍曼不动杆菌相关耐药基因的研究

Zhongguo shiyan zhenduanxue, 2021
目的 研究院内MDRAB相关耐药基因及分子流行病学特征,为抗菌药物合理使用及医院感染控制提供依据。方法 对2019年2月至2019年10月间院内分离的15株MDRAB用多位点序列分型（MLST）鉴定菌株之间的亲缘关系,PCR法检测β-内酰胺类耐药基因、质粒介导的喹诺酮类耐药基因、氨基糖苷类耐药基因及消毒剂qacE△1基因。结果 MLST分析发现15株MDRAB有ST195（46.7%）、ST369（20%）、ST208（13.3%）、ST368（13.3%）和ST540（6.7%）5个序列类型 ...
姜梅杰, 朱刚, 王薇, 陈霞, 赵书平   +4 more
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Research progress on heat resistance of Rhododendron germplasm resources(杜鹃花种质资源耐热性研究进展)

浙江大学学报. 农业与生命科学版
China has the richest germplasm resources of Rhododendron, but most species thrive in high-altitude cool areas. Currently, only a limited number of these species are utilized in landscaping, and their summer performance is generally poor.
曹雨晴(CAO Yuqing), 刘梦璇(LIU Mengxuan), 瞿方茜(QU Fangqian), 王琪(WANG Qi), 周泓(ZHOU Hong), 夏宜平(XIA Yiping), 王秀云(WANG Xiuyun)   +6 more
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Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定)

浙江大学学报. 农业与生命科学版
In this study, evolutionary and expression analyses of the P450 gene family in Rosa chinensis were conducted using bioinformatics analysis methods, and the mechanism by which these genes are involved in the synthesis of rose fragrance was explored.
王君妍(WANG Junyan), 王峰(WANG Feng), 龚瑶(GONG Yao), 方慧仪(FANG Huiyi), 张亮生(ZHANG Liangsheng)   +4 more
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Physiological measurement and screening of key genes of the rhizome cold tolerance in the herbaceous peony cultivated over winter in Harbin(哈尔滨越冬栽培的芍药根茎耐寒生理测定和关键基因筛选)

浙江大学学报. 农业与生命科学版
Paenonia lactiflora Pall. is a renowned traditional Chinese flower, and it is of great significance to study its cold tolerance mechanism for breeding new varieties and expanding its popularization.
王琪瑶(WANG Qiyao), 李丹青(LI Danqing), 郭俊宏(GUO Junhong), 陈晓璇(CHEN Xiaoxuan), 陈炀(CHEN Yang), 夏宜平(XIA Yiping), 张佳平(ZHANG Jiaping), 刘志洋(LIU Zhiyang)   +7 more
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Research progress on the underground-bud dormancy mechanisms of ornamental flowers(观赏花卉的地下芽休眠机制研究进展)

浙江大学学报. 农业与生命科学版
The underground-bud plants, namely, the geophytes, constitute an important category in bud classification of plants. These plants include a significant number of ornamental flowers.
郭俊宏(GUO Junhong), 李丹青(LI Danqing), 王琪瑶(WANG Qiyao), 赵伯国(ZHAO Boguo), 任梓铭(REN Ziming), 陈晓璇(CHEN Xiaoxuan), 吴双喆(WU Shuangzhe), 钟抒昀(ZHONG Shuyun), 刘志洋(LIU Zhiyang), 张佳平(ZHANG Jiaping)   +9 more
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Establishment of a homologous gene silencing system and gene function verification of bulb formation in Lycoris Herb.(石蒜同源基因沉默体系构建及鳞茎发生基因功能验证)

浙江大学学报. 农业与生命科学版
In this study, we explored the construction of a homologous gene silencing system for Lycoris insularis, which is a rich wild resource in Zhejiang Province with bulbs as explants, and through this system, the biological functions of Lycoris insularis ...
肖潇(XIAO Xiao), 夏宜平(XIA Yiping), 崔柳(CUI Liu), 张佳平(ZHANG Jiaping), 洪震(HONG Zhen), 任梓铭(REN Ziming)   +5 more
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AS-PCR法检测CLU基因SNP位点方法的建立

Zhongguo shiyan zhenduanxue, 2019
目的基于等位基因特异性PCR(allele-specific PCR,AS-PCR)原理,建立一种QPCR检测CLU基因与阿尔兹海默病(AD)相关性SNP位点基因型的筛选方法。方法根据CLU基因上的rs11136000,rs2279590和rs9331888 3个SNP位点设计特异性的野生型、突变型引物,使用实时荧光定量PCR(QPCR)对AD患者外周血样本进行SNP基因分型,以DNA基因测序法结果作为参考标准。结果 10例样本的QPCR定量分型结果显示 ...
李逸豪, 石冰洁, 冯娅茹, 李晓瑞, 朱晶晶, 田金洲, 时晶, 倪敬年, 王建勋   +8 more
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邯郸市乙型肝炎基因分型及其临床意义研究

Zhongguo shiyan zhenduanxue, 2010
目的研究不同类型乙型肝炎病毒（HBV）感染者中HBV基因型分布情况及其临床意义。方法应用基因型特异性引物聚合酶链反应法（PCR）,对180份HBV感染者血清进行基因型分型。结果180份标本中,162份（90%）可检出基因型,18份（10%）未检出基因型。162份HBV基因型分布为:B基因型1例（0.6%）,C基因型159例（98.1%）,B+C混合基因型2例（1.2%）,未检出A、D、E、F基因型。结论邯郸地区HBV感染者中,基因型分布较单一,C基因型占绝对优势,同时存在少量B基因型及B+C混合基因型 ...
邓健, 马艳霞, 郭娜娜, 李瑞芳, 李杰, 史宇辉, 郭瑞玲   +6 more
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ID4基因的研究进展

Zhongguo shiyan zhenduanxue, 2011
癌基因的活化（如ras基因、bcr-abl基因）或/和抑癌基因的失活（如P53基因、Rb基因）与肿瘤的发生密切相关。ID4基因是1994年Riechmann等首次从小鼠中分离鉴定的,发现该基因在胚胎发育的9.5d-13.5d是上调的,且在成人的脑、睾丸和肾脏器官高表达[1]。
冯燕, 李薇, 党艳辉, 卢学春
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