Results 31 to 40 of about 953,736 (207)
, 2000 采用聚丙烯酰胺梯度凝胶电泳及特异性组织化学染色技术分析了鲤、鲫及其基因组叠加的不同组合个体的红细胞ＳＯＤ． ＥＳＴ和血红蛋白的电泳图谱。结果表明不同基因组组合及不同倍性的个体间存在由基因组差异导致的生化多态性，为不同倍性的鱼类的同工酶（ＳＯＤ）表达存在基因剂量效应提供了依据，证实了不同组合的ＳＯＤ亚基间有协同表达、累积作用。研究表明每个基因有其独特的调控机制，因而在杂种三倍体内三分之一的基因组亦能够表达产物。应用本研究的遗传学数据有益于构建基因组特异性品系 ...
吴玉萍,叶玉珍,吴清江
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International Journal of Applied Linguistics, EarlyView.ABSTRACT With the increasing number of Chinese international students in Australian universities, intercultural competence has become a critical factor influencing their interactions with Australian tutors in university classroom settings. Existing research often examines students’ intercultural competence from a single‐method or single‐perspective ...
Jiangyu Han, Linda Tsung
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的评价基因芯片技术在HBV基因型及HBVP基因YMDD变异检测中的应用价值,并对不同基因型病例的临床情况进行分析。方法抽提HBV DNA,用PCR方法扩增S基因及P基因部分区域,扩增产物与基因芯片上的寡核苷酸探针进行杂交,杂交结果通过芯片影像读取仪扫描到计算机上,经软件分析可得到HBV基因型、HBVYMDD野生型及YVDD、YIDD变异型结果;部分标本通过HBV preS/S基因序列测序证实。结果38份高病毒滴度标本（HBV DNA定量>1.0×105拷贝/毫升）中22例为B基因型（58 ...
陈伟烈 +5 more
doaj
, 2011 隨著生物技術的發展，使得分子生物學家和育種家得以藉由DNA 重組技術針對特定的植物遺傳性狀直接進行改造，此即所謂的基因轉殖技術，它提供了嶄新的植物品種改良方法。此一新技術的應用目前在國際間正引發不同的考量與反應，一方面由於基因改造作物有助於提高並擴大原作物的栽培價值，因而廣受各國重視而競相研發；另一方面則有人對其推廣懷著隱憂，認為可能對地球生態及食品安全帶來負面影響。因此，為顧及應用上的安全，轉基因作物在推廣前須有妥善的評估與研究，以防止可能的副作用。為此，近十餘年來政府不僅努力於相關法規制度的建立 ...
賴吉雄 +4 more
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International Journal of Applied Linguistics, EarlyView.ABSTRACT Many existing rubrics for L2 argumentative writing prioritize form‐based linguistic accuracy and complexity while insufficiently addressing substantive argumentative rigor and effectiveness. This limitation is further complicated by cross‐cultural variation in rhetorical conventions and educational practices, which challenge the direct ...
Zihan Xu, Peiyun Liu
wiley +1 more source
四川大学学报. 自然科学版, 2011 BnGID1基因是本实验室克隆到的AtGID1基因在甘蓝型油菜(Brassica nupus L.)中的同源基因.为了验证该基因在甘蓝型油菜中的功能,我们利用pFGC5941双元载体构建了BnGID1基因的干扰载体并将其转化入甘蓝型油菜野生型"3529"中,得到了BnGID1基因表达被部分抑制的转基因植株.性状观察证明,在BnGID1基因表达被部分抑制后,植株表现出明显的接化特征,高度显著降低,叶片产生皱缩.与水稻,拟南芥中的GID1基因缺失突变体的性状比较证明BnGID1基因具有与OsGID1 ...
李华鹏 +3 more
doaj
, 2012 单细胞原核生物是原始的细胞生命形式,确定细菌必需基因和最小基因组对理解生命的本质、细胞生命的起源和进化有非常重要的意义。文中简要介绍近年来有关细菌的必需基因、最小基因组和合成细胞的研究方法、理论和进展 ...
邱东茹
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Exploring Peer L2 Grit in Relation to Grit and Motivation
International Journal of Applied Linguistics, EarlyView.ABSTRACT Grit has attracted considerable attention because of its positive association with second language (L2) learning; however, its socio‐contextual dimensions remain underexplored. Specifically, little is known about how learners’ perceptions of their peers’ perseverance and sustained interest in L2 learning, conceptualized in this study as peer ...
Mitsuko Tanaka +2 more
wiley +1 more source
血管紧张素转换酶、内皮型一氧化氮合酶和载脂蛋白E基因多态性与冠心病的相关性
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 [目的]研究血管紧张素转换酶（ACE）基因插入/缺失多态性、内皮型一氧化氮合酶（eNOS）基因G894T多态性和载脂蛋白E（ApoE）基因多态性与冠状动脉粥样硬化性心脏病（冠心病）的关系.[方法]应用基因芯片技术分析133例冠心病患者和122例对照者ACE、eNOS和ApoE基因多态性,对比两组基因型及等位基因频率.[结果]冠心病组ACE DD基因型频率比对照组显著升高,28.6% vs 13.1%,P＜0.01,ACE基因多态性与冠心病危险性相关.两组eNOS和ApoE基因型频率差异无统计学意义（P ...
梁茜 +4 more
doaj
Exploring English Language Learners’ Vocabulary Knowledge and Usage When Speaking
International Journal of Applied Linguistics, EarlyView.ABSTRACT Vocabulary forms a fundamental component of speaking ability, as it enables speakers to convey meaning accurately, fluently, and effectively. Although the relationship between vocabulary and speaking has been investigated to some extent, gaps remain in understanding the link between learners’ vocabulary knowledge and their speaking proficiency,
Junyan Guo, Martin East
wiley +1 more source