Results 31 to 40 of about 763,879 (236)
单细胞原核生物是原始的细胞生命形式,确定细菌必需基因和最小基因组对理解生命的本质、细胞生命的起源和进化有非常重要的意义。文中简要介绍近年来有关细菌的必需基因、最小基因组和合成细胞的研究方法、理论和进展 ...
邱东茹
core
Evaluating synthetic substitutes to reduce illegal harvesting and support species recovery
Abstract Providing synthetic substitutes is a widely promoted strategy to shift consumer demand away from wildlife products derived from threatened species. Yet, there is little evidence on whether product substitution prevents illegal or unsustainable harvesting and contributes to the recovery of threatened populations.
Aditya Shekhar Malgaonkar +17 more
wiley +1 more source
目的评价基因芯片技术在HBV基因型及HBVP基因YMDD变异检测中的应用价值,并对不同基因型病例的临床情况进行分析。方法抽提HBV DNA,用PCR方法扩增S基因及P基因部分区域,扩增产物与基因芯片上的寡核苷酸探针进行杂交,杂交结果通过芯片影像读取仪扫描到计算机上,经软件分析可得到HBV基因型、HBVYMDD野生型及YVDD、YIDD变异型结果;部分标本通过HBV preS/S基因序列测序证实。结果38份高病毒滴度标本(HBV DNA定量>1.0×105拷贝/毫升)中22例为B基因型(58 ...
陈伟烈 +5 more
doaj
Abstract Knowledge of species distributions is essential for informing policies on nature conservation and restoration. However, updating them on a regular basis and doing so in a harmonized manner at the international level is difficult. The European Bird Census Council integrated national monitoring data covering 5 years to update farmland bird ...
Sergi Herrando +54 more
wiley +1 more source
BnGID1基因是本实验室克隆到的AtGID1基因在甘蓝型油菜(Brassica nupus L.)中的同源基因.为了验证该基因在甘蓝型油菜中的功能,我们利用pFGC5941双元载体构建了BnGID1基因的干扰载体并将其转化入甘蓝型油菜野生型"3529"中,得到了BnGID1基因表达被部分抑制的转基因植株.性状观察证明,在BnGID1基因表达被部分抑制后,植株表现出明显的接化特征,高度显著降低,叶片产生皱缩.与水稻,拟南芥中的GID1基因缺失突变体的性状比较证明BnGID1基因具有与OsGID1 ...
李华鹏 +3 more
doaj
隨著生物技術的發展,使得分子生物學家和育種家得以藉由DNA 重組技術針對特定的植物遺傳性狀直接進行改造,此即所謂的基因轉殖技術,它提供了嶄新的植物品種改良方法。此一新技術的應用目前在國際間正引發不同的考量與反應,一方面由於基因改造作物有助於提高並擴大原作物的栽培價值,因而廣受各國重視而競相研發;另一方面則有人對其推廣懷著隱憂,認為可能對地球生態及食品安全帶來負面影響。因此,為顧及應用上的安全,轉基因作物在推廣前須有妥善的評估與研究,以防止可能的副作用。為此,近十餘年來政府不僅努力於相關法規制度的建立 ...
賴吉雄 +4 more
core
Informal supply chains of wild meat from rural Amazonia and food security in an urban center
Abstract Iquitos, the most populated city in the Peruvian Amazon, is a hub in the regional supply chain of wild meat and supplies urban consumers. Studies on wild meat consumption have focused primarily on markets, limiting the scope of species considered to those that are economically valuable and potential inferences from those data.
Fiorella Briceño Huerta +3 more
wiley +1 more source
血管紧张素转换酶、内皮型一氧化氮合酶和载脂蛋白E基因多态性与冠心病的相关性
[目的]研究血管紧张素转换酶(ACE)基因插入/缺失多态性、内皮型一氧化氮合酶(eNOS)基因G894T多态性和载脂蛋白E(ApoE)基因多态性与冠状动脉粥样硬化性心脏病(冠心病)的关系.[方法]应用基因芯片技术分析133例冠心病患者和122例对照者ACE、eNOS和ApoE基因多态性,对比两组基因型及等位基因频率.[结果]冠心病组ACE DD基因型频率比对照组显著升高,28.6% vs 13.1%,P<0.01,ACE基因多态性与冠心病危险性相关.两组eNOS和ApoE基因型频率差异无统计学意义(P ...
梁茜 +4 more
doaj
Abstract Procedural, distributional, recognitional, and epistemic justice aspects of conservation interventions are well documented in contexts where pastoralism is a key livelihood and way of life. Geospatial analyses and representations of wildlife conservation and restoration that are increasingly applied in pastoralist rangeland socioecological ...
Ryan R. Unks
wiley +1 more source

