Results 41 to 50 of about 71,543 (200)

p38MAPK induces apoptosis of glioma cell [PDF]

open access: yes, 2001
目的 研究 p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响 .方法 利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系 C6中 ,用免疫细胞化学染色检测其在细胞转染前后的表达情况 ,用 HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响 .结果 转染 p CMV5 - p38MAPK质粒组 p38MAPK蛋白表达阳性 ,细胞形态发生变化 ,贴壁性降低 ,出现大量凋亡细胞 .结论 转染 ...
叶菁(第四军医大学西京医院病理科)   +6 more
core  

Advancing conservation breeding programs for marine invertebrates

open access: yesConservation Biology, EarlyView.
Abstract In the face of ecosystem change and biodiversity loss caused by climate change and other stressors, conservation breeding, or captive breeding, with the aim of reintroduction for wild population recovery, is an emerging tool for preventing species’ extinction and rehabilitating ecosystems.
Elora H. López‐Nandam   +3 more
wiley   +1 more source

雌激素受体α基因多态性与女孩性早熟关系的研究

open access: yesZhongguo shiyan zhenduanxue, 2010
目的探讨雌激素受体-α(ERα)基因多态性与江西地区女孩性早熟的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术,检测90例江西地区性早熟女孩和70例江西地区健康体检女孩ERα基因内含子1 Pvu Ⅱ T/C和XbaI A/G酶切位点基因多态性,并观察基因型对血清E2的影响。结果病例组ERα基因XbaⅠ基因型和等位基因频率分布与对照组相比差异有显著性 ...
柯江维   +6 more
doaj  

Research on expression of HIV-1 env in baculovirus [PDF]

open access: yes, 2000
昆虫细胞杆状病毒表达系统(BEVS)是真核表达系统的一种,以一种无需额外辅助的重组昆虫杆状病毒作为表达载体,目前已被应用来表达不同来源的基因,如细菌、病毒、植物和哺乳动物的基因。它具有表达产量高,蛋白经过完善的后处理过程,如内含子剪切、糖基化等特点。其原理是通过外源基因置换病毒的核多角体蛋白基因,使外源基因在核多角体蛋白启动子下高效表达。本论文中采用BEVS表达HIV-1包膜蛋白,目的在于弥补原核表达系统不能表达大片段的、高度糖基化的重组蛋白;并探索其应用在检测试剂中的前景。Baculovirus ...
郭庆
core  

Spatial identification of areas suitable for other effective area‐based conservation measures in the European Union

open access: yesConservation Biology, EarlyView.
Abstract Although significant biodiversity has been safeguarded by protected areas (PAs), biodiversity trends continue downward. Within the frameworks of the EU Biodiversity Strategy for 2030 and the new EU Restoration Regulation (2024), conserving critical biodiversity areas is essential.
George Kefalas   +14 more
wiley   +1 more source

基因芯片技术在乙肝病毒基因型及YMDD变异检测中的应用

open access: yesZhongguo shiyan zhenduanxue, 2007
目的评价基因芯片技术在HBV基因型及HBVP基因YMDD变异检测中的应用价值,并对不同基因型病例的临床情况进行分析。方法抽提HBV DNA,用PCR方法扩增S基因及P基因部分区域,扩增产物与基因芯片上的寡核苷酸探针进行杂交,杂交结果通过芯片影像读取仪扫描到计算机上,经软件分析可得到HBV基因型、HBVYMDD野生型及YVDD、YIDD变异型结果;部分标本通过HBV preS/S基因序列测序证实。结果38份高病毒滴度标本(HBV DNA定量>1.0×105拷贝/毫升)中22例为B基因型(58 ...
陈伟烈   +5 more
doaj  

Functional Study of SIDP364 and SIDP361 Genes of DUF1644 Family in Salt Stress Response [PDF]

open access: yes, 2012
水稻是重要的粮食作物,也是单子叶植物研究的模式植物。高盐、冷害和干旱等各种非生物逆境对作物的生长发育、产量和品质都有影响,而土壤盐渍化是农作物最主要的非生物胁迫之一,因此对水稻耐盐性进行研究意义重大。水稻的耐盐性是由多基因控制的复杂性状,涉及到胁迫应答、离子平衡和物质运输等诸多方面。通过基因芯片和生物信息学分析,从水稻中分离了两个基因SIDP364(StressinducedDUF1644Protein364)和SIDP361(StressinducedDUF1644Protein364 ...
李敏
core  

Evaluating synthetic substitutes to reduce illegal harvesting and support species recovery

open access: yesConservation Biology, EarlyView.
Abstract Providing synthetic substitutes is a widely promoted strategy to shift consumer demand away from wildlife products derived from threatened species. Yet, there is little evidence on whether product substitution prevents illegal or unsustainable harvesting and contributes to the recovery of threatened populations.
Aditya Shekhar Malgaonkar   +17 more
wiley   +1 more source

Classification of Gene Expression Data Based on Dimension Reduction [PDF]

open access: yes, 2015
利用基因芯片技术能够做到同时对多到数以万计的基因进行并行分析,随着该技术越来越成熟并得到广泛应用,现在已经有越来越多的基因表达数据测定出来,亟需处理,借助于计算机工具以及机器学习方法对这些大量数据进行分析是现在一个很重要的研究领域。其中,基因表达数据的分类研究是该领域的一个热点,通过分类能够帮助研究者发现正常细胞组织与疾病组织之间基因的本质差异,识别致病基因,对基因型疾病的临床诊断和治疗具有重要的意义。 基因表达数据具有“样本少、维数高、分布不平衡”的特点,这给分类带来了很多的困难和挑战 ...
李亮亮
core  

Providing regular and frequent maps of losses and gains of farmland birds based on European monitoring data

open access: yesConservation Biology, EarlyView.
Abstract Knowledge of species distributions is essential for informing policies on nature conservation and restoration. However, updating them on a regular basis and doing so in a harmonized manner at the international level is difficult. The European Bird Census Council integrated national monitoring data covering 5 years to update farmland bird ...
Sergi Herrando   +54 more
wiley   +1 more source

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